LOCUS       sequence-122315-        1157 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for Bxb1-mediated recombination of the mCh-Dnmt3b1 cDNA into
            a MIN-tagged locus using Neomycin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Mulholland CB, Smets M, Schmidtmann E, Leidescher S, Markaki Y, 
            Hofweber M, Qin W, Manzo M, Kremmer E, Thanisch K, Bauer C, Rombaut 
            P, Herzog F, Leonhardt H, Bultmann S
  TITLE     A modular open platform for systematic functional studies under 
            physiological conditions.
  JOURNAL   Nucleic Acids Res. 2015 May 24. pii: gkv550.
  PUBMED    26007658
REFERENCE   2  (bases 1 to 1157)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 24. pii: gkv550."
COMMENT     Sequence Label: attB-mCh-Dnmt3b1-Poly(A)-NeoR
FEATURES             Location/Qualifiers
     source          1..1157
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      307..316
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             396..407
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
       61 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
      121 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
      181 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
      241 ggcatggacg agctgtacaa gagcggcctg aggagcagag cccaggcgag caacagcgcc
      301 gtggacgcca ccatgggcga tcgcatgaag ggagacagca gacatctgaa tgaagaagag
      361 ggtgccagcg ggtatgagga gtgcattatc gttaatggga acttcagtga ccagtcctca
      421 gacacgaagg atgctccctc acccccagtc ttggaggcaa tctgcacaga gccagtctgc
      481 acaccagaga ccagaggccg caggtcaagc tcccggctgt ctaagaggga ggtctccagc
      541 cttctgaatt acacgcagga catgacagga gatggagaca gagatgatga agtagatgat
      601 gggaatggct ctgatattct aatgccaaag ctcacccgtg agaccaagga caccaggacg
      661 cgctctgaaa gcccggctgt ccgaacccga catagcaatg ggacctccag cttggagagg
      721 caaagagcct cccccagaat cacccgaggt cggcagggcc gccaccatgt gcaggagtac
      781 cctgtggagt ttccggctac caggtctcgg agacgtcgag catcgtcttc agcaagcacg
      841 ccatggtcat cccctgccag cgtcgacttc atggaagaag tgacacctaa gagcgtcagt
      901 accccatcag ttgacttgag ccaggatgga gatcaggagg gtatggatac cacacaggtg
      961 gatgcagaga gcagagatgg agacagcaca gagtatcagg atgataaaga gtttggaata
     1021 ggtgacctcg tgtggggaaa gatcaagggc ttctcctggt ggcctgccat ggtggtgtcc
     1081 tggaaagcca cctccaagcg acaggccatg cccggaatgc gctgggtaca gtggtttggt
     1141 gatggcaagt tttctga
//
