LOCUS       sequence-122302-        1150 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  It allows to extend the genomic insert in a fosmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Tursun B, Cochella L, Carrera I, Hobert O
  TITLE     A toolkit and robust pipeline for the generation of fosmid-based 
            reporter genes in C. elegans.
  JOURNAL   PLoS One. 2009;4(3):e4625. doi: 10.1371/journal.pone.0004625. Epub 
            2009 Mar 4.
  PUBMED    19259264
REFERENCE   2  (bases 1 to 1150)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009"; volume: "4(3):e4625. doi"; pages: " 
            10.1371/journal.pone.0004625. Epub 2009 Mar 4"
COMMENT     Sequence Label: pBALU-ext
FEATURES             Location/Qualifiers
     source          1..1150
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     12..34
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     26..43
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     27..43
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             239..1033
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(293..312)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     903..922
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(1132..1148)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1132..1148)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattgt aatacgactc
       61 actatagggc gaattcgagc tcggtacccg gggatcccac ccggaattgc cagctggggc
      121 gccctctggt aaggttggga agccctgcaa agtaaactgg atggctttct tgccgccaag
      181 gatctgatgg cgcaggggat caagatctga tcaagagaca ggatgaggat cgtttcgcat
      241 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
      301 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
      361 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
      421 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
      481 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
      541 tctcctgtca tcccaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
      601 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
      661 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
      721 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
      781 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
      841 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
      901 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
      961 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     1021 cgagttcttc tgaattgaaa aaggaagagt gtgggatcct ctagagtcga cctgcaggca
     1081 tgcaagcttg agtattctat agtctcacct aaatagcttg gcgtaatcat ggtcatagct
     1141 gtttcctgtg
//
