LOCUS       sequence-122359-       13373 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13373)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 13373)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB332
FEATURES             Location/Qualifiers
     source          1..13373
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1532..1656
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1693..1698,1699..1716,1717..1723)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1777..2436
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1843..1862)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2778..3083
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2962..2981
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3124..3248)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3280..3532
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3566..3582)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3566..3582)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3579..3601)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3590..3606
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3614..3620,3621..3638,3639..3644))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3659..3680
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    3765..3940
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(6024..6700)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(6067..6087)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    6895..6919
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7440..8231
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8477..9065
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8966..8985
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9219..9236
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9251..9391
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9317..9336)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9477..9499
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9735..9929
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9995..11068)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11497..12126)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgggt ctagaccagc cattatagca actatgttaa aagtgacagt tttctctaat
      361 cgggaatctt gtccgtattg aaagttccct gttcagtgac tgagccgctt aataatattt
      421 tcagtttcga gtacgaggga gagattgaac aaattttggg gtttcagggc taaattctgc
      481 aggggcttta gttttgagtc gggtgaaact ttcctaagtc ttggcaatga actcacattc
      541 gccattgtac ctattaaatg acccaaacat ccttgacaac aaattgacaa gtacgggaac
      601 atgatcttca atatgaaatt taatatgtcc tttatgttgg ctttcctcat caatgattgg
      661 aaactcacat acaaagaaaa gcgtgatata ataaatcact agaatcttct ccgctttcta
      721 agacagtaat gatggatcca cgtgagtgtg aaacatcaat atcaacatcg gtaggagtag
      781 agaagtttct aaatcagaaa aagtgaatag acaagacaac ttactccttg aacgagaata
      841 ttcctcgaga aagagggaaa agagtaaaaa gagtaaaaag ccatggtaca gaatagccca
      901 ttccatggtt attgcagtca aaccgctacg ccagcaaaaa cggaacgaaa ctaaacccgt
      961 tccacaaatt gcgaaagatg gaatgtattc gccatgtata tttcgagatt attatgagac
     1021 ttccggtccc gggaagatgg cctgggttca ccgggatcag ccgaattccg acgtgatgga
     1081 gcacggccat gcatacttct agagaagtcg gccaccgtga aattcgacga cgagatggta
     1141 ctagaatcac cgggacagta cacacctcgt tcatggcctc gagcattcga tgtcgctctc
     1201 acctgcacag cctcgaggta tcgagagggt tctaggatgg accagcgatg tctcgaggaa
     1261 tttagaaaga tttcatgcgc tcgttgtgtg agagccaagg aagcacatta tggggcctat
     1321 aagtaccacg caagggcctg gggttcccat caatcaaatc agtgttcaga cgatttcctc
     1381 aagcgatcat tgcaccgtga tcagtgaagc acagacttca agcttttggc ttgcctactt
     1441 ccacgttccc atccgagatc gacaccgaat tgttgagttc tgtctgatct tcctgcagtc
     1501 gagagcgaag ttccaccatc tagagttatc aacaagtttg tacaaaaaag ctgaacgaga
     1561 aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac
     1621 ataatactgt aaaacacaac atatccagtc actatggcgg cccctcgagg ggccgcatta
     1681 ggcaccccag gctttacact ttatgcttcc ggctcgtata atgtgtggat tttgagttag
     1741 gatccggcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat
     1801 accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt
     1861 gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta
     1921 aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat
     1981 gctcatccgg aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt
     2041 cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa
     2101 taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt
     2161 gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat
     2221 ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc
     2281 cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg
     2341 attcaggttc atcatgccgt ctgtgatggc ttccatgtcg gcagaatgct taatgaatta
     2401 caacagtact gcgatgagtg gcagggcggg gcgtaaacgc gtggatccgg cttactaaaa
     2461 gccagataac agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat
     2521 atgtataccc gaagtatgtc aaaaagaggt gtgctatgaa gcagcgtatt acagtgacag
     2581 ttgacagcga cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt
     2641 aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa
     2701 tcaggaaggg atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga
     2761 gaacagggac tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc
     2821 gtctgtttgt ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc
     2881 ccctggccag tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc
     2941 atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg
     3001 ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta
     3061 acctgatgtt ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc
     3121 gaccatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa
     3181 atctaattta atatattgat atttatatca ttttacgttt ctcgttcagc tttcttgtac
     3241 aaagtggttg ataacagcgc ttagagctcg aatttccccg atcgttcaaa catttggcaa
