LOCUS       sequence-122251-         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Flag tagged mammalian expression of GC domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Tyagarajan SK, Ghosh H, Yevenes GE, Imanishi SY, Zeilhofer HU, 
            Gerrits B, Fritschy JM
  TITLE     Extracellular signal-regulated kinase and glycogen synthase kinase 
            3beta regulate gephyrin postsynaptic aggregation and GABAergic 
            synaptic function in a calpain-dependent mechanism.
  JOURNAL   J Biol Chem. 2013 Apr 5;288(14):9634-47. doi: 
            10.1074/jbc.M112.442616. Epub 2013 Feb 13.
  PUBMED    23408424
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.442616"; journalName: "J Biol Chem"; date: 
            "2013-04-5- 5"; volume: "288"; issue: "14"; pages: "9634-47"
COMMENT     Sequence Label: pCR3-FLAG-Gephyrin GC
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             71..142
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttatg gactacaagg acgacgacga caaggactac aaggacgacg acgacaagga
      121 ctacaaggac gacgacgaca agggagcagg agcgaccgag ggaatgatcc tcacaaacca
      181 cgaccatcaa atccgtgtcg gagtcctcac agtgagtgat agctgcttca ggaatcttgc
      241 agaagaccgc agtgggataa atctaaaaga tctcgtccag gatccttctt tgttgggtgg
      301 gactatatca gcatacaaga tagtaccaga tgaaatagaa gaaatcaagg aaacactcat
      361 agactggtgt gatgagaagg aacttaattt gatattaaca actggcggaa cagggtttgc
      421 accacgagat gtcactccag aggccacaaa agaagtaata gaacgggaag cgccagggat
      481 ggccctggca atgctgatgg gatcacttaa tgttacacct ctgggcatgc tctctagacc
      541 agtgtgtgga ataagaggga aaactctgat aattaacctg cctggtagca agaaaggatc
      601 tcaggaatgc tttcagttca tactgccagc tctacctcat gccattgacc ttttacgtga
      661 tgccattgta aaagtaaagg aggtgcatga tgaacttgaa gatttacctt ccccaccacc
      721 tcctctttct ccacctccta caactagccc acataagcag acagaagaca a
//
