LOCUS       sequence-122259-        1020 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for Bxb1-mediated recombination of the GFP-Dnmt3b1 cDNA into
            a MIN-tagged locus using Neomycin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Mulholland CB, Smets M, Schmidtmann E, Leidescher S, Markaki Y, 
            Hofweber M, Qin W, Manzo M, Kremmer E, Thanisch K, Bauer C, Rombaut 
            P, Herzog F, Leonhardt H, Bultmann S
  TITLE     A modular open platform for systematic functional studies under 
            physiological conditions.
  JOURNAL   Nucleic Acids Res. 2015 May 24. pii: gkv550.
  PUBMED    26007658
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 24. pii: gkv550."
COMMENT     Sequence Label: attB-GFP-Dnmt3b1-Poly(A)-NeoR
FEATURES             Location/Qualifiers
     source          1..1020
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      72..81
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             161..172
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 ctctcggcat ggacgagctg tacaagagcg gcctgaggag cagagcccag gcgagcaaca
       61 gcgccgtgga cgccaccatg ggcgatcgca tgaagggaga cagcagacat ctgaatgaag
      121 aagagggtgc cagcgggtat gaggagtgca ttatcgttaa tgggaacttc agtgaccagt
      181 cctcagacac gaaggatgct ccctcacccc cagtcttgga ggcaatctgc acagagccag
      241 tctgcacacc agagaccaga ggccgcaggt caagctcccg gctgtctaag agggaggtct
      301 ccagccttct gaattacacg caggacatga caggagatgg agacagagat gatgaagtag
      361 atgatgggaa tggctctgat attctaatgc caaagctcac ccgtgagacc aaggacacca
      421 ggacgcgctc tgaaagcccg gctgtccgaa cccgacatag caatgggacc tccagcttgg
      481 agaggcaaag agcctccccc agaatcaccc gaggtcggca gggccgccac catgtgcagg
      541 agtaccctgt ggagtttccg gctaccaggt ctcggagacg tcgagcatcg tcttcagcaa
      601 gcacgccatg gtcatcccct gccagcgtcg acttcatgga agaagtgaca cctaagagcg
      661 tcagtacccc atcagttgac ttgagccagg atggagatca ggagggtatg gataccacac
      721 aggtggatgc agagagcaga gatggagaca gcacagagta tcaggatgat aaagagtttg
      781 gaataggtga cctcgtgtgg ggaaagatca agggcttctc ctggtggcct gccatggtgg
      841 tgtcctggaa agccacctcc aagcgacagg ccatgcccgg aatgcgctgg gtacagtggt
      901 ttggtgatgg caagttttct gagatctctg ctgacaaact ggtggctctg gggctgttca
      961 gccagcactt taatctggct accttcaata agctggtttc ttataggaag gccatgtacc
//
