LOCUS       sequence-122249-        1041 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PuroR containing plasmid for the generation of gene disruptions via 
            Bxb1-mediated recombination into MIN-tagged cell lines. This vector 
            can also be used to express cDNAs..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Mulholland CB, Smets M, Schmidtmann E, Leidescher S, Markaki Y, 
            Hofweber M, Qin W, Manzo M, Kremmer E, Thanisch K, Bauer C, Rombaut 
            P, Herzog F, Leonhardt H, Bultmann S
  TITLE     A modular open platform for systematic functional studies under 
            physiological conditions.
  JOURNAL   Nucleic Acids Res. 2015 May 24. pii: gkv550.
  PUBMED    26007658
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 24. pii: gkv550."
COMMENT     Sequence Label: attB‐mCh­‐Poly(A)-­PuroR
FEATURES             Location/Qualifiers
     source          1..1041
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      63..72
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(118..822)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(393..412)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     662..680
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     protein_bind    825..862
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
ORIGIN
        1 tgtctggatc ctctagagaa ttcgttaact gcggccgcac tagtcaattc ccgggcgatc
       61 gcccatggtg gcgtccacgg cgctgttgct cgcctgggct ctgctcctca ggccgctctt
      121 gtacagctcg tccatgccgc cggtggagtg gcggccctcg gcgcgttcgt actgttccac
      181 gatggtgtag tcctcgttgt gggaggtgat gtccaacttg atgttgacgt tgtaggcgcc
      241 gggcagctgc acgggcttct tggccttgta ggtggtcttg acctcagcgt cgtagtggcc
      301 gccgtccttc agcttcagcc tctgcttgat ctcgcccttc agggcgccgt cctcggggta
      361 catccgctcg gaggaggcct cccagcccat ggtcttcttc tgcattacgg ggccgtcgga
      421 ggggaagttg gtgccgcgca gcttcacctt gtagatgaac tcgccgtcct gcagggagga
      481 gtcctgggtc acggtcacca cgccgccgtc ctcgaagttc atcacgcgct cccacttgaa
      541 gccctcgggg aaggacagct tcaagtagtc ggggatgtcg gcggggtgct tcacgtaggc
      601 cttggagccg tacatgaact gaggggacag gatgtcccag gcgaagggca gggggccacc
      661 cttggtcacc ttcagcttgg cggtctgggt gccctcgtag gggcggccct cgccctcgcc
      721 ctcgatctcg aactcgtggc cgttcacgga gccctccatg tgcaccttga agcgcatgaa
      781 ctccttgatg atggccatgt tatcctcctc gcccttgctc acggatgatc ctgacgacgg
      841 agaccgccgt cgtcgacaag ccgggatgct ggttctttcc gcctcagaag ccatagagcc
      901 caccgcatcc ccagcatgcc tgctattgtc ttcccaatcc tcccccttgc tgtcctgccc
      961 caccccaccc cccagaatag aatgacacct actcagacaa tgcgatgcaa tttcctcatt
     1021 ttattaggaa aggacagtgg g
//
