LOCUS       sequence-122253-       12767 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For bacterial expression of recombinant human ch-TOG tagged with MBP
            and His..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12767)
  AUTHORS   Nixon FM, Gutierrez-Caballero C, Hood FE, Booth DG, Prior IA, Royle 
            SJ
  TITLE     The mesh is a network of microtubule connectors that stabilizes 
            individual kinetochore fibers of the mitotic spindle.
  JOURNAL   Elife. 2015 Jun 19;4. doi: 10.7554/eLife.07635.
  PUBMED    26090906
REFERENCE   2  (bases 1 to 12767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-06-19"; volume: "4. doi"; pages: " 10.7554/eLife.07635"
COMMENT     Sequence Label: MBP-ch-TOG-His6
FEATURES             Location/Qualifiers
     source          1..12767
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..80
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             81..1163
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(100..119)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        join(1406..1411,1412..1427,1428..1434)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    1442..1458
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1447..1469
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             1528..2628
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     2602..2625
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             2677..2700
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(3479..3496)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             8827..8844
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(8857..8874)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8857..8873)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8866..8888)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(9052..9069)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      9222..9308
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      9400..9427
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        9447..9538
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9539..9607,9608..10399)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERSPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9757..9776)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(10441..10954)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10636..10657)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10848..10867
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      11065..11653
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11554..11573
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11807..11824
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    11839..11981
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(11907..11926)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     12067..12089
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(12083..12274)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 ccgacaccat cgaatggtgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga
       61 gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg
      121 gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa
      181 cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac
      241 aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc
      301 acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg
      361 tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc
      421 ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca
      481 ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga
      541 cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc
      601 tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg
      661 cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag
      721 cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga
      781 atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa
      841 tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg
      901 acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc
      961 gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga
     1021 agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata
     1081 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     1141 cccgactgga aagcgggcag tgagcgcaac gcaattaatg taagttagct cactcattag
     1201 gcacaattct catgtttgac agcttatcat cgactgcacg gtgcaccaat gcttctggcg
     1261 tcaggcagcc atcggaagct gtggtatggc tgtgcaggtc gtaaatcact gcataattcg
     1321 tgtcgctcaa ggcgcactcc cgttctggat aatgtttttt gcgccgacat cataacggtt
