LOCUS       sequence-122277-         792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  storage vector for BASIC part: RBS-GFP(ORF).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Storch M, Casini A, Mackrow B, Fleming T, Trewhitt H, Ellis T, 
            Baldwin GS
  TITLE     BASIC: A New Biopart Assembly Standard for Idempotent Cloning 
            Provides Accurate, Single-Tier DNA Assembly for Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2015 Apr 16.
  PUBMED    25746445
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 16."
COMMENT     Sequence Label: BASIC_11_RBS34-GFP-B0015
FEATURES             Location/Qualifiers
     source          1..792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             25..36
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             43..756
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 gcaagatctg gtgggtctct gtccaaagag gagaaatact agatgcgtaa aggcgaagaa
       61 ctgttcacgg gcgtagttcc gattctggtc gagctggacg gcgatgtgaa cggtcataag
      121 tttagcgttc gcggtgaagg tgagggcgac gcgaccaacg gcaaactgac cctgaagttc
      181 atctgcacca ccggtaaact gccggtgcct tggccgacct tggtgacgac gttgacgtat
      241 ggcgtgcagt gttttgcgcg ttatccggac cacatgaaac aacacgattt cttcaaatct
      301 gcgatgccgg agggttacgt ccaggagcgt accatttcct tcaaggatga tggcacttac
      361 aaaactcgcg cagaggttaa gtttgaaggt gacacgctgg tcaatcgtat cgaattgaag
      421 ggtatcgact ttaaagagga tggtaacatt ctgggccata aactggagta taacttcaac
      481 agccataatg tttacattac ggcagacaag caaaagaacg gcatcaaggc caatttcaag
      541 attcgccaca atgttgagga cggtagcgtc caactggccg accattacca gcagaacacc
      601 ccaattggtg acggtccggt tttgctgccg gataatcact atctgagcac ccaaagcgtg
      661 ctgagcaaag atccgaacga aaaacgtgat cacatggtcc tgctggaatt tgtgaccgct
      721 gcgggcatca cccacggtat ggacgagctg tataagcgtc cgtaataata ctagagccag
      781 gcatcaaata aa
//
