LOCUS       sequence-122350-        1121 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  zwf targeting guide RNA E. coli , Low Phosphate Induction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1121)
  TITLE     SUPERSEDE
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1121)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCASCADE-zwf
FEATURES             Location/Qualifiers
     source          1..1121
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     179..196
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(588..615)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(631..702)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(787..806)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 gccaccactg atttgagcgt cagatttcgt gatgcttgtc aggggggcgg agcctatgga
       61 aaaacggctt tgccgcggcc ctctcacttc cctgttaagt atcttcctgg catcttccag
      121 gaaatctccg ccccgttcgt aagccatttc cgctcgccgc agtcgaacga ccgagcgtag
      181 cgagtcagtg agcgaggaag cggaatatat ccctaggaaa aaaaaccccg ccctgtcagg
      241 ggcggggttt ttttttcggt ttatccccgc tggcgcgggg aactcgatct tacggtgcac
      301 tgtactgctt ttacgagcgg tttatccccg ctggcgcggg gaactcgagg tggtaccaga
      361 tcttttgaat tcaaagttaa acaaaattat ttgtagaggg aaaccgttgt ggtctccctg
      421 aatatacgcg ttttatgctc gcggggtaac atgacatgct cgaattacag aaaaataact
      481 tttttgttac atttgtaaga tagtaaggtg tcagaaatag ttatccagca accactcata
      541 gacctagggc agcagatagg gacgacgtgg tgttagctgt gtataaacgc agaaaggccc
      601 acccgaaggt gagccagtgt gactctagta gagagcgttc accgacaaac aacagataaa
      661 acgaaaggcc cagtctttcg actgagcctt tcgttttatt tgatgcctgg atgatgtcta
      721 gattagataa aagtaaagtg attaacagcg cattagagct gcttaatgag gtcggaatcg
      781 aaggtttaac aacccgtaaa ctcgcccaga agctaggtgt agagcagcct acattgtatt
      841 ggcatgtaaa aaataagcgg gctttgctcg acgccttagc cattgagatg ttagataggc
      901 accatactca cttttgccct ttagaagggg aaagctggca agatttttta cgtaataacg
      961 ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg agcaaaagta catttaggta
     1021 cacggcctac agaaaaacag tatgaaactc tcgaaaatca attagccttt ttatgccaac
     1081 aaggtttttc actagagaat gcattatatg cactcagcgc t
//
