LOCUS       sequence-122297-        2438 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes a recombineering cassette for tagging a target gene within a
            genomic clone with a fluorescent protein. Cassette: bicistronic; 
            FRT-galK-FRT in 2ndmChO intron; use C-term after STOP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Tursun B, Cochella L, Carrera I, Hobert O
  TITLE     A toolkit and robust pipeline for the generation of fosmid-based 
            reporter genes in C. elegans.
  JOURNAL   PLoS One. 2009;4(3):e4625. doi: 10.1371/journal.pone.0004625. Epub 
            2009 Mar 4.
  PUBMED    19259264
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009"; volume: "4(3):e4625. doi"; pages: " 
            10.1371/journal.pone.0004625. Epub 2009 Mar 4"
COMMENT     Sequence Label: pBALU18
FEATURES             Location/Qualifiers
     source          1..2438
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             217..237
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(673..706)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     promoter        710..757
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     primer_bind     complement(1939..1955)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1939..1955)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    complement(1964..1997)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 gccatgttgt taccttgtat taaaacaatg tttcaagttg aatttgttct tgttgttgaa
       61 ttctattcac atggtcttta cccatgcttc atatcttatt gttttcttac cctggtcact
      121 ttgtggcatt aatactttct ttcagcgttc tatcaaacgt ttgttctctt tggagctaag
      181 gcaaaccgcg caacttacga ttgaagaatg accgctccaa agaagaaacg caaagtaccg
      241 gtagaaaaaa tggtctcaaa gggtgaagaa gataacatgg caattattaa agagtttatg
      301 cgtttcaagg tgcatatgga gggatctgtc aatgggcatg agtttgaaat tgaaggtgaa
      361 ggagaaggcc gaccatatga gggaacacaa accgcaaaac taaaggtaag tttaaacata
      421 tatatactaa ctaaccctga ttatttaaat tttcaggtaa ctaaaggcgg accattacca
      481 ttcgcctggg acatcctctc tccacagttc atgtatggaa gtaaagctta tgttaaacat
      541 ccggcagata taccagatta tttgaaactt tcattcccgg agggttttaa gtgggaacgc
      601 gtaatgaatt ttgaagacgg aggagttgtt acagtgacgc aagactcaag gtaagtttaa
      661 acagttcggt acgaagttcc tatactttct agagaatagg aacttccctg ttgacaatta
      721 atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact aaacccagga
      781 ggcagatcat gagtctgaaa gaaaaaacac aatctctgtt tgccaacgca tttggctacc
      841 ctgccactca caccattcag gcgcctggcc gcgtgaattt gattggtgaa cacaccgact
      901 acaacgacgg tttcgttctg ccctgcgcga ttgattatca aaccgtgatc agttgtgcac
      961 cacgcgatga ccgtaaagtt cgcgtgatgg cagccgatta tgaaaatcag ctcgacgagt
     1021 tttccctcga tgcgcccatt gtcgcacatg aaaactatca atgggctaac tacgttcgtg
     1081 gcgtggtgaa acatctgcaa ctgcgtaaca acagcttcgg cggcgtggac atggtgatca
     1141 gcggcaatgt gccgcagggt gccgggttaa gttcttccgc ttcactggaa gtcgcggtcg
     1201 gaaccgtatt gcagcagctt tatcatctgc cgctggacgg cgcacaaatc gcgcttaacg
     1261 gtcaggaagc agaaaaccag tttgtaggct gtaactgcgg gatcatggat cagctaattt
     1321 ccgcgctcgg caagaaagat catgccttgc tgatcgattg ccgctcactg gggaccaaag
     1381 cagtttccat gcccaaaggt gtggctgtcg tcatcatcaa cagtaacttc aaacgtaccc
     1441 tggttggcag cgaatacaac acccgtcgtg aacagtgcga aaccggtgcg cgtttcttcc
     1501 agcagccagc cctgcgtgat gtcaccattg aagagttcaa cgctgttgcg catgaactgg
     1561 acccgatcgt ggcaaaacgc gtgcgtcata tactgactga aaacgcccgc accgttgaag
     1621 ctgccagcgc gctggagcaa ggcgacctga aacgtatggg cgagttgatg gcggagtctc
     1681 atgcctctat gcgcgatgat ttcgaaatca ccgtgccgca aattgacact ctggtagaaa
     1741 tcgtcaaagc tgtgattggc gacaaaggtg gcgtacgcat gaccggcggc ggatttggcg
     1801 gctgtatcgt cgcgctgatc ccggaagagc tggtgcctgc cgtacagcaa gctgtcgctg
     1861 aacaatatga agcaaaaaca ggtattaaag agacttttta cgtttgtaaa ccatcacaag
     1921 gagcaggaca gtgctgagga tccactagtt ctagagcggc cgcgaagttc ctatactttc
     1981 tagagaatag gaacttctaa ctaaccatac atatttaaat tttcagcctc caagatggag
     2041 aatttattta taaagtcaaa cttcgaggaa cgaatttccc ctcggatgga cctgttatgc
     2101 agaagaagac tatgggatgg gaagcttcaa gtgaaagaat gtaccctgaa gacggtgctc
     2161 ttaagggaga gattaaacaa cgtcttaaat tgaaagatgg aggacattac gatgctgagg
     2221 taagtttaaa catgatttta ctaactaact aatctgattt aaattttcag gtgaagacaa
     2281 cttacaaagc caaaaaacca gttcagctgc caggagcgta caatgttaat attaaactgg
     2341 atatcacctc ccacaacgag gattacacta tcgttgagca atatgaaaga gctgaagggc
     2401 ggcactcgac aggtggcatg gatgaattgt ataagtaa
//
