LOCUS       sequence-122245-         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The prostate specific promoter controls expression of FlpO linked to
            a blue flourescent protein. gRNA towards PTEN ex5, p53 ex8 and SMAD4
            ex2. are expressed by the U6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  TITLE     Cas9/CRISPR pig transposon 
            plasmid_LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 3xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2
            ...
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        264..512
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     435..454
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        533..608
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 agtaccctac agttgaagtc ggaagtttac atacacttaa gttggagtca ttaaaactcg
       61 tttttcaact actccacaaa tttcttgtta acaaacaata gttttggcaa gtcagttagg
      121 acatctactt tgtgcatgac acaagtcatt tttccaacaa ttgtttacag acagattatt
      181 tcacttataa ttcactgtat cacaattcca gtgggtcaga agtttacata cactaagttg
      241 actgtgcctt taaacagtcg cgagagggcc tatttcccat gattccttca tatttgcata
      301 tacgatacaa ggctgttaga gagataattg gaattaattt gactgtaaac acaaagatat
      361 tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat
      421 tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg
      481 ctttatatat cttgtggaaa ggacgaaaca ccgcagcaat tcactgtaaa gcgttttaga
      541 gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga
      601 gtcggtgctt ttttaccggt gagggcctat ttcccatgat tccttcatat ttgcatatac
      661 gatacaaggc tgttagagag ataattggaa ttaatttgac tgtaaacaca aagatattag
      721 tacaaaatac gtgacgtaga aagtaataat ttcttgggta gtttgcagtt ttaaaattat
      781 gttttaaa
//