     3301 taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg
     3361 ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg
     3421 gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag
     3481 cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgggaattg
     3541 gttccggaac caattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     3601 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     3661 gagtgagcta actcacatta ggctgaatta ggcgcgccta tttctgaatt caacacattg
     3721 cggacgtttt taatgtactg aattaacgcc gaattaattc gggggatctg gattttagta
     3781 ctggattttg gttttaggaa ttagaaattt tattgataga agtattttac aaatacaaat
     3841 acatactaag ggtttcttat atgctcaaca catgagcgaa accctatagg aaccctaaat
     3901 tcccttatct gggaactact cacacattat tatggagaaa ctcgaagtta gtatatagtc
     3961 cttccatcac cctctgtgat aatctcctta aaacttgctc ccccaggaat cataggcaga
     4021 acgtgctcct ggtggggaac tatcacatcc agtaaataag gaccgggaga atctagcata
     4081 gtttgaattg ctgctcttag ttcatccttc ttcaccacac gcgcatttgg caccttgaaa
     4141 ccgtctgcaa ctgtacagaa gtcggggtag atctgctcct cgtgatctgg atcacctaag
     4201 taagtatgtg ctctgttggc cttgtagaat ctatcctcca attgtacgac cattcctaga
     4261 tgttggttgt ttaagatcat gatcttcacg ggtaacttct ccacaaaaat tgtagccaac
     4321 tcctgtacgt tcatcacaaa gctaccatcg ccatcgatgt cgatcacagg aatatctggt
     4381 tgagttgcgg cagcccccaa agcagaagga agtccgaatc ccatggctcc aagacctcca
     4441 gatgtcaacc aacgccgcgg ttcgttgtac ttgaaccact gggctgccca catttgatgt
     4501 tggccaacac cagtagatat gatggcattt ccaccagtga gttcgtacag cgtctgtatg
     4561 gcagattgag gagcaatggc atcctcgaat acgggaaact tcattggcca tttctgcttt
     4621 tgttcttcta gttctgccac ccagttggag aagtcgaaag tcctcttttg actcttcaag
     4681 agggcattga tttcacttag cgcaggtttc acatcagcac acatggacac atggggctgt
     4741 ttgttctttc caatttcagc agggtcaata tcgatatgaa cgatgctgga cctgctagca
     4801 aaagtctcca gtttaccagt cactcgatcg tcgaaacgca ctccaaatgc caacaacaga
     4861 tctgcttggt ccacggcgta gttggcgtaa acagttccat gcattccaag cattttcaaa
     4921 cttaatggat cagactcggg atacgctccc aaacccatta aagtcgatgt gataggaatt
     4981 ccggtaagct cgacaaattc cctcaactct tcgctggcgt gattgcaacc tcctccagaa
     5041 tacactacag gcttcttaga actagaaatg agtcttataa tctgctcgat taacgttggt
     5101 tccggtggcc gaggcagtcg ttgtaagtag cccttcagtt gcattggctg agaccaatct
     5161 ggaatagcca tttgctgttg cacgtccttg ggaatatcta ccaagactgg gcccggccta
     5221 cctgaagctg ccaaaaagaa agcctccctg attacgcgag ggatgtcatc aatgttcata
     5281 acaaggtaat tgtgcttggt gatcgatctc gtaacctcca caattggagt ttcttgaaaa
     5341 gcatcggtac caatcatcct actggatact tgacctgtaa tggccactag tggcacactg
     5401 tccaagaggg catcagcgag cccggtaaca agattggtcg ctccaggacc ggatgtggct
     5461 atacaaaccc ctactcgtcc agacgattta gcataacctt cagcagcgaa aatctccccc
     5521 tgctcgtggc gacagagaat gttgcggatg ctgctggaac gtgtaagagc ctggtgaatc
     5581 tccatagacg ctccgccggg ataagcgaac accgttctaa tcccttctcg ctcaagagcc
     5641 tccaccaaaa tgtcggctcc tttgcggggc tcgtcatcct tgtagcgaga ctgaaagtcg
     5701 gacggggttg gtgcaacgag tgacttcccg ctgctaagcc gtggtccact cacatcgact
     5761 gcagactgga tcttctcgct aacagcttgg gctttcactt ccagtgaacg ctcagtcttc
     5821 cgagaggtgt tgtatgcatg tcccctcaga gcggctcccg agaagaaacc actcctcacc
     5881 tttagctctt tagctccctt gcaacaagac cttgccgatg gctgcacctt ctccaggctc
     5941 gctatgctca aaggcacagc tgcgcatcca ctaattgacg ccatgctcga gagagataga
     6001 tttgtagaga gagactggtg atttcagcgt gtcctctcca aatgaaatga acttccttat
     6061 atagaggaag ggtcttgcga aggatagtgg gattgtgcgt catcccttac gtcagtggag
     6121 atatcacatc aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg
     6181 ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg cagaggcatc ttgaacgata
     6241 gcctttcctt tatcgcaatt atggcatttg taggtgccac cttccttttc tactgtcctt
     6301 ttgatgaagt gacagatagc tgggcaatgg aatccgagga ggtttcccga tattaccctt
     6361 tgttgaaaag tctcaatagc cctttggtct tctgagactg tatctttgat attcttggag
     6421 tagacgagag tgtcgtgctc caccatgttc acatcaatcc acttgctttg aagacgtggt
     6481 tggaacgtct tctttttcca cgatgctcct cgtgggtggg ggtccatctt tgggaccact
     6541 gtcggcagag gcatcttgaa cgatagcctt tcctttatcg caatgatggc atttgtaggt
     6601 gccaccttcc ttttctactg tccttttgat gaagtgacag atagctgggc aatggaatcc
     6661 gaggaggttt cccgatatta ccctttgttg aaaagtctca atagcccttt ggtcttctga
     6721 gactgtatct ttgatattct tggagtagac gagagtgtcg tgctccacca tgttggcaag
     6781 ctgctctagc caatacgcaa accgaattca gaaataaatt cagcctaatt cggcgttaat
     6841 tcagtacatt aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca atttgtttac
     6901 accacaatat atcctgccac cagccagcca acagctcccc gaccggcagc tcggcacaaa
     6961 atcaccactc gatacaggca gcccatcagt ccgggacggc gtcagcggga gagccgttgt
     7021 aaggcggcag actttgctca tgttaccgat gctattcgga agaacggcaa ctaagctgcc
     7081 gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt gattgtaacg atgacagagc
     7141 gttgctgcct gtgatcaatt cgggcacgaa cccagtggac ataagcctgt tcggttcgta
     7201 agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca
     7261 gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac
     7321 agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt
     7381 tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca
     7441 tgggggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg
     7501 agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg
     7561 gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa
     7621 caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg
     7681 agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt
     7741 atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta
     7801 tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac
     7861 atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg
     7921 atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg
     7981 gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca
