LOCUS       sequence-122362-       12659 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12659)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 12659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB233
FEATURES             Location/Qualifiers
     source          1..12659
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1532..1656
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1693..1698,1699..1716,1717..1723)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1777..2436
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1843..1862)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2778..3083
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2962..2981
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3124..3248)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(3259..3261,3262..3264,3265..3975)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3304..3325)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3565..3584)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3912..3933
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      4000..4252
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4286..4302)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4286..4302)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4299..4321)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4310..4326
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4334..4340,4341..4358,4359..4364))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4379..4400
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    4485..4657
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(4714..5247)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MVRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(5310..5986)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(5353..5373)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    6181..6205
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6726..7517
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7763..8351
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8252..8271
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8505..8522
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8537..8677
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8603..8622)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8763..8785
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9021..9215
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9281..10354)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10783..11412)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgggt ctagaccagc cattatagca actatgttaa aagtgacagt tttctctaat
      361 cgggaatctt gtccgtattg aaagttccct gttcagtgac tgagccgctt aataatattt
      421 tcagtttcga gtacgaggga gagattgaac aaattttggg gtttcagggc taaattctgc
      481 aggggcttta gttttgagtc gggtgaaact ttcctaagtc ttggcaatga actcacattc
      541 gccattgtac ctattaaatg acccaaacat ccttgacaac aaattgacaa gtacgggaac
      601 atgatcttca atatgaaatt taatatgtcc tttatgttgg ctttcctcat caatgattgg
      661 aaactcacat acaaagaaaa gcgtgatata ataaatcact agaatcttct ccgctttcta
      721 agacagtaat gatggatcca cgtgagtgtg aaacatcaat atcaacatcg gtaggagtag
      781 agaagtttct aaatcagaaa aagtgaatag acaagacaac ttactccttg aacgagaata
      841 ttcctcgaga aagagggaaa agagtaaaaa gagtaaaaag ccatggtaca gaatagccca
      901 ttccatggtt attgcagtca aaccgctacg ccagcaaaaa cggaacgaaa ctaaacccgt
      961 tccacaaatt gcgaaagatg gaatgtattc gccatgtata tttcgagatt attatgagac
     1021 ttccggtccc gggaagatgg cctgggttca ccgggatcag ccgaattccg acgtgatgga
     1081 gcacggccat gcatacttct agagaagtcg gccaccgtga aattcgacga cgagatggta
     1141 ctagaatcac cgggacagta cacacctcgt tcatggcctc gagcattcga tgtcgctctc
     1201 acctgcacag cctcgaggta tcgagagggt tctaggatgg accagcgatg tctcgaggaa
     1261 tttagaaaga tttcatgcgc tcgttgtgtg agagccaagg aagcacatta tggggcctat
     1321 aagtaccacg caagggcctg gggttcccat caatcaaatc agtgttcaga cgatttcctc
     1381 aagcgatcat tgcaccgtga tcagtgaagc acagacttca agcttttggc ttgcctactt
     1441 ccacgttccc atccgagatc gacaccgaat tgttgagttc tgtctgatct tcctgcagtc
     1501 gagagcgaag ttccaccatc tagagttatc aacaagtttg tacaaaaaag ctgaacgaga
     1561 aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac
     1621 ataatactgt aaaacacaac atatccagtc actatggcgg cccctcgagg ggccgcatta
     1681 ggcaccccag gctttacact ttatgcttcc ggctcgtata atgtgtggat tttgagttag
