LOCUS       sequence-122254-         979 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PuroR containing plasmid for the generation of gene disruptions via 
            Bxb1-mediated recombination into MIN-tagged cell lines. This vector 
            can also be used to express cDNAs..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Mulholland CB, Smets M, Schmidtmann E, Leidescher S, Markaki Y, 
            Hofweber M, Qin W, Manzo M, Kremmer E, Thanisch K, Bauer C, Rombaut 
            P, Herzog F, Leonhardt H, Bultmann S
  TITLE     A modular open platform for systematic functional studies under 
            physiological conditions.
  JOURNAL   Nucleic Acids Res. 2015 May 24. pii: gkv550.
  PUBMED    26007658
REFERENCE   2  (bases 1 to 979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 24. pii: gkv550."
COMMENT     Sequence Label: attB‐mCh­‐Poly(A)-­PuroR
FEATURES             Location/Qualifiers
     source          1..979
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgccggatcc atgcccacgc
       61 tactgcgggt ttatatagac ggtccccacg ggatggggaa aaccaccacc acgcaactgc
      121 tggtggccct gggttcgcgc gacgatatcg tctacgtacc cgagccgatg acttactggc
      181 gggtgctggg ggcttccgag acaatcgcga acatctacac cacacaacac cgcctcgacc
      241 agggtgagat atcggccggg gacgcggcgg tggtaatgac aagcgcccag ataacaatgg
      301 gcatgcctta tgccgtgacc gacgccgttc tggctcctca tatcgggggg gaggctggga
      361 gctcacatgc cccgcccccg gccctcaccc tcatcttcga ccgccatccc atcgccgccc
      421 tcctgtgcta cccggccgcg cggtacctta tgggcagcat gaccccccag gccgtgctgg
      481 cgttcgtggc cctcatcccg ccgaccttgc ccggcaccaa catcgtgctt ggggcccttc
      541 cggaggacag acacatcgac cgcctggcca aacgccagcg ccccggcgag cggctggacc
      601 tggctatgct ggctgcgatt cgccgcgttt acgggctact tgccaatacg gtgcggtatc
      661 tgcagtgcgg cgggtcgtgg cgggaggact ggggacagct ttcggggacg gccgtgccgc
      721 cccagggtgc cgagccccag agcaacgcgg gcccacgacc ccatatcggg gacacgttat
      781 ttaccctgtt tcgggccccc gagttgctgg cccccaacgg cgacctgtat aacgtgtttg
      841 cctgggcctt ggacgtcttg gccaaacgcc tccgttccat gcacgtcttt atcctggatt
      901 acgaccaatc gcccgccggc tgccgggacg ccctgctgca acttacctcc gggatggtcc
      961 agacccacgt caccacccc
//
