LOCUS       sequence-122365-       13290 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13290)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 13290)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB134
FEATURES             Location/Qualifiers
     source          1..13290
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1532..1656
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1693..1698,1699..1716,1717..1723)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1777..2436
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1843..1862)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2778..3083
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2962..2981
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3124..3248)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      4150..4402
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4436..4452)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4436..4452)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4449..4471)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4460..4476
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4484..4490,4491..4508,4509..4514))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4529..4550
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    4635..4809
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(4850..5875)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(5942..6617)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     misc_feature    6812..6836
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7357..8148
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8394..8982
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8883..8902
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9136..9153
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9168..9308
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9234..9253)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9394..9416
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9652..9846
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9912..10985)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11414..12043)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgggt ctagaccagc cattatagca actatgttaa aagtgacagt tttctctaat
      361 cgggaatctt gtccgtattg aaagttccct gttcagtgac tgagccgctt aataatattt
      421 tcagtttcga gtacgaggga gagattgaac aaattttggg gtttcagggc taaattctgc
      481 aggggcttta gttttgagtc gggtgaaact ttcctaagtc ttggcaatga actcacattc
      541 gccattgtac ctattaaatg acccaaacat ccttgacaac aaattgacaa gtacgggaac
      601 atgatcttca atatgaaatt taatatgtcc tttatgttgg ctttcctcat caatgattgg
      661 aaactcacat acaaagaaaa gcgtgatata ataaatcact agaatcttct ccgctttcta
      721 agacagtaat gatggatcca cgtgagtgtg aaacatcaat atcaacatcg gtaggagtag
      781 agaagtttct aaatcagaaa aagtgaatag acaagacaac ttactccttg aacgagaata
      841 ttcctcgaga aagagggaaa agagtaaaaa gagtaaaaag ccatggtaca gaatagccca
      901 ttccatggtt attgcagtca aaccgctacg ccagcaaaaa cggaacgaaa ctaaacccgt
      961 tccacaaatt gcgaaagatg gaatgtattc gccatgtata tttcgagatt attatgagac
     1021 ttccggtccc gggaagatgg cctgggttca ccgggatcag ccgaattccg acgtgatgga
     1081 gcacggccat gcatacttct agagaagtcg gccaccgtga aattcgacga cgagatggta
     1141 ctagaatcac cgggacagta cacacctcgt tcatggcctc gagcattcga tgtcgctctc
     1201 acctgcacag cctcgaggta tcgagagggt tctaggatgg accagcgatg tctcgaggaa
     1261 tttagaaaga tttcatgcgc tcgttgtgtg agagccaagg aagcacatta tggggcctat
     1321 aagtaccacg caagggcctg gggttcccat caatcaaatc agtgttcaga cgatttcctc
     1381 aagcgatcat tgcaccgtga tcagtgaagc acagacttca agcttttggc ttgcctactt
     1441 ccacgttccc atccgagatc gacaccgaat tgttgagttc tgtctgatct tcctgcagtc
     1501 gagagcgaag ttccaccatc tagagttatc aacaagtttg tacaaaaaag ctgaacgaga
     1561 aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac
     1621 ataatactgt aaaacacaac atatccagtc actatggcgg cccctcgagg ggccgcatta
