LOCUS       sequence-122284-        2044 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes a recombineering cassette for tagging a target gene within a
            genomic clone with a fluorescent protein. Cassette: Venus in frame 
            w/ FRT-galK-FRT; no introns; N-terminal fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Tursun B, Cochella L, Carrera I, Hobert O
  TITLE     A toolkit and robust pipeline for the generation of fosmid-based 
            reporter genes in C. elegans.
  JOURNAL   PLoS One. 2009;4(3):e4625. doi: 10.1371/journal.pone.0004625. Epub 
            2009 Mar 4.
  PUBMED    19259264
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009"; volume: "4(3):e4625. doi"; pages: " 
            10.1371/journal.pone.0004625. Epub 2009 Mar 4"
COMMENT     Sequence Label: pBALU6
FEATURES             Location/Qualifiers
     source          1..2044
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1..3,4..6,7..717)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(46..67)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(307..326)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     654..675
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(720..753)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     promoter        757..804
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     primer_bind     complement(1986..2002)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1986..2002)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    complement(2011..2044)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagct gatctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccctgggcta cggcctgcag tgcttcgccc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaactacaa cagccacaac gtctatatca ccgccgacaa gcagaagaac
      481 ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcggcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      601 tacctgagct accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      661 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagggg
      721 aagttcctat actttctaga gaataggaac ttccctgttg acaattaatc atcggcatag
      781 tatatcggca tagtataata cgacaaggtg aggaactaaa cccaggaggc agatcatgag
      841 tctgaaagaa aaaacacaat ctctgtttgc caacgcattt ggctaccctg ccactcacac
      901 cattcaggcg cctggccgcg tgaatttgat tggtgaacac accgactaca acgacggttt
      961 cgttctgccc tgcgcgattg attatcaaac cgtgatcagt tgtgcaccac gcgatgaccg
     1021 taaagttcgc gtgatggcag ccgattatga aaatcagctc gacgagtttt ccctcgatgc
     1081 gcccattgtc gcacatgaaa actatcaatg ggctaactac gttcgtggcg tggtgaaaca
     1141 tctgcaactg cgtaacaaca gcttcggcgg cgtggacatg gtgatcagcg gcaatgtgcc
     1201 gcagggtgcc gggttaagtt cttccgcttc actggaagtc gcggtcggaa ccgtattgca
     1261 gcagctttat catctgccgc tggacggcgc acaaatcgcg cttaacggtc aggaagcaga
     1321 aaaccagttt gtaggctgta actgcgggat catggatcag ctaatttccg cgctcggcaa
     1381 gaaagatcat gccttgctga tcgattgccg ctcactgggg accaaagcag tttccatgcc
     1441 caaaggtgtg gctgtcgtca tcatcaacag taacttcaaa cgtaccctgg ttggcagcga
     1501 atacaacacc cgtcgtgaac agtgcgaaac cggtgcgcgt ttcttccagc agccagccct
     1561 gcgtgatgtc accattgaag agttcaacgc tgttgcgcat gaactggacc cgatcgtggc
     1621 aaaacgcgtg cgtcatatac tgactgaaaa cgcccgcacc gttgaagctg ccagcgcgct
     1681 ggagcaaggc gacctgaaac gtatgggcga gttgatggcg gagtctcatg cctctatgcg
     1741 cgatgatttc gaaatcaccg tgccgcaaat tgacactctg gtagaaatcg tcaaagctgt
     1801 gattggcgac aaaggtggcg tacgcatgac cggcggcgga tttggcggct gtatcgtcgc
     1861 gctgatcccg gaagagctgg tgcctgccgt acagcaagct gtcgctgaac aatatgaagc
     1921 aaaaacaggt attaaagaga ctttttacgt ttgtaaacca tcacaaggag caggacagtg
     1981 ctgaggatcc actagttcta gagcggccgc gaagttccta tactttctag agaataggaa
     2041 cttc
//
