LOCUS       sequence-12230-e        2247 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Gavet O, Pines J
  TITLE     Progressive activation of CyclinB1-Cdk1 coordinates entry to 
            mitosis.
  JOURNAL   Dev Cell. 2010 Apr 20. 18(4):533-43.
  PUBMED    20412769
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2010 Apr 20. 18(4):533-43."
COMMENT     Sequence Label: cdk1 FRET sensor (2327)
FEATURES             Location/Qualifiers
     source          1..2247
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(16..18,19..21,22..732)
                     /codon_start=1
                     /product="yellow fluorescent protein optimized for FRET 
                     with CyPet (Nguyen and Daugherty, 2005)"
                     /label=YPet
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLL
                     EFLTAAGITEGMNELYK"
     CDS             join(1498..1500,1501..1503,1504..2214)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     2151..2172
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2215..2238
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gctagcgcta ccggtatggt gagcaaaggc gaagagctgt tcaccggcgt ggtgcccatc
       61 ctggtggagc tggacggcga cgtgaacggc cacaagttca gcgtgagcgg cgagggcgag
      121 ggcgacgcca cctacggcaa gctgaccctg aagctgctgt gcaccaccgg caagctgccc
      181 gtgccctggc ccaccctggt gaccaccctg ggctacggcg tgcagtgctt cgcccggtac
      241 cccgaccaca tgaagcagca cgacttcttc aagagcgcca tgcccgaggg ctacgtgcag
      301 gagcggacca tcttcttcaa ggacgacggc aactacaaga cccgggccga ggtgaagttc
      361 gagggcgaca ccctggtgaa ccggatcgag ctgaagggca tcgacttcaa ggaggacggc
      421 aacatcctgg gccacaagct ggagtacaac tacaacagcc acaacgtgta catcaccgcc
      481 gacaagcaga agaacggcat caaggccaac ttcaagatcc ggcacaacat cgaggacggc
      541 ggcgtgcagc tggccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
      601 ctgcccgaca accactacct gagctaccag agcgccctgt tcaaggaccc caacgagaag
      661 cgggaccaca tggtgctgct ggagttcctg accgccgccg gcatcaccga gggcatgaac
      721 gagctctata agagatctca cctcagtgac atgctgcagc agctgcacag tgtcaatgcc
      781 tccaagccct cggagcgtgg gctggtcagg caagaggagg ctgaggatcc tgcctgcatc
      841 cccatcttct gggtcagcaa gtgggtggac tattcggaca agtacggcct tgggtatcag
      901 ctctgtgata acagcgtggg ggtgctcttc aatgactcaa cacgcctcat cctctacaat
      961 gatggtgaca gcctgcagta catagagcgt gacggcactg agtcctacct caccgtgagt
     1021 tcccatccca actccttgat gaagaagatc accctcctta aatatttccg caattacatg
     1081 agcgagcact tgctgaaggc aggtgccaac atcacgccgc gcgaaggtga tgagctcgcc
     1141 cggctgccct acctacggac ctggttccgc acccgcagcg ccatcatcct gcacctcagc
     1201 aacggcagcg tgcagatcaa cttcttccag gatcacacca agctcatctt gtgcccactg
     1261 atggcagccg tgacctacat cgacgagaag cgggacttcc gcacataccg cctgagtctc
     1321 ctggaggagt acggctgctg caaggagctg gccagccggc tccgctacgc ccgcactatg
     1381 gtggacaagc tgctgagcgg cggcacaggc ggcacaggcg gcacaggcgg cacaggcggt
     1441 acccctgagc ctattttggt tgatacttcc tctccaagcc caatggaaac actcgagatg
     1501 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     1561 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     1621 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     1681 gtgaccaccc tgacctgggg cgtgcagtgc ttcgcccgct accccgacca catgaagcag
     1741 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     1801 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     1861 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     1921 ctggagtaca acgccatcag cgacaacgtc tatatcaccg ccgacaagca gaagaacggc
     1981 atcaaggcca acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     2041 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     2101 ctgagcaccc agtccaagct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     2161 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caaggattac
     2221 aaggatgacg acgataagta aggatcc
//