     1681 ggcaccccag gctttacact ttatgcttcc ggctcgtata atgtgtggat tttgagttag
     1741 gatccggcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat
     1801 accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt
     1861 gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta
     1921 aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat
     1981 gctcatccgg aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt
     2041 cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa
     2101 taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt
     2161 gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat
     2221 ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc
     2281 cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg
     2341 attcaggttc atcatgccgt ctgtgatggc ttccatgtcg gcagaatgct taatgaatta
     2401 caacagtact gcgatgagtg gcagggcggg gcgtaaacgc gtggatccgg cttactaaaa
     2461 gccagataac agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat
     2521 atgtataccc gaagtatgtc aaaaagaggt gtgctatgaa gcagcgtatt acagtgacag
     2581 ttgacagcga cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt
     2641 aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa
     2701 tcaggaaggg atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga
     2761 gaacagggac tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc
     2821 gtctgtttgt ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc
     2881 ccctggccag tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc
     2941 atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg
     3001 ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta
     3061 acctgatgtt ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc
     3121 gaccatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa
     3181 atctaattta atatattgat atttatatca ttttacgttt ctcgttcagc tttcttgtac
     3241 aaagtggttg ataacagcat ggctagtgaa gctcgaaaaa caaagaaaaa aatcaaaggg
     3301 attcagcaag ccactgcagg agtctcacaa gacacttcgg aaaatcctaa caaaacaata
     3361 gttcctgcag cattaccaca gctcacccct accttggtgt cactgctgga ggtgattgaa
     3421 cccgaggtgt tgtatgcagg atatgatagc tctgttccag attcagcatg gagaattatg
     3481 accacactca acatgttagg tgggcgtcaa gtgattgcag cagtgaaatg ggcaaaggcg
     3541 ataccaggct tcagaaactt acacctggat gaccaaatga ccctgctaca gtactcatgg
     3601 atgtttctca tggcatttgc cctgggttgg agatcataca gacaatcaag tggaaacctg
     3661 ctctgctttg ctcctgatct gattattaat gagcagagaa tgtctctacc ctgcatgtat
     3721 gaccaatgta aacacatgct gtttgtctcc tctgaattac aaagattgca ggtatcctat
     3781 gaagagtatc tctgtatgaa aaccttactg cttctctcct cagttcctaa ggaaggtctg
     3841 aagagccaag agttatttga tgagattcga atgacttata tcaaagagct aggaaaagcc
     3901 atcgtcaaaa gggaagggaa ctccagtcag aactggcaac ggttttacca actgacaaaa
     3961 cttctggact ccatgcatga ggtggttgag aatctcctta cctactgctt ccagacattt
     4021 ttggataaga ccatgagtat tgaattccca gagatgttag ctgaaatcat cactaatcag
     4081 ataccaaaat attcaaatgg aaatatcaag aagctgctgt ttcatcaaaa atagagctcg
     4141 aatttccccg atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc
     4201 ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac
     4261 atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac
     4321 atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg
     4381 gtgtcatcta tgttactaga tcgggaattg gttccggaac caattcgtaa tcatggtcat
     4441 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     4501 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta ggctgaatta
     4561 ggcgcgccta tttctgaatt caacacattg cggacgtttt taatgtactg aattaacgcc
     4621 gaattaattc gggggatctg gattttagta ctggattttg gttttaggaa ttagaaattt
     4681 tattgataga agtattttac aaatacaaat acatactaag ggtttcttat atgctcaaca
     4741 catgagcgaa accctatagg aaccctaatt cccttatctg ggaactactc acacattatt
     4801 atggagaaac tcgagcttgt cgatcgacag atcccggtcg gcatctactc tatttctttg
     4861 ccctcggacg agtgctgggg cgtcggtttc cactatcggc gagtacttct acacagccat
