LOCUS       sequence-122340-         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  receiving vector of ORFs for the construction of standard ORF parts 
            in YeastFab work.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Guo Y, Dong J, Zhou T, Auxillos J, Li T, Zhang W, Wang L, Shen Y, 
            Luo Y, Zheng Y, Lin J, Chen GQ, Wu Q, Cai Y, Dai J
  TITLE     YeastFab: the design and construction of standard biological parts 
            for metabolic engineering in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2015 May 8. pii: gkv464.
  PUBMED    25956650
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 8. pii: gkv464."
COMMENT     Sequence Label: HcKan_O
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             13..24
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             31..708
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      753..824
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cacatactag agaaagagga gaaatactag atggcttcct ccgaagacgt tatcaaagag
       61 ttcatgcgtt tcaaagttcg tatggaaggt tccgttaacg gtcacgagtt cgaaatcgaa
      121 ggtgaaggtg aaggtcgtcc gtacgaaggt acccagaccg ctaaactgaa agttaccaaa
      181 ggtggtccgc tgccgttcgc ttgggacatc ctgtccccgc agttccagta cggttccaaa
      241 gcttacgtta aacacccggc tgacatcccg gactacctga aactgtcctt cccggaaggt
      301 ttcaaatggg aacgtgttat gaacttcgaa gacggtggtg ttgttaccgt tacccaggac
      361 tcctccctgc aagacggtga gttcatctac aaagttaaac tgcgtggtac caacttcccg
      421 tccgacggtc cggttatgca gaaaaaaacc atgggttggg aagcttccac cgaacgtatg
      481 tacccggaag acggtgctct gaaaggtgaa atcaaaatgc gtctgaaact gaaagacggt
      541 ggtcactacg acgctgaagt taaaaccacc tacatggcta aaaaaccggt tcagctgccg
      601 ggtgcttaca aaaccgacat caaactggac atcacctccc acaacgaaga ctacaccatc
      661 gttgaacagt acgaacgtgc tgaaggtcgt cactccaccg gtgcttaata acgctgatag
      721 tgctagtgta gatcgctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
      781 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
      841 gctcaccttc g
//
