LOCUS       sequence-122327-         704 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  px330 with gRNA towards P53 exon 8. Cas9 is expressed from a CAG 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 704)
  TITLE     Cas9/CRISPR pig transposon 
            plasmid_LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: px330_P53 exon 8 gRNA
FEATURES             Location/Qualifiers
     source          1..704
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     116..135
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        214..289
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        386..671
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 agagataatt ggaattaatt tgactgtaaa cacaaagata ttagtacaaa atacgtgacg
       61 tagaaagtaa taatttcttg ggtagtttgc agttttaaaa ttatgtttta aaatggacta
      121 tcatatgctt accgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa
      181 aggacgaaac accgtgcgtg tttgtgcctg tccgttttag agctagaaat agcaagttaa
      241 aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct tttttgtttt
      301 agagctagaa atagcaagtt aaaataaggc tagtccgttt ttagcgcgtg cgccaattct
      361 gcagacaaat ggctctagag gtacccgtta cataacttac ggtaaatggc ccgcctggct
      421 gaccgcccaa cgacccccgc ccattgacgt caatagtaac gccaataggg actttccatt
      481 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
      541 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattgtg
      601 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      661 ctattaccat ggtcgaggtg agccccacgt tctgcttcac tctc
//