     1741 gatccgtcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat
     1801 accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt
     1861 gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta
     1921 aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat
     1981 gctcatccgg aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt
     2041 cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa
     2101 taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt
     2161 gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat
     2221 ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc
     2281 cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg
     2341 attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta
     2401 caacagtact gcgatgagtg gcagggcggg gcgtaaagat ctggatccgg cttactaaaa
     2461 gccagataac agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat
     2521 atgtataccc gaagtatgtc aaaaagaggt atgctatgaa gcagcgtatt acagtgacag
     2581 ttgacagcga cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt
     2641 aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa
     2701 tcaggaaggg atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga
     2761 gaacaggggc tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc
     2821 gtctgtttgt ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc
     2881 ccctggccag tgcacgtctg ctgtcagata aagtcccccg tgaactttac ccggtggtgc
     2941 atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg
     3001 ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta
     3061 acctgatgtt ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc
     3121 gaccatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa
     3181 atctaattta atatattgat atttatatca ttttacgttt ctcgttcagc tttcttgtac
     3241 aaagtggttg ataacagcat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     3301 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     3361 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     3421 cccgtgccct ggcccaccct cgtgaccacc ttcggctacg gcctgatgtg cttcgcccgc
     3481 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     3541 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     3601 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     3661 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     3721 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     3781 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     3841 ctgctgcccg acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag
     3901 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     3961 gacgagctgt acaagtgagc ttagagctcg aatttccccg atcgttcaaa catttggcaa
     4021 taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg
     4081 ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg
     4141 gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag
     4201 cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgggaattg
     4261 gttccggaac caattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     4321 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     4381 gagtgagcta actcacatta ggctgaatta ggcgcgccta tttctgaatt caacacattg
     4441 cggacgtttt taatgtactg aattaacgcc gaattaattc gggggatctg gattttagta
     4501 ctggattttg gttttaggaa ttagatttta ttgatagaag tattttacaa atacaaatac
     4561 atactaaggg tttcttatat gctcaacaca tgagcgaaac cctataggaa ccctaattcc
     4621 cttatctggg aactactcac acattattat ggagaaactc gagcttgtcg atcgactcta
     4681 ggccgggaag ccgatctcgg cttgaacgaa ttgttaggtg gcggtacttg ggtcgatatc
     4741 aaagtgcatc acttcttccc gtatgcccaa ctttgtatag agagccactg cgggatcgtc
     4801 accgtaatct gcttgcacgt agatcacata agcaccaagc gcgttggcct catgcttgag
     4861 gagattgatg agcgcggtgg caatgccctg cctccggtgc tcgccggaga ctgcgagatc
     4921 atagatatag atctcactac gcggctgctc aaacttgggc agaacgtaag ccgcgagagc
     4981 gccaacaacc gcttcttggt cgaaggcagc aagcgcgatg aatgtcttac tacggagcaa
     5041 gttcccgagg taatcggagt ccggctgatg ttgggagtag gtggctacgt ctccgaactc
     5101 acgaccgaaa agatcaagag cagcccgcat ggatttgact tggtcagggc cgagcctaca
     5161 tgtgcgaatg atgcccatac ttgagccacc taactttgtt ttagggcgac tgccctgctg
     5221 cgtaacatcg ttgctgctgc gtaccatggt cgatcgacag atctgcgaaa gctcgagaga
     5281 gatagatttg tagagagaga ctggtgattt cagcgtgtcc tctccaaatg aaatgaactt
     5341 ccttatatag aggaagggtc ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca
     5401 gtggagatat cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc
     5461 acgatgctcc tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttga
     5521 acgatagcct ttcctttatc gcaatgatgg catttgtagg tgccaccttc cttttctact
     5581 gtccttttga tgaagtgaca gatagctggg caatggaatc cgaggaggtt tcccgatatt
     5641 accctttgtt gaaaagtctc aatagccctt tggtcttctg agactgtatc tttgatattc
     5701 ttggagtaga cgagagtgtc gtgctccacc atgttcacat caatccactt gctttgaaga
     5761 cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg
     5821 accactgtcg gcagaggcat cttgaacgat agcctttcct ttatcgcaat gatggcattt
     5881 gtaggtgcca ccttcctttt ctactgtcct tttgatgaag tgacagatag ctgggcaatg
     5941 gaatccgagg aggtttcccg atattaccct ttgttgaaaa gtctcaatag ccctttggtc
     6001 ttctgagact gtatctttga tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt
     6061 ggcaagctgc tctagccaat acgcaaaccg aattcagaaa taaattcagc ctaattcggc
     6121 gttaattcag tacattaaaa acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt
     6181 gtttacacca caatatatcc tgccaccagc cagccaacag ctccccgacc ggcagctcgg
     6241 cacaaaatca ccactcgata caggcagccc atcagtccgg gacggcgtca gcgggagagc
     6301 cgttgtaagg cggcagactt tgctcatgtt accgatgcta ttcggaagaa cggcaactaa
     6361 gctgccgggt ttgaaacacg gatgatctcg cggagggtag catgttgatt gtaacgatga
     6421 cagagcgttg ctgcctgtga tcaattcggg cacgaaccca gtggacataa gcctgttcgg
     6481 ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg
     6541 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg
     6601 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
     6661 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa
     6721 acatcatggg ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
     6781 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
     6841 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg
     6901 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
     6961 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
     7021 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
     7081 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     7141 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     7201 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     7261 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     7321 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
     7381 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
     7441 tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca
     7501 aggtagtcgg caaataatgt ctagctagaa attcgttcaa gccgacgccg cttcgcggcg
     7561 cggcttaact caagcgttag atgcactaag cacataattg ctcacagcca aactatcagg
     7621 tcaagtctgc ttttattatt tttaagcgtg cataataagc cctacacaaa ttgggagata
     7681 tatcatgcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     7741 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     7801 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     7861 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     7921 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     7981 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     8041 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     8101 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     8161 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     8221 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     8281 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     8341 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     8401 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     8461 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     8521 aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     8581 accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata
     8641 cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc
     8701 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     8761 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcaggg
     8821 tgccttgatg tgggcgccgg cggtcgagtg gcgacggcgc ggcttgtccg cgccctggta
     8881 gattgcctgg ccgtaggcca gccatttttg agcggccagc ggccgcgata ggccgacgcg
     8941 aagcggcggg gcgtagggag cgcagcgacc gaagggtagg cgctttttgc agctcttcgg
     9001 ctgtgcgctg gccagacagt tatgcacagg ccaggcgggt tttaagagtt ttaataagtt
     9061 ttaaagagtt ttaggcggaa aaatcgcctt ttttctcttt tatatcagtc acttacatgt
     9121 gtgaccggtt cccaatgtac ggctttgggt tcccaatgta cgggttccgg ttcccaatgt
