LOCUS       sequence-122275-         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  storage vector for BASIC part: GFP(ORF).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Storch M, Casini A, Mackrow B, Fleming T, Trewhitt H, Ellis T, 
            Baldwin GS
  TITLE     BASIC: A New Biopart Assembly Standard for Idempotent Cloning 
            Provides Accurate, Single-Tier DNA Assembly for Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2015 Apr 16.
  PUBMED    25746445
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 16."
COMMENT     Sequence Label: BASIC_12_ATG-GFP-B0015
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             25..738
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      767..838
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gcaagatctg gtgggtctct gtccatgcgt aaaggcgaag aactgttcac gggcgtagtt
       61 ccgattctgg tcgagctgga cggcgatgtg aacggtcata agtttagcgt tcgcggtgaa
      121 ggtgagggcg acgcgaccaa cggcaaactg accctgaagt tcatctgcac caccggtaaa
      181 ctgccggtgc cttggccgac cttggtgacg acgttgacgt atggcgtgca gtgttttgcg
      241 cgttatccgg accacatgaa acaacacgat ttcttcaaat ctgcgatgcc ggagggttac
      301 gtccaggagc gtaccatttc cttcaaggat gatggcactt acaaaactcg cgcagaggtt
      361 aagtttgaag gtgacacgct ggtcaatcgt atcgaattga agggtatcga ctttaaagag
      421 gatggtaaca ttctgggcca taaactggag tataacttca acagccataa tgtttacatt
      481 acggcagaca agcaaaagaa cggcatcaag gccaatttca agattcgcca caatgttgag
      541 gacggtagcg tccaactggc cgaccattac cagcagaaca ccccaattgg tgacggtccg
      601 gttttgctgc cggataatca ctatctgagc acccaaagcg tgctgagcaa agatccgaac
      661 gaaaaacgtg atcacatggt cctgctggaa tttgtgaccg ctgcgggcat cacccacggt
      721 atggacgagc tgtataagcg tccgtaataa tactagagcc aggcatcaaa taaaacgaaa
      781 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
      841 ctagagtcac actggctcac cttcg
//
