LOCUS       sequence-122282-        2189 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes a recombineering cassette for tagging a target gene within a
            genomic clone with a fluorescent protein. Cassette: FRT-galK-FRT in 
            the 2nd intron of mChO (mCherry variant).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Tursun B, Cochella L, Carrera I, Hobert O
  TITLE     A toolkit and robust pipeline for the generation of fosmid-based 
            reporter genes in C. elegans.
  JOURNAL   PLoS One. 2009;4(3):e4625. doi: 10.1371/journal.pone.0004625. Epub 
            2009 Mar 4.
  PUBMED    19259264
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009"; volume: "4(3):e4625. doi"; pages: " 
            10.1371/journal.pone.0004625. Epub 2009 Mar 4"
COMMENT     Sequence Label: pBALU4
FEATURES             Location/Qualifiers
     source          1..2189
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(424..457)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     promoter        461..508
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     primer_bind     complement(1690..1706)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1690..1706)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    complement(1715..1748)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 atggtctcaa agggtgaaga agataacatg gcaattatta aagagtttat gcgtttcaag
       61 gtgcatatgg agggatctgt caatgggcat gagtttgaaa ttgaaggtga aggagaaggc
      121 cgaccatatg agggaacaca aaccgcaaaa ctaaaggtaa gtttaaacat atatatacta
      181 actaaccctg attatttaaa ttttcaggta actaaaggcg gaccattacc attcgcctgg
      241 gacatcctct ctccacagtt catgtatgga agtaaagctt atgttaaaca tccggcagat
      301 ataccagatt atttgaaact ttcattcccg gagggtttta agtgggaacg cgtaatgaat
      361 tttgaagacg gaggagttgt tacagtgacg caagactcaa ggtaagttta aacagttcgg
      421 tacgaagttc ctatactttc tagagaatag gaacttccct gttgacaatt aatcatcggc
      481 atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaacccagg aggcagatca
      541 tgagtctgaa agaaaaaaca caatctctgt ttgccaacgc atttggctac cctgccactc
      601 acaccattca ggcgcctggc cgcgtgaatt tgattggtga acacaccgac tacaacgacg
      661 gtttcgttct gccctgcgcg attgattatc aaaccgtgat cagttgtgca ccacgcgatg
      721 accgtaaagt tcgcgtgatg gcagccgatt atgaaaatca gctcgacgag ttttccctcg
      781 atgcgcccat tgtcgcacat gaaaactatc aatgggctaa ctacgttcgt ggcgtggtga
      841 aacatctgca actgcgtaac aacagcttcg gcggcgtgga catggtgatc agcggcaatg
      901 tgccgcaggg tgccgggtta agttcttccg cttcactgga agtcgcggtc ggaaccgtat
      961 tgcagcagct ttatcatctg ccgctggacg gcgcacaaat cgcgcttaac ggtcaggaag
     1021 cagaaaacca gtttgtaggc tgtaactgcg ggatcatgga tcagctaatt tccgcgctcg
     1081 gcaagaaaga tcatgccttg ctgatcgatt gccgctcact ggggaccaaa gcagtttcca
     1141 tgcccaaagg tgtggctgtc gtcatcatca acagtaactt caaacgtacc ctggttggca
     1201 gcgaatacaa cacccgtcgt gaacagtgcg aaaccggtgc gcgtttcttc cagcagccag
     1261 ccctgcgtga tgtcaccatt gaagagttca acgctgttgc gcatgaactg gacccgatcg
     1321 tggcaaaacg cgtgcgtcat atactgactg aaaacgcccg caccgttgaa gctgccagcg
     1381 cgctggagca aggcgacctg aaacgtatgg gcgagttgat ggcggagtct catgcctcta
     1441 tgcgcgatga tttcgaaatc accgtgccgc aaattgacac tctggtagaa atcgtcaaag
     1501 ctgtgattgg cgacaaaggt ggcgtacgca tgaccggcgg cggatttggc ggctgtatcg
     1561 tcgcgctgat cccggaagag ctggtgcctg ccgtacagca agctgtcgct gaacaatatg
     1621 aagcaaaaac aggtattaaa gagacttttt acgtttgtaa accatcacaa ggagcaggac
     1681 agtgctgagg atccactagt tctagagcgg ccgcgaagtt cctatacttt ctagagaata
     1741 ggaacttcta actaaccata catatttaaa ttttcagcct ccaagatgga gaatttattt
     1801 ataaagtcaa acttcgagga acgaatttcc cctcggatgg acctgttatg cagaagaaga
     1861 ctatgggatg ggaagcttca agtgaaagaa tgtaccctga agacggtgct cttaagggag
     1921 agattaaaca acgtcttaaa ttgaaagatg gaggacatta cgatgctgag gtaagtttaa
     1981 acatgatttt actaactaac taatctgatt taaattttca ggtgaagaca acttacaaag
     2041 ccaaaaaacc agttcagctg ccaggagcgt acaatgttaa tattaaactg gatatcacct
     2101 cccacaacga ggattacact atcgttgagc aatatgaaag agctgaaggg cggcactcga
     2161 caggtggcat ggatgaattg tataagtaa
//