     1381 ctggcaaata ttctgaaatg agctgttgac aattaatcat cggctcgtat aatgtgtgga
     1441 attgtgagcg gataacaatt tcacacagga aacagccagt ccgtttaggt gttttcacga
     1501 gcacttcacc aacaaggacc atagcatatg aaaatcgaag aaggtaaact ggtaatctgg
     1561 attaacggcg ataaaggcta taacggtctc gctgaagtcg gtaagaaatt cgagaaagat
     1621 accggaatta aagtcaccgt tgagcatccg gataaactgg aagagaaatt cccacaggtt
     1681 gcggcaactg gcgatggccc tgacattatc ttctgggcac acgaccgctt tggtggctac
     1741 gctcaatctg gcctgttggc tgaaatcacc ccggacaaag cgttccagga caagctgtat
     1801 ccgtttacct gggatgccgt acgttacaac ggcaagctga ttgcttaccc gatcgctgtt
     1861 gaagcgttat cgctgattta taacaaagat ctgctgccga acccgccaaa aacctgggaa
     1921 gagatcccgg cgctggataa agaactgaaa gcgaaaggta agagcgcgct gatgttcaac
     1981 ctgcaagaac cgtacttcac ctggccgctg attgctgctg acgggggtta tgcgttcaag
     2041 tatgaaaacg gcaagtacga cattaaagac gtgggcgtgg ataacgctgg cgcgaaagcg
     2101 ggtctgacct tcctggttga cctgattaaa aacaaacaca tgaatgcaga caccgattac
     2161 tccatcgcag aagctgcctt taataaaggc gaaacagcga tgaccatcaa cggcccgtgg
     2221 gcatggtcca acatcgacac cagcaaagtg aattatggtg taacggtact gccgaccttc
     2281 aagggtcaac catccaaacc gttcgttggc gtgctgagcg caggtattaa cgccgccagt
     2341 ccgaacaaag agctggcaaa agagttcctc gaaaactatc tgctgactga tgaaggtctg
     2401 gaagcggtta ataaagacaa accgctgggt gccgtagcgc tgaagtctta cgaggaagag
     2461 ttggcgaaag atccacgtat tgccgccact atggaaaacg cccagaaagg tgaaatcatg
     2521 ccgaacatcc cgcagatgtc cgctttctgg tatgccgtgc gtactgcggt gatcaacgcc
     2581 gccagcggtc gtcagactgt cgatgaagcc ctgaaagacg cgcagactaa ttcgagctcg
     2641 aacaacaaca acaataacaa taacaacaac ctcgggctgg aagttctgtt ccaggggccc
     2701 gaattcggga tcctaatggg agatgacagt gagtggttga aactgccagt tgatcagaaa
     2761 tgtgaacaca agctgtggaa agcaaggtta agtgggtatg aagaggccct gaagatcttc
     2821 cagaaaataa aggatgaaaa gagcccagag tggtccaaat ttttaggatt gatcaaaaaa
     2881 tttgtcactg attccaatgc agtggttcaa ttgaaaggat tagaagctgc acttgtttat
     2941 gttgaaaatg cccatgtagc aggaaaaacc acaggagaag ttgtgtcagg tgttgtaagt
     3001 aaggtgttca atcaacctaa agctaaagcc aaggagctgg gcatagagat ctgtcttatg
     3061 tacatagaga ttgagaaagg agaggctgtt caagaagagc tcctgaaagg cttggacaat
     3121 aagaatccca agatcatagt ggcctgtata gagacactga ggaaagcctt aagtgaattt
     3181 ggttccaaaa tcatcttgct taagccaatt atcaaagtgt tgccaaaact ctttgagtct
     3241 cgagagaagg ctgttcgaga tgaagccaaa ctaattgctg tggagattta cagatggatt
     3301 cgggatgctc tgagaccccc attacaaaat ataaactctg ttcagttgaa agaactagaa
     3361 gaagaatggg tcaaactgcc aacaagtgct cctagaccta ctcgatttct tcgttcccaa
     3421 caagaactag aagctaaatt ggaacaacaa cagtctgctg gtggagatgc tgaaggaggt
     3481 ggtgatgatg gtgatgaggt gccacaaata gatgcttatg agcttttaga agctgtagaa
     3541 atcctttcca aacttcccaa agacttttat gacaaaattg aggcaaaaaa atggcaagag
     3601 agaaaagagg ccctggagtc tgtagaagta ctaataaaaa accccaaact ggaagctggc
     3661 gattatgcag atttagtaaa agcattaaag aaggttgttg gaaaggacac caatgtcatg
     3721 ttggtggctt tggcagcaaa atgtcttact ggcctggctg ttgggctaag gaagaaattt
     3781 ggacaatatg caggacatgt tgtgccaacc atcttggaga aattcaaaga gaagaaacct
     3841 caagtggtac aagccctgca ggaggcaatt gatgcaatct tccttactac cacactacag
     3901 aacatcagtg aggatgtttt agcagtaatg gataataaaa atccaaccat caagcagcag
     3961 acatctcttt ttattgcaag aagtttccgc cactgcactg cttctaccct gccaaagagc
     4021 ttgctaaagc ccttttgtgc tgcactactt aagcacatca atgattctgc tcctgaagtc
     4081 agagatgccg catttgaagc attgggtact gctttgaagg tggttggcga gaaagcagta
     4141 aacccattcc tagctgatgt ggacaaactc aagcttgata agatcaaaga atgttcagaa
     4201 aaggtagaac tgatacatgg taagaaagct ggactagctg ctgataagaa ggaattcaaa
     4261 cctctgcctg gaaggactgc tgcttcaggg gctgcaggag ataaggacac aaaggacatt
     4321 tctgcaccca aaccaggacc tctaaaaaag gcacctgctg ctaaggctgg tgggccacca
     4381 aaaaagggga aaccagctgc accaggaggc gcagggaata ctggaaccaa gaacaagaaa
     4441 ggactggaga ctaaagaaat agtggagcct gagctctcga tagaagtatg tgaagaaaaa
     4501 gcttcagctg ttcttccccc tacctgtata cagcttcttg acagcagtaa ctggaaagaa
     4561 aggctggctt gtatggaaga gttccagaag gctgttgagc taatggaccg aactgaaatg
     4621 ccatgccagg cattagtgag gatgctagcc aagaaacctg gatggaaaga aactaatttt
     4681 caggtgatgc aaatgaagct tcatatagtt gctttgattg cccagaaggg aaatttttcc
     4741 aaaacgtcag ctcaggttgt attagatggc cttgtggaca agattggaga tgtgaaatgt
     4801 gggaacaatg caaaagaagc tatgacagca atagccgaag cctgtatgtt accatggact
     4861 gctgaacagg ttgtgtcaat ggctttctca caaaagaatc ccaaaaatca gtcagaaact
     4921 ctgaattggc tatcaaatgc cataaaagaa tttggttttt ctgggttgaa tgtcaaagct
     4981 ttcattagca atgtgaagac agctcttgct gcaacaaacc cagctgtgag gactgctgcc