     4921 cggtccagac ggccgcgctt ctgcgggcga tttgtgtacg cccgacagtc ccggctccgg
     4981 atcggacgat tgcgtcgcat cgaccctgcg cccaagctgc atcatcgaaa ttgccgtcaa
     5041 ccaagctctg atagagttgg tcaagaccaa tgcggagcat atacgcccgg agtcgtggcg
     5101 atcctgcaag ctccggatgc ctccgctcga agtagcgcgt ctgctgctcc atacaagcca
     5161 accacggcct ccagaagaag atgttggcga cctcgtattg ggaatccccg aacatcgcct
     5221 cgctccagtc aatgaccgct gttatgcggc cattgtccgt caggacattg ttggagccga
     5281 aatccgcgtg cacgaggtgc cggacttcgg ggcagtcctc ggcccaaagc atcagctcat
     5341 cgagagcctg cgcgacggac gcactgacgg tgtcgtccat cacagtttgc cagtgataca
     5401 catggggatc agcaatcgcg catatgaaat cacgccatgt agtgtattga ccgattcctt
     5461 gcggtccgaa tgggccgaac ccgctcgtct ggctaagatc ggccgcagcg atcgcatcca
     5521 tagcctccgc gaccggttgt agaacagcgg gcagttcggt ttcaggcagg tcttgcaacg
     5581 tgacaccctg tgaacggcgg gagatgcaat aggtcaggct ctcgctaaac tccccaatgt
     5641 caagcacttc cggaatcggg agcgcggccg atgcaaagtg ccgataaaca taacgatctt
     5701 tgtagaaacc atcggcgcag ctatttaccc gcaggacata tccacgccct cctacatcga
     5761 agctgaaagc acgagattct tcgccctccg agagctgcat caggtcggag acgctgtcga
     5821 acttttcgat cagaaacttc tcgacagacg tcgcggtgag ttcaggcttt ttcatatctc
     5881 attgcccccc ggatctgcga aagctcgaga gagatagatt tgtagagaga gactggtgat
     5941 ttcagcgtgt cctctccaaa tgaaatgaac ttccttatat agaggaaggt cttgcgaagg
     6001 atagtgggat tgtgcgtcat cccttacgtc agtggagata tcacatcaat ccacttgctt
     6061 tgaagacgtg gttggaacgt cttctttttc cacgatgctc ctcgtgggtg ggggtccatc
     6121 tttgggacca ctgtcggcag aggcatcttg aacgatagcc tttcctttat cgcaatgatg
     6181 gcatttgtag gtgccacctt ccttttctac tgtccttttg atgaagtgac agatagctgg
     6241 gcaatggaat ccgaggaggt ttcccgatat taccctttgt tgaaaagtct caatagccct
     6301 ttggtcttct gagactgtat ctttgatatt cttggagtag acgagagtgt cgtgctccac
     6361 catgttcaca tcaatccact tgctttgaag acgtggttgg aacgtcttct ttttccacga
     6421 tgctcctcgt gggtgggggt ccatctttgg gaccactgtc ggcagaggca tcttgaacga
     6481 tagcctttcc tttatcgcaa tgatggcatt tgtaggtgcc accttccttt tctactgtcc
     6541 ttttgatgaa gtgacagata gctgggcaat ggaatccgag gaggtttccc gatattaccc
     6601 tttgttgaaa agtctcaata gccctttggt cttctgagac tgtatctttg atattcttgg
     6661 agtagacgag agtgtcgtgc tccaccatgt tggcaagctg ctctagccaa tacgcaaacc
     6721 gaattcagaa ataaattcag cctaattcgg cgttaattca gtacattaaa aacgtccgca
     6781 atgtgttatt aagttgtcta agcgtcaatt tgtttacacc acaatatatc ctgccaccag
     6841 ccagccaaca gctccccgac cggcagctcg gcacaaaatc accactcgat acaggcagcc
     6901 catcagtccg ggacggcgtc agcgggagag ccgttgtaag gcggcagact ttgctcatgt
     6961 taccgatgct attcggaaga acggcaacta agctgccggg tttgaaacac ggatgatctc
     7021 gcggagggta gcatgttgat tgtaacgatg acagagcgtt gctgcctgtg atcaattcgg
     7081 gcacgaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg
     7141 cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg
     7201 gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag
     7261 cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg
     7321 cagcagggca gtcgccctaa aacaaagtta aacatcatgg gggaagcggt gatcgccgaa
     7381 gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg
     7441 ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt
     7501 gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac
     7561 gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc
     7621 accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa
     7681 tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac
     7741 attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca
     7801 gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa
     7861 accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt
     7921 acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct
     7981 gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga
     8041 caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa
     8101 tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataatg tctagctaga
