LOCUS       sequence-122233-        8891 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For bacterial expression of recombinant human GTSE1 (longer isoform)
            tagged with MBP and His..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8891)
  AUTHORS   Nixon FM, Gutierrez-Caballero C, Hood FE, Booth DG, Prior IA, Royle 
            SJ
  TITLE     The mesh is a network of microtubule connectors that stabilizes 
            individual kinetochore fibers of the mitotic spindle.
  JOURNAL   Elife. 2015 Jun 19;4. doi: 10.7554/eLife.07635.
  PUBMED    26090906
REFERENCE   2  (bases 1 to 8891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-06-19"; volume: "4. doi"; pages: " 10.7554/eLife.07635"
COMMENT     Sequence Label: MBP-GTSE1(long)-His6
FEATURES             Location/Qualifiers
     source          1..8891
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..80
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             81..1163
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(100..119)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        join(1406..1411,1412..1427,1428..1434)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    1442..1458
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1447..1469
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             1528..2628
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     2602..2625
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             2677..2700
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             4951..4968
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4981..4998)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4981..4997)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4990..5012)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5176..5193)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      5346..5432
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5524..5551
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        5571..5662
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5663..5731,5732..6523)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERSPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5881..5900)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(6565..7078)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6760..6781)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6972..6991
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      7189..7777
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7678..7697
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7931..7948
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7963..8105
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8031..8050)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8191..8213
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8207..8398)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 ccgacaccat cgaatggtgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga
       61 gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg
      121 gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa
      181 cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac
      241 aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc
      301 acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg
      361 tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc
      421 ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca
      481 ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga
      541 cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc
      601 tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg
      661 cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag
      721 cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga
      781 atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa
      841 tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg
      901 acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc
      961 gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga
     1021 agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata
     1081 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     1141 cccgactgga aagcgggcag tgagcgcaac gcaattaatg taagttagct cactcattag
     1201 gcacaattct catgtttgac agcttatcat cgactgcacg gtgcaccaat gcttctggcg
     1261 tcaggcagcc atcggaagct gtggtatggc tgtgcaggtc gtaaatcact gcataattcg
     1321 tgtcgctcaa ggcgcactcc cgttctggat aatgtttttt gcgccgacat cataacggtt
     1381 ctggcaaata ttctgaaatg agctgttgac aattaatcat cggctcgtat aatgtgtgga
     1441 attgtgagcg gataacaatt tcacacagga aacagccagt ccgtttaggt gttttcacga
     1501 gcacttcacc aacaaggacc atagcatatg aaaatcgaag aaggtaaact ggtaatctgg
     1561 attaacggcg ataaaggcta taacggtctc gctgaagtcg gtaagaaatt cgagaaagat
     1621 accggaatta aagtcaccgt tgagcatccg gataaactgg aagagaaatt cccacaggtt
     1681 gcggcaactg gcgatggccc tgacattatc ttctgggcac acgaccgctt tggtggctac
     1741 gctcaatctg gcctgttggc tgaaatcacc ccggacaaag cgttccagga caagctgtat
     1801 ccgtttacct gggatgccgt acgttacaac ggcaagctga ttgcttaccc gatcgctgtt
     1861 gaagcgttat cgctgattta taacaaagat ctgctgccga acccgccaaa aacctgggaa
     1921 gagatcccgg cgctggataa agaactgaaa gcgaaaggta agagcgcgct gatgttcaac
     1981 ctgcaagaac cgtacttcac ctggccgctg attgctgctg acgggggtta tgcgttcaag
     2041 tatgaaaacg gcaagtacga cattaaagac gtgggcgtgg ataacgctgg cgcgaaagcg
     2101 ggtctgacct tcctggttga cctgattaaa aacaaacaca tgaatgcaga caccgattac
     2161 tccatcgcag aagctgcctt taataaaggc gaaacagcga tgaccatcaa cggcccgtgg
     2221 gcatggtcca acatcgacac cagcaaagtg aattatggtg taacggtact gccgaccttc
     2281 aagggtcaac catccaaacc gttcgttggc gtgctgagcg caggtattaa cgccgccagt
     2341 ccgaacaaag agctggcaaa agagttcctc gaaaactatc tgctgactga tgaaggtctg
     2401 gaagcggtta ataaagacaa accgctgggt gccgtagcgc tgaagtctta cgaggaagag
     2461 ttggcgaaag atccacgtat tgccgccact atggaaaacg cccagaaagg tgaaatcatg
     2521 ccgaacatcc cgcagatgtc cgctttctgg tatgccgtgc gtactgcggt gatcaacgcc
     2581 gccagcggtc gtcagactgt cgatgaagcc ctgaaagacg cgcagactaa ttcgagctcg
     2641 aacaacaaca acaataacaa taacaacaac ctcgggctgg aagttctgtt ccaggggccc
     2701 gaattcatgg aaggcggccg cgatgagcct tcagcctgcc gggcagggga cgtgaacatg
     2761 gatgacccta agaaggaaga cattcttctt ttggccgatg aaaaatttga cttcgatctt
     2821 tcattgtctt cttcgagtgc aaatgaagat gatgaagtct tcttcggacc ctttggacat
     2881 aaagaaagat gtattgctgc cagcttggaa ttaaataatc cggttcccga acagcctccg
     2941 ttgcccacat ctgagagtcc ctttgcctgg agccctctgg ccggggagaa gttcgtggag
     3001 gtgtacaaag aagctcactt actggcttta cacattgaga gcagcagccg gaaccaggca
     3061 gcccaagctg ccaagcctga agaccctcgg agccagggcg tggaaagatt catacaggag
