LOCUS       sequence-122287-        2482 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes a recombineering cassette for tagging a target gene within a
            genomic clone with a fluorescent protein. Cassette: bicistronic; 
            FRT-galK-FRT in 2nd GFP intron;use C-term after STOP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Tursun B, Cochella L, Carrera I, Hobert O
  TITLE     A toolkit and robust pipeline for the generation of fosmid-based 
            reporter genes in C. elegans.
  JOURNAL   PLoS One. 2009;4(3):e4625. doi: 10.1371/journal.pone.0004625. Epub 
            2009 Mar 4.
  PUBMED    19259264
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009"; volume: "4(3):e4625. doi"; pages: " 
            10.1371/journal.pone.0004625. Epub 2009 Mar 4"
COMMENT     Sequence Label: pBALU9
FEATURES             Location/Qualifiers
     source          1..2482
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             255..275
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(318..346)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     protein_bind    complement(686..719)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     promoter        723..770
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     primer_bind     complement(1952..1968)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1952..1968)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    complement(1977..2010)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     2419..2439
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 gctgtctcat cctactttca cctagttaac tgcttgtctt aaaatctatg cttctcttta
       61 gtatctaaaa ttttcctaga agcttacaag tatataaatg gtctcttctc aataaaggtt
      121 gtatatttat tcatcttatt gaatctgcca tttcctcgtt tttgcgagtt tatatacctt
      181 ccaattttct ttctattgta ttttcaactt ctaattttaa ttcagggaaa ctgcttcaac
      241 gcatcatgac cgctccaaag aagaaacgca aagtaccggt agaaaaaatg agtaaaggag
      301 aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc
      361 acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta
      421 aatttatttg cactactgga aaactacctg ttccatgggt aagtttaaac atatatatac
      481 taactaaccc tgattattta aattttcagc caacacttgt cactactttc tgttatggtg
      541 ttcaatgctt ctcgagatac ccagatcata tgaaacggca tgactttttc aagagtgcca
      601 tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg aactacaaga
      661 cacgtaagtt taaacagttc ggtacgaagt tcctatactt tctagagaat aggaacttcc
      721 ctgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga
      781 actaaaccca ggaggcagat catgagtctg aaagaaaaaa cacaatctct gtttgccaac
      841 gcatttggct accctgccac tcacaccatt caggcgcctg gccgcgtgaa tttgattggt
      901 gaacacaccg actacaacga cggtttcgtt ctgccctgcg cgattgatta tcaaaccgtg
      961 atcagttgtg caccacgcga tgaccgtaaa gttcgcgtga tggcagccga ttatgaaaat
     1021 cagctcgacg agttttccct cgatgcgccc attgtcgcac atgaaaacta tcaatgggct
     1081 aactacgttc gtggcgtggt gaaacatctg caactgcgta acaacagctt cggcggcgtg
     1141 gacatggtga tcagcggcaa tgtgccgcag ggtgccgggt taagttcttc cgcttcactg
     1201 gaagtcgcgg tcggaaccgt attgcagcag ctttatcatc tgccgctgga cggcgcacaa
     1261 atcgcgctta acggtcagga agcagaaaac cagtttgtag gctgtaactg cgggatcatg
     1321 gatcagctaa tttccgcgct cggcaagaaa gatcatgcct tgctgatcga ttgccgctca
     1381 ctggggacca aagcagtttc catgcccaaa ggtgtggctg tcgtcatcat caacagtaac
     1441 ttcaaacgta ccctggttgg cagcgaatac aacacccgtc gtgaacagtg cgaaaccggt
     1501 gcgcgtttct tccagcagcc agccctgcgt gatgtcacca ttgaagagtt caacgctgtt
     1561 gcgcatgaac tggacccgat cgtggcaaaa cgcgtgcgtc atatactgac tgaaaacgcc
     1621 cgcaccgttg aagctgccag cgcgctggag caaggcgacc tgaaacgtat gggcgagttg
     1681 atggcggagt ctcatgcctc tatgcgcgat gatttcgaaa tcaccgtgcc gcaaattgac
     1741 actctggtag aaatcgtcaa agctgtgatt ggcgacaaag gtggcgtacg catgaccggc
     1801 ggcggatttg gcggctgtat cgtcgcgctg atcccggaag agctggtgcc tgccgtacag
     1861 caagctgtcg ctgaacaata tgaagcaaaa acaggtatta aagagacttt ttacgtttgt
     1921 aaaccatcac aaggagcagg acagtgctga ggatccacta gttctagagc ggccgcgaag
     1981 ttcctatact ttctagagaa taggaacttc taactaacca tacatattta aattttcagg
     2041 tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa aaggtattga
     2101 ttttaaagaa gatggaaaca ttcttggaca caaattggaa tacaactata actcacacaa
     2161 tgtatacatc atggcagaca aacaaaagaa tggaatcaaa gttgtaagtt taaacatgat
     2221 tttactaact aactaatctg atttaaattt tcagaacttc aaaattagac acaacattga
     2281 agatggaagc gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc
     2341 tgtcctttta ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa
     2401 cgaaaagaga gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg
     2461 catggatgaa ctatacaaat ag
//
