LOCUS       sequence-122230-        1184 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Golden Gate Part: Promoter U6 (Triticum aestivum).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Lawrenson T, Shorinola O, Stacey N, Li C, Ostergaard L, Patron N, 
            Uauy C, Harwood W
  TITLE     Induction of targeted, heritable mutations in barley and Brassica 
            oleracea using RNA-guided Cas9 nuclease.
  JOURNAL   Genome Biol. 2015 Nov 30;16(1):258. doi: 10.1186/s13059-015-0826-7.
  PUBMED    26616834
REFERENCE   2  (bases 1 to 1184)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2015-11-30"; volume: "16(1):258. doi"; pages: " 
            10.1186/s13059-015-0826-7"
COMMENT     Sequence Label: pICSL90003
FEATURES             Location/Qualifiers
     source          1..1184
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(618..637)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(619..637)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(648..665)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(648..664)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(657..679)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 tgcttgtctt ggcccccggc tttcccgaac gaccgagcgc ancgagtcag tgagcgagga
       61 agcggagcan cgcccaatac gcaaggaaac agctatgacc atgttaatgc agctggcacg
      121 acaggtttcc cgactggaaa gcgggcagtg aagtgaaggc ccatgaggcc agtcttgtgc
      181 tccaggtacc ggtctcacaa gtctgatgca gcaagcgagt ttataaaccg gtcagaactc
      241 tgctggacag gcgaggtggg actaaactaa gaggaactgc tgcgttgctc tccccaatcg
      301 tgaacacgtc gaccacccga caaaagcccg atcatgcggg cctaaaagcc cagcgctaga
      361 ccgttgctga actcacgtgg gctgcatctc actatgcgac ccagatcagt gagagctaga
      421 gggcagttat cttcagctct ctcgtagttc ctctgatcaa tattcctagt tcgattccct
      481 cctagcagtg agtaacaatg tgctccttga taaatataaa tgtgttgagc accagaactg
      541 tcagaataac gggcttggtc ctcctgagac cgagctctgg acaagacacg tggccttgac
      601 ggccttcact caattcgccc tatagtgagt cgtattacgt cgcgctcact ggccgtcgtt
      661 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
      721 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgaaacgcc cttcccaaca
      781 gttgcgcagc ctgaatggcg aatgggagcg ccctgtagcg gccactcaac cctatctcgg
      841 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
      901 tgatttaaca aaaatttaac gcgaatttaa caaaatntta acgcttacaa tttangtggc
      961 actttttcgg ggaaatgtgc gcggaacccc ctnatttgtt ttattttttc taaatacant
     1021 tcaaatatgt ntcccgctnc tgnaaacaat aaccctgaat aaatgcttca anaanattga
     1081 aaaaaggaaa antnttgngt anccgtaatn gnanccntan cntgaacaaa ncttancggn
     1141 cggttantgt gcnggggccg gtgtttttgg gnngcttnna ttnc
//
