LOCUS       sequence-122236-         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  storage vector for BASIC part: J23102.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Storch M, Casini A, Mackrow B, Fleming T, Trewhitt H, Ellis T, 
            Baldwin GS
  TITLE     BASIC: A New Biopart Assembly Standard for Idempotent Cloning 
            Provides Accurate, Single-Tier DNA Assembly for Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2015 Apr 16.
  PUBMED    25746445
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 16."
COMMENT     Sequence Label: BASIC_6_J23102
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    111..132
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        133..167
                     /label=BBa_J23102
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.86"
     promoter        complement(join(628..634,635..652,653..658))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    673..694
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(814..831)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agattctggt gggtctctgt ccagaccttt acgccgctgg agactagtca cagctaacac
       61 cacgtcgtcc ctatctgctg ccctaggtct atgagtggtt gctggataac gaattcgcgg
      121 ccgcttctag agttgacagc tagctcagtc ctaggtactg tgctagctac tagagggctc
      181 gggagaccta tcgatctttc tagaagatct cctacaatat tctcagctgc catggaaaat
      241 cgatgttctt cttttattct ctcaagattt tcaggctgta tattaaaact tatattaaga
      301 actatgctaa ccacctcatc aggaaccgtt gtaggtggcg tgggttttct tggcaatcga
      361 ctctcatgaa aactacgagc taaatattca atatgttcct cttgaccaac tttattctgc
      421 attttttttg aacgaggttt agagcaagct tcaggaaact gagacaggaa ttttattaaa
      481 aatttaaatt ttgaagaaag ttcagggtta atagcatcca ttttttgctt tgcaagttcc
      541 tcagcattct taacaaaaga cgtctctttt gacatgttta aagtttaaac ctcctgtgtg
      601 aaattattat ccgctcataa ttccacacat tatacgagcc ggaagcataa agtgtaaagc
      661 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgccaattgc
      721 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
      781 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
      841 cgttcggctg cggcgagcgg tatcagctca ctcaa
//