     9181 acggctttgg gttcccaatg tacgtgctat ccacaggaaa gagacctttt cgaccttttt
     9241 cccctgctag ggcaatttgc cctagcatct gctccgtaca ttaggaaccg gcggatgctt
     9301 cgccctcgat caggttgcgg tagcgcatga ctaggatcgg gccagcctgc cccgcctcct
     9361 ccttcaaatc gtactccggc aggtcatttg acccgatcag cttgcgcacg gtgaaacaga
     9421 acttcttgaa ctctccggcg ctgccactgc gttcgtagat cgtcttgaac aaccatctgg
     9481 cttctgcctt gcctgcggcg cggcgtgcca ggcggtagag aaaacggccg atgccgggat
     9541 cgatcaaaaa gtaatcgggg tgaaccgtca gcacgtccgg gttcttgcct tctgtgatct
     9601 cgcggtacat ccaatcagct agctcgatct cgatgtactc cggccgcccg gtttcgctct
     9661 ttacgatctt gtagcggcta atcaaggctt caccctcgga taccgtcacc aggcggccgt
     9721 tcttggcctt cttcgtacgc tgcatggcaa cgtgcgtggt gtttaaccga atgcaggttt
     9781 ctaccaggtc gtctttctgc tttccgccat cggctcgccg gcagaacttg agtacgtccg
     9841 caacgtgtgg acggaacacg cggccgggct tgtctccctt cccttcccgg tatcggttca
     9901 tggattcggt tagatgggaa accgccatca gtaccaggtc gtaatcccac acactggcca
     9961 tgccggccgg ccctgcggaa acctctacgt gcccgtctgg aagctcgtag cggatcacct
    10021 cgccagctcg tcggtcacgc ttcgacagac ggaaaacggc cacgtccatg atgctgcgac
    10081 tatcgcgggt gcccacgtca tagagcatcg gaacgaaaaa atctggttgc tcgtcgccct
    10141 tgggcggctt cctaatcgac ggcgcaccgg ctgccggcgg ttgccgggat tctttgcgga
    10201 ttcgatcagc ggccgcttgc cacgattcac cggggcgtgc ttctgcctcg atgcgttgcc
    10261 gctgggcggc ctgcgcggcc ttcaacttct ccaccaggtc atcacccagc gccgcgccga
    10321 tttgtaccgg gccggatggt ttgcgaccgc tcacgccgat tcctcgggct tgggggttcc
    10381 agtgccattg cagggccggc agacaaccca gccgcttacg cctggccaac cgcccgttcc
    10441 tccacacatg gggcattcca cggcgtcggt gcctggttgt tcttgatttt ccatgccgcc
    10501 tcctttagcc gctaaaattc atctactcat ttattcattt gctcatttac tctggtagct
    10561 gcgcgatgta ttcagatagc agctcggtaa tggtcttgcc ttggcgtacc gcgtacatct
    10621 tcagcttggt gtgatcctcc gccggcaact gaaagttgac ccgcttcatg gctggcgtgt
    10681 ctgccaggct ggccaacgtt gcagccttgc tgctgcgtgc gctcggacgg ccggcactta
    10741 gcgtgtttgt gcttttgctc attttctctt tacctcatta actcaaatga gttttgattt
    10801 aatttcagcg gccagcgcct ggacctcgcg ggcagcgtcg ccctcgggtt ctgattcaag
    10861 aacggttgtg ccggcggcgg cagtgcctgg gtagctcacg cgctgcgtga tacgggactc
    10921 aagaatgggc agctcgtacc cggccagcgc ctcggcaacc tcaccgccga tgcgcgtgcc
    10981 tttgatcgcc cgcgacacga caaaggccgc ttgtagcctt ccatccgtga cctcaatgcg
    11041 ctgcttaacc agctccacca ggtcggcggt ggcccatatg tcgtaagggc ttggctgcac
    11101 cggaatcagc acgaagtcgg ctgccttgat cgcggacaca gccaagtccg ccgcctgggg
    11161 cgctccgtcg atcactacga agtcgcgccg gccgatggcc ttcacgtcgc ggtcaatcgt
    11221 cgggcggtcg atgccgacaa cggttagcgg ttgatcttcc cgcacggccg cccaatcgcg
    11281 ggcactgccc tggggatcgg aatcgactaa cagaacatcg gccccggcga gttgcagggc
    11341 gcgggctaga tgggttgcga tggtcgtctt gcctgacccg cctttctggt taagtacagc
    11401 gataaccttc atgcgttccc cttgcgtatt tgtttattta ctcatcgcat catatacgca
    11461 gcgaccgcat gacgcaagct gttttactca aatacacatc acctttttag acggcggcgc
    11521 tcggtttctt cagcggccaa gctggccggc caggccgcca gcttggcatc agacaaaccg
    11581 gccaggattt catgcagccg cacggttgag acgtgcgcgg gcggctcgaa cacgtacccg
    11641 gccgcgatca tctccgcctc gatctcttcg gtaatgaaaa acggttcgtc ctggccgtcc
    11701 tggtgcggtt tcatgcttgt tcctcttggc gttcattctc ggcggccgcc agggcgtcgg
    11761 cctcggtcaa tgcgtcctca cggaaggcac cgcgccgcct ggcctcggtg ggcgtcactt
    11821 cctcgctgcg ctcaagtgcg cggtacaggg tcgagcgatg cacgccaagc agtgcagccg
    11881 cctctttcac ggtgcggcct tcctggtcga tcagctcgcg ggcgtgcgcg atctgtgccg
    11941 gggtgagggt agggcggggg ccaaacttca cgcctcgggc cttggcggcc tcgcgcccgc
    12001 tccgggtgcg gtcgatgatt agggaacgct cgaactcggc aatgccggcg aacacggtca
    12061 acaccatgcg gccggccggc gtggtggtgt cggcccacgg ctctgccagg ctacgcaggc
    12121 ccgcgccggc ctcctggatg cgctcggcaa tgtccagtag gtcgcgggtg ctgcgggcca
    12181 ggcggtctag cctggtcact gtcacaacgt cgccagggcg taggtggtca agcatcctgg
    12241 ccagctccgg gcggtcgcgc ctggtgccgg tgatcttctc ggaaaacagc ttggtgcagc
    12301 cggccgcgtg cagttcggcc cgttggttgg tcaagtcctg gtcgtcggtg ctgacgcggg
    12361 catagcccag caggccagcg gcggcgctct tgttcatggc gtaatgtctc cggttctagt
    12421 cgcaagtatt ctactttatg cgactaaaac acgcgacaag aaaacgccag gaaaagggca
    12481 gggcggcagc ctgtcgcgta acttaggact tgtgcgacat gtcgttttca gaagacggct
    12541 gcactgaacg tcagaagccg actgcactat agcagcggag gggttggatc aaagtacttt
    12601 gatcccgagg ggaaccctgt ggttggcatg cacatacaaa tggacgaacg gataaacct
//
