LOCUS       sequence-122273-        1076 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for Bxb1-mediated recombination of the GFP-Tet1 cDNA into a 
            MIN-tagged locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Mulholland CB, Smets M, Schmidtmann E, Leidescher S, Markaki Y, 
            Hofweber M, Qin W, Manzo M, Kremmer E, Thanisch K, Bauer C, Rombaut 
            P, Herzog F, Leonhardt H, Bultmann S
  TITLE     A modular open platform for systematic functional studies under 
            physiological conditions.
  JOURNAL   Nucleic Acids Res. 2015 May 24. pii: gkv550.
  PUBMED    26007658
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 24. pii: gkv550."
COMMENT     Sequence Label: attB-GFP-Tet1-Poly(A)
FEATURES             Location/Qualifiers
     source          1..1076
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      72..81
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctctcggcat ggacgagctg tacaagagcg gcctgaggag cagagcccag gcgagcaaca
       61 gcgccgtgga cgccaccatg ggcgatcgca tgtctcggtc ccgccccgca aagccttcca
      121 aatcagtcaa aacaaagcta cagaaaaaga aagacatcca gatgaagacg aagacatcaa
      181 agcaggctgt cagacatggg gcatcagcga aggcagtgaa ccccggaaaa ccgaagcaat
      241 taattaaaag aagagatggt aagaaggaaa cggaagacaa aaccccgaca ccggcgccga
      301 gttttctgac gcgagccggg gccgcccgaa tgaatcggga tcgaaaccaa gttctttttc
      361 agaacccaga ctccttaact tgcaacgggt ttacaatggc tcttcgaaga acctctctta
      421 gctggcgact ctcccagcgc ccagtagtca cacccaaacc taagaaagta ccaccttcaa
      481 agaagcagtg tacacataat atccaggacg agccgggggt aaagcactct gaaaatgatt
      541 cagtccccag ccaacacgct accgtgtccc caggcacaga gaatggtgaa cagaacaggt
      601 gtttagttga aggggagagc caagagataa cccagtcttg ccctgtattt gaggaaagaa
      661 tagaagatac ccaaagctgt atttccgcat ctgggaacct ggaagcagaa atttcttggc
      721 cactggaagg gacacattgt gaagaactac tttctcatca gacatctgat aatgagtgca
      781 cttcccctca agagtgtgct ccattgcccc aaagatcaac ttccgaagtt acctctcaga
      841 aaaacactag caatcagtta gcagacttga gctcacaagt agagtctatc aagctgtctg
      901 atccttctcc gaatcctacg ggaagcgacc ataatggctt tccagactcc agttttagga
      961 tcgttcctga attggacctg aaaacctgca tgcctcttga tgagtctgtg tatcccacag
     1021 ctctgataag attcatcttg gcaggctcac agccggatgt ctttgatact aagccc
//
