LOCUS       sequence-122334-         773 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  storage vector for BASIC part: Kan-MB1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Storch M, Casini A, Mackrow B, Fleming T, Trewhitt H, Ellis T, 
            Baldwin GS
  TITLE     BASIC: A New Biopart Assembly Standard for Idempotent Cloning 
            Provides Accurate, Single-Tier DNA Assembly for Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2015 Apr 16.
  PUBMED    25746445
REFERENCE   2  (bases 1 to 773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 16."
COMMENT     Sequence Label: BASIC_2_Kan-MB1
FEATURES             Location/Qualifiers
     source          1..773
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     692..711
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agacgtttcc cgttgaatat ggctcataac accacttgta ttactgttta tgtaagcaga
       61 cagttttatt gttcatgatg atatattttt atcttgtgca atgtaacatc agagattttg
      121 agacacaacg tggctttgtt gaataaatcg aacggacaga gacccaccag acgataggtc
      181 tcccgagcct caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
      241 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
      301 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
      361 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
      421 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
      481 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
      541 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
      601 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
      661 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
      721 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggg
//
