LOCUS       sequence-122238-         783 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The prostate specific promoter controls expression of FlpO linked to
            a blue flourescent protein. gRNA towards PTEN ex5, p53 ex7 and 8, 
            and SMAD4 ex2 and ex 9 are expressed by the U6 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 783)
  TITLE     Cas9/CRISPR pig transposon 
            plasmid_LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 5xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2,...
FEATURES             Location/Qualifiers
     source          1..783
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        263..511
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     434..453
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        532..607
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 gtaccctaca gttgaagtcg gaagtttaca tacacttaag ttggagtcat taaaactcgt
       61 ttttcaacta ctccacaaat ttcttgttaa caaacaatag ttttggcaag tcagttagga
      121 catctacttt gtgcatgaca caagtcattt ttccaacaat tgtttacaga cagattattt
      181 cacttataat tcactgtatc acaattccag tgggtcagaa gtttacatac actaagttga
      241 ctgtgccttt aaacagtcgc gagagggcct atttcccatg attccttcat atttgcatat
      301 acgatacaag gctgttagag agataattgg aattaatttg actgtaaaca caaagatatt
      361 agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag ttttaaaatt
      421 atgttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg atttcttggc
      481 tttatatatc ttgtggaaag gacgaaacac cgcagcaatt cactgtaaag cgttttagag
      541 ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag
      601 tcggtgcttt tttaccggtg agggcctatt tcccatgatt ccttcatatt tgcatatacg
      661 atacaaggct gttagagaga taattggaat taatttgact gtaaacacaa agatattagt
      721 acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg
      781 ttt
//
