LOCUS       sequence-122343-         639 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Geph G domain expression in mamalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Tyagarajan SK, Ghosh H, Yevenes GE, Imanishi SY, Zeilhofer HU, 
            Gerrits B, Fritschy JM
  TITLE     Extracellular signal-regulated kinase and glycogen synthase kinase 
            3beta regulate gephyrin postsynaptic aggregation and GABAergic 
            synaptic function in a calpain-dependent mechanism.
  JOURNAL   J Biol Chem. 2013 Apr 5;288(14):9634-47. doi: 
            10.1074/jbc.M112.442616. Epub 2013 Feb 13.
  PUBMED    23408424
REFERENCE   2  (bases 1 to 639)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.442616"; journalName: "J Biol Chem"; date: 
            "2013-04-5- 5"; volume: "288"; issue: "14"; pages: "9634-47"
COMMENT     Sequence Label: pCR3-FLAG-Gephyrin G
FEATURES             Location/Qualifiers
     source          1..639
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..51
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        32..50
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             67..114
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
ORIGIN
        1 gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag
       61 cttatggact acaaggacga cgacgacaag gactacaagg acgacgacga caagggagca
      121 ggagcgaccg agggaatgat cctcacaaac cacgaccatc aaatccgtgt cggagtcctc
      181 acagtgagtg atagctgctt caggaatctt gcagaagacc gcagtgggat aaatctaaaa
      241 gatctcgtcc aggatccttc tttgttgggt gggactatat cagcatacaa gatagtacca
      301 gatgaaatag aagaaatcaa ggaaacactc atagactggt gtgatgagaa ggaacttaat
      361 ttgatattaa caactggcgg aacagggttt gcaccacgag atgtcactcc agaggccaca
      421 aaagaagtaa tagaacggga agcgccaggg atggccctgg caatgctgat gggatcactt
      481 aatgttacac ctctgggcat gctctctaga ccagtgtgtg gaataagagg gaaaactctg
      541 ataattaacc tgcctggtag caagaaagga tctcaggaat gctttcagtt catactgcca
      601 gctctacctc atgccatttg aggtaccgag ctcggatcc
//