     5041 ataaccctgc ttggcgtgat gtatctgtat gttggtccct ctttgcgaat gttctttgag
     5101 gatgagaagc ctgccctcct atcccagata gatgcagaat ttgagaagat gcagggacaa
     5161 agtccacctg ctccaaccag aggaatttcc aagcatagca caagtggtac agatgaagga
     5221 gaagatggag atgaaccaga tgacgggagc aatgatgtcg ttgatctttt gccgaggacg
     5281 gagatcagtg ataaaatcac ttcagagttg gtatctaaga ttggtgataa gaattggaag
     5341 attaggaaag aaggcctaga tgaagtggca ggtattatta atgacgcaaa atttatccaa
     5401 ccgaatatag gtgaacttcc aactgccttg aagggtcgac tcaatgattc aaataaaatc
     5461 ttggtacagc aaacgctgaa tatcctgcaa caactggcag tagccatggg cccaaatatt
     5521 aagcaacatg taaaaaattt aggcatccct atcatcacag tccttggaga cagcaagaac
     5581 aatgttcgag ctgctgccct agcgactgtg aatgcttggg cagaacagac tggcatgaag
     5641 gaatggctgg aaggagaaga tctttctgaa gagctcaaaa aggaaaatcc tttcttgagg
     5701 caagagcttc tgggctggct ggctgagaaa ctacctactc ttcgttccac ccctacagac
     5761 cttatccttt gtgttcctca tctctactcc tgcctagaag atcgaaatgg agatgtgcga
     5821 aagaaggccc aagatgcctt gccattcttc atgatgcatt taggatatga aaaaatggcc
     5881 aaggctactg ggaaactaaa gccaacttct aaagatcagg tattggccat gctagagaaa
     5941 gccaaagtta acatgccagc caagcctgct ccacccacta aagcaacttc taaaccaatg
     6001 ggagggtccg ctccagccaa attccagcct gcatcagcac ctgctgaaga ttgtatttcc
     6061 agcagtacag aacccaaacc tgatccaaaa aaggccaaag ctccaggatt atcctctaaa
     6121 gcaaagagtg cacaagggaa gaagatgcca agcaaaacca gcttaaagga ggatgaagac
     6181 aaatccgggc ctatttttat tgttgttcca aatggaaaag agcaaaggat gaaagatgaa
     6241 aaaggattga aggtgctaaa gtggaatttt actaccccac gggatgaata cattgagcaa
     6301 ctaaagactc aaatgtctag ctgtgtggct aaatggttac aagatgagat gtttcactca
     6361 gactttcagc atcataacaa agcccttgct gttatggttg atcacttgga gagtgaaaaa
     6421 gaaggagtta ttggttgcct ggatcttatc ttaaagtggc ttaccctgag gttttttgac
     6481 accaatacaa gcgtcctgat gaaagcacta gaatatttaa aattgctctt caccttgcta
     6541 agtgaagaag aatatcatct tactgagaat gaagcatctt ccttcatccc ctatcttgtc
     6601 gtcaaggttg gagaaccaaa ggatgtcatt cgtaaagatg ttcgtgccat cctgaaccgg
     6661 atgtgccttg tctacccagc tagcaagatg tttcccttta tcatggaagg aaccaaatcc
     6721 aaaaactcta agcagagagc agagtgcctg gaagagctgg gatgtctggt tgagtcctat
     6781 ggcatgaatg tttgccaacc aaccccagga aaagccttaa aggaaatagc tgttcacata
     6841 ggagaccgtg acaatgctgt acgcaatgct gcactcaaca ccattgtaac ggtgtacaat
     6901 gtacatgggg atcaggtgtt caaactgatt ggaaatcttt ctgaaaagga tatgagcatg
     6961 ctcgaggaga ggattaagcg gtcagcaaag agaccctctg ctgcaccaat aaaacaggtg
     7021 gaagagaaac ctcagcgtgc acagaacata agctccaatg ccaacatgtt acgcaaggga
     7081 ccagctgagg acatgtcttc caaactcaac caagcccgaa gcatgagtgg gcatcctgag
     7141 gcagcccaga tggtccgccg agaattccag ctggatctag atgagattga gaatgacaat
     7201 ggtacagtcc gatgtgaaat gccagaactt gttcagcaca aactggatga catttttgag
     7261 ccagtcctta ttcctgaacc caagatccgg gctgtttctc cacacttcga tgacatgcac
     7321 agtaatacag catccacaat caatttcatt atctcccaag tagccagtgg tgacatcaac
     7381 acaagtatcc aagctctgac acagatcgat gaggtcctga gacaggaaga caaagctgaa
     7441 gccatgtccg gccatattga tcagtttctg atagccactt ttatgcagct aagactcatc
     7501 tacaacacac acatggcaga tgagaaattg gagaaggacg agatcatcaa gttgtatagc
     7561 tgtatcattg gcaacatgat ttcgctgttt cagatagaga gccttgcccg ggaggcctcc
     7621 actggagtac taaaagacct aatgcatggc ctcatcacct taatgctgga ttctcggatt
     7681 gaagatcttg aggaaggaca acaggtcatc cgctctgtga acctcttggt ggtgaaggtt
     7741 ctggagaagt cagaccagac caacatcctg agtgccctac ttgttttgct ccaagacagc
     7801 ctgctagcaa cagccagttc tcccaaattc tcagagcttg ttatgaagtg tctctggaga
     7861 atggttcgac tgttgcctga taccatcaat agcattaacc tagacagaat tcttctggat
     7921 atccacattt tcatgaaggt cttccccaaa gagaaactga agcaatgcaa aagtgaattt
     7981 cccataagga ccctaaagac cctgctacac accttatgca aattaaaagg gcccaagatc
     8041 ctggaccacc taacgatgat cgacaacaaa aacgagtctg agctggaggc ccatctctgc
     8101 cggatgatga agcacagtat ggaccagact gggagcaagt ctgataagga aacagaaaag
     8161 ggagcatctc gaatagatga aaaatcatca aaggccaaag tgaatgattt cttagctgag
     8221 atttttaaga agattggctc taaagaaaac actaaagagg gactagcaga gttatatgaa
     8281 tataagaaga aatactcaga tgctgacatt gaaccatttc tgaaaaattc ctcacagttc
     8341 ttccagagct atgtcgaaag aggccttcgg gtgattgaga tggagaggga gggcaaaggt
     8401 cgtatttcca cttcaacagg catctcccct cagatggaag tcacatgtgt gcccacgccc
     8461 acaagcacag tgtcctccat aggtaacaca aatggggaag aagtggggcc atctgtctac
     8521 ttggaaaggc taaagatcct ccgacagcga tgtggtctgg acaacacaaa gcaagatgac
     8581 cgacctcctt tgacctcttt gctctccaaa ccagcagttc ctactgtcgc ctcttccaca
     8641 gacatgctcc acagcaaact ctctcagctc cgggagtcac gggagcagca ccagcattca