     3121 tcaaaattaa aaataaacct ctttgagaaa gaaaaggaaa tgaagaaaag ccccacgtct
     3181 cttaaaaggg agacatacta cctgtcagac agccccttgc tggggccccc tgtgggtgag
     3241 cctcggctct tggcctcctc cccggccctg cccagctctg gtgcccaggc ccgcctcacc
     3301 cgggcgccgg ggcctccgca ctctgctcat gctttgccca gggaatcatg cactgctcat
     3361 gctgcaagtc aggcagcgac tcagaggaag cccgggacca aattgctgct gcctcgagcg
     3421 gcctctgtta gaggaagaag catccctggg gctgcggaga agcccaagaa agagattcca
     3481 gctagtcctt ccaggacaaa aatcccagct gagaaggaat cccaccggga tgttctccct
     3541 gacaaacctg ccccgggtgc tgtcaatgtg ccggccgccg gaagccactt gggccagggc
     3601 aagcgggcga tccctgttcc aaacaagttg gggctgaaga agaccctgtt aaaagcaccc
     3661 ggctctacca gcaatctcgc aaggaagtcc tcctcggggc ctgtttggag cggggcatcc
     3721 agtgcgtgca catccccagc agtgggcaaa gctaaatcaa gtgaatttgc aagtattcct
     3781 gcaaatagct cccggcctct gtcaaacatc agcaagtcag gcagaatggg acccgccatg
     3841 ctgcggccag ctctgcctgc aggccctgtg ggggcatcct cctggcaggc caagcgggtc
     3901 gatgtttctg agctggcagc ggagcagctc acggcacccc cctcagcatc ccccacccaa
     3961 ccccagactc cggaaggtgg cggccagtgg ctgaactcca gttgcgcttg gtcagaatct
     4021 tctcaattga ataagactag aagtatcaga cggcgagatt cctgtctaaa ttccaagaca
     4081 aaggttatgc ctactcctac aaatcaattt aaaattccta agttttctat tggtgactcc
     4141 ccggacagct caacaccaaa gctttcgcgg gcacagcggc cgcagtcgtg cacgtcagtt
     4201 ggcagggtca ctgtccacag caccccggtt agacgctcat ctgggccagc accacaaagc
     4261 ctgctgagcg cacggcgtgt gtcagccttg cccacacccg ccagccggcg ctgctctggc
     4321 cttccaccga tgacccccaa aacgatgccc agggccgtgg gctctcccct gtgtgtgcca
     4381 gctcggagac gttcctctga gccccgcaag aactctgcaa tgagaactga accaacaagg
     4441 gagagcaaca gaaagacaga ttccaggctg gtggatgtgt cccctgacag gggttctcct
     4501 ccttcccgtg tgcctcaggc acttaacttt tctccagagg aaagcgattc tactttctcc
     4561 aaaagtactg ccacagaagt agctcgggag gaagccaagc cgggtggaga tgcagcccct
     4621 agtgaggctc ttcttgtaga tatcaaactg gaaccactcg cggtcactcc agatgctgca
     4681 agccagcccc tcattgacct tcctctcatc gacttctgcg ataccccaga agcacacgtg
     4741 gctgtaggat ctgaaagcag gcctctgatc gacctcatga caaacactcc agacatgaat
     4801 aaaaatgtgg ccaaaccttc accggtggtg ggacagctca tagacctgag ctcccctctg
     4861 atccagctga gccctgaggc tgacaaggag aacgtggatt ccccactcct caagttctgg
     4921 atcctggccg gcctggcggc cgcagtcgac caccatcacc atcaccatta aaagcttggc
     4981 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     5041 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     5101 cccttcccaa cagttgcgca gcctgaatgg cgaatggcag cttggctgtt ttggcggatg
     5161 agataagatt ttcagcctga tacagattaa atcagaacgc agaagcggtc tgataaaaca
     5221 gaatttgcct ggcggcagta gcgcggtggt cccacctgac cccatgccga actcagaagt
     5281 gaaacgccgt agcgccgatg gtagtgtggg gtctccccat gcgagagtag ggaactgcca
     5341 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
     5401 tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg aacgttgcga
     5461 agcaacggcc cggagggtgg cgggcaggac gcccgccata aactgccagg catcaaatta
     5521 agcagaaggc catcctgacg gatggccttt ttgcgtttct acaaactctt tttgtttatt
     5581 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     5641 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     5701 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     5761 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     5821 gatccttgag agttttcgcc ccgaagaacg ttctccaatg atgagcactt ttaaagttct
     5881 gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat
     5941 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     6001 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     6061 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     6121 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     6181 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     6241 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     6301 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     6361 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     6421 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     6481 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     6541 ctcatatata ctttagattg atttaccccg gttgataatc agaaaagccc caaaaacagg
     6601 aagattgtat aagcaaatat ttaaattgta aacgttaata ttttgttaaa attcgcgtta
     6661 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     6721 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     6781 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     6841 ccactacgtg aaccatcacc caaatcaagt tttttggggt cgaggtgccg taaagcacta
     6901 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     6961 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
     7021 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtaa
     7081 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     7141 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     7201 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     7261 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     7321 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     7381 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     7441 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     7501 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     7561 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     7621 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     7681 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     7741 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     7801 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     7861 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     7921 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct
     7981 ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc
     8041 tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg
     8101 ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     8161 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     8221 cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgcagcg
     8281 attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta
     8341 atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg
     8401 atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga
     8461 ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg
     8521 gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc
     8581 agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc
     8641 agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac
     8701 ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc
     8761 ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc
     8821 ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc
     8881 tgcccgaaat t
//
