LOCUS       sequence-122333-         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mCherry tagged Geph for mammalian expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Tyagarajan SK, Ghosh H, Yevenes GE, Nikonenko I, Ebeling C, 
            Schwerdel C, Sidler C, Zeilhofer HU, Gerrits B, Muller D, Fritschy 
            JM
  TITLE     Regulation of GABAergic synapse formation and plasticity by 
            GSK3beta-dependent phosphorylation of gephyrin.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 4;108(1):379-84. doi: 
            10.1073/pnas.1011824108. Epub 2010 Dec 20.
  PUBMED    21173228
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1011824108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-4- 4"; volume: "108"; issue: "1"; pages: "379-84"
COMMENT     Sequence Label: pmCherryC2-Gephyrin P1
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             23..730
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(168..186)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     436..455
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gtgaaccgtc agatccgcta gcatggtgag caagggcgag gaggataaca tggccatcat
       61 caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga
      121 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
      181 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg
      241 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc
      301 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
      361 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa
      421 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga
      481 gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa
      541 ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca
      601 gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta
      661 caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga
      721 gctgtacaag ggcgctatct cgagctcaag cttcgaattc atggcgaccg agggaatgat
      781 cctcacaaac cacgaccatc aaatccgtgt cggagtcctc acagtgagtg atagctgctt
      841 caggaatctt gca
//