     8701 gacctggatt ctaaccagac tcactcttca ggaactgtga cctcctcctc ctccacagct
     8761 aacatagacg acttgaaaaa aagactggag agaataaaga gcagtcgcaa atcggccgca
     8821 gtcgaccacc atcaccatca ccattaaaag cttggcactg gccgtcgttt tacaacgtcg
     8881 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     8941 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     9001 gaatggcgaa tggcagcttg gctgttttgg cggatgagat aagattttca gcctgataca
     9061 gattaaatca gaacgcagaa gcggtctgat aaaacagaat ttgcctggcg gcagtagcgc
     9121 ggtggtccca cctgacccca tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag
     9181 tgtggggtct ccccatgcga gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc
     9241 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta
     9301 ggacaaatcc gccgggagcg gatttgaacg ttgcgaagca acggcccgga gggtggcggg
     9361 caggacgccc gccataaact gccaggcatc aaattaagca gaaggccatc ctgacggatg
     9421 gcctttttgc gtttctacaa actctttttg tttatttttc taaatacatt caaatatgta
     9481 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     9541 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     9601 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     9661 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     9721 agaacgttct ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     9781 tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     9841 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     9901 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     9961 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
    10021 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
    10081 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
    10141 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
    10201 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
    10261 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
    10321 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
    10381 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
    10441 accccggttg ataatcagaa aagccccaaa aacaggaaga ttgtataagc aaatatttaa
    10501 attgtaaacg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
    10561 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
    10621 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
    10681 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcacccaaa
    10741 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
    10801 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
    10861 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
    10921 cccgccgcgc ttaatgcgcc gctacagggc gcgtaaaagg atctaggtga agatcctttt
    10981 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
    11041 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
    11101 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
    11161 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
    11221 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
    11281 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
    11341 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
    11401 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
    11461 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
    11521 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
    11581 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
    11641 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
    11701 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
    11761 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
    11821 cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
    11881 acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
    11941 tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac
    12001 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
    12061 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc
    12121 agctgcggta aagctcatca gcgtggtcgt gcagcgattc acagatgtct gcctgttcat
    12181 ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg
    12241 ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc
    12301 tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg
    12361 atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc
    12421 ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag
    12481 gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg
    12541 gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg
    12601 ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg
    12661 attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga
    12721 gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgaaatt
//