     8041 aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc
     8101 agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct
     8161 cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag
     8221 tcggcaaata atgtctagct agaaattcgt tcaagccgac gccgcttcgc ggcgcggctt
     8281 aactcaagcg ttagatgcac taagcacata attgctcaca gccaaactat caggtcaagt
     8341 ctgcttttat tatttttaag cgtgcataat aagccctaca caaattggga gatatatcat
     8401 gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     8461 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     8521 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     8581 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     8641 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     8701 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     8761 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     8821 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     8881 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     8941 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     9001 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     9061 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     9121 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     9181 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     9241 cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     9301 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc
     9361 gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc
     9421 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     9481 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt
     9541 gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc
     9601 ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg
     9661 cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc
     9721 gctggccaga cagttatgca caggccaggc gggttttaag agttttaata agttttaaag
     9781 agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc
     9841 ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct
     9901 ttgggttccc aatgtacgtg ctatccacag gaaagagacc ttttcgacct ttttcccctg
     9961 ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct
    10021 cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca
    10081 aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct
    10141 tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg
    10201 ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca
    10261 aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt
    10321 acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga
    10381 tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg
    10441 ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca
    10501 ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt
    10561 gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt
    10621 cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg
    10681 ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag
    10741 ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc
    10801 gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg
    10861 gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat
    10921 cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg
    10981 cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta
    11041 ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc
    11101 attgcagggc cggcagacaa cccagccgct tacgcctggc caaccgcccg ttcctccaca
    11161 catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt
    11221 agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga
    11281 tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct
    11341 tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca
    11401 ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt
    11461 ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc
    11521 agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt
    11581 tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat
    11641 gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat
    11701 cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt
    11761 aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat
    11821 cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc
    11881 gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg
    11941 gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact
    12001 gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc
    12061 tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac
    12121 cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc
    12181 gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt
    12241 tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg
    12301 atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg
    12361 atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc
    12421 ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg
    12481 tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc
    12541 tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt
    12601 tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga
    12661 gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg
    12721 tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca
    12781 tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc
    12841 cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt
    12901 ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct
    12961 ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg
    13021 cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc
    13081 ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag
    13141 tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg
    13201 cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg
    13261 aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttgatccc
    13321 gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa cct
//