     8161 aattcgttca agccgacgcc gcttcgcggc gcggcttaac tcaagcgtta gatgcactaa
     8221 gcacataatt gctcacagcc aaactatcag gtcaagtctg cttttattat ttttaagcgt
     8281 gcataataag ccctacacaa attgggagat atatcatgca tgaccaaaat cccttaacgt
     8341 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     8401 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     8461 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     8521 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     8581 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     8641 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     8701 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     8761 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     8821 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     8881 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     8941 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     9001 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     9061 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     9121 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat
     9181 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc
     9241 tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca
     9301 tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     9361 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     9421 caccgtcatc accgaaacgc gcgaggcagg gtgccttgat gtgggcgccg gcggtcgagt
     9481 ggcgacggcg cggcttgtcc gcgccctggt agattgcctg gccgtaggcc agccattttt
     9541 gagcggccag cggccgcgat aggccgacgc gaagcggcgg ggcgtaggga gcgcagcgac
     9601 cgaagggtag gcgctttttg cagctcttcg gctgtgcgct ggccagacag ttatgcacag
     9661 gccaggcggg ttttaagagt tttaataagt tttaaagagt tttaggcgga aaaatcgcct
     9721 tttttctctt ttatatcagt cacttacatg tgtgaccggt tcccaatgta cggctttggg
     9781 ttcccaatgt acgggttccg gttcccaatg tacggctttg ggttcccaat gtacgtgcta
     9841 tccacaggaa agagaccttt tcgacctttt tcccctgcta gggcaatttg ccctagcatc
     9901 tgctccgtac attaggaacc ggcggatgct tcgccctcga tcaggttgcg gtagcgcatg
     9961 actaggatcg ggccagcctg ccccgcctcc tccttcaaat cgtactccgg caggtcattt
    10021 gacccgatca gcttgcgcac ggtgaaacag aacttcttga actctccggc gctgccactg
    10081 cgttcgtaga tcgtcttgaa caaccatctg gcttctgcct tgcctgcggc gcggcgtgcc
    10141 aggcggtaga gaaaacggcc gatgccggga tcgatcaaaa agtaatcggg gtgaaccgtc
    10201 agcacgtccg ggttcttgcc ttctgtgatc tcgcggtaca tccaatcagc tagctcgatc
    10261 tcgatgtact ccggccgccc ggtttcgctc tttacgatct tgtagcggct aatcaaggct
    10321 tcaccctcgg ataccgtcac caggcggccg ttcttggcct tcttcgtacg ctgcatggca
    10381 acgtgcgtgg tgtttaaccg aatgcaggtt tctaccaggt cgtctttctg ctttccgcca
    10441 tcggctcgcc ggcagaactt gagtacgtcc gcaacgtgtg gacggaacac gcggccgggc
    10501 ttgtctccct tcccttcccg gtatcggttc atggattcgg ttagatggga aaccgccatc
    10561 agtaccaggt cgtaatccca cacactggcc atgccggccg gccctgcgga aacctctacg
    10621 tgcccgtctg gaagctcgta gcggatcacc tcgccagctc gtcggtcacg cttcgacaga
    10681 cggaaaacgg ccacgtccat gatgctgcga ctatcgcggg tgcccacgtc atagagcatc
    10741 ggaacgaaaa aatctggttg ctcgtcgccc ttgggcggct tcctaatcga cggcgcaccg
    10801 gctgccggcg gttgccggga ttctttgcgg attcgatcag cggccgcttg ccacgattca
    10861 ccggggcgtg cttctgcctc gatgcgttgc cgctgggcgg cctgcgcggc cttcaacttc
    10921 tccaccaggt catcacccag cgccgcgccg atttgtaccg ggccggatgg tttgcgaccg
    10981 ctcacgccga ttcctcgggc ttgggggttc cagtgccatt gcagggccgg cagacaaccc
    11041 agccgcttac gcctggccaa ccgcccgttc ctccacacat ggggcattcc acggcgtcgg
    11101 tgcctggttg ttcttgattt tccatgccgc ctcctttagc cgctaaaatt catctactca
    11161 tttattcatt tgctcattta ctctggtagc tgcgcgatgt attcagatag cagctcggta
    11221 atggtcttgc cttggcgtac cgcgtacatc ttcagcttgg tgtgatcctc cgccggcaac
    11281 tgaaagttga cccgcttcat ggctggcgtg tctgccaggc tggccaacgt tgcagccttg
    11341 ctgctgcgtg cgctcggacg gccggcactt agcgtgtttg tgcttttgct cattttctct
    11401 ttacctcatt aactcaaatg agttttgatt taatttcagc ggccagcgcc tggacctcgc
    11461 gggcagcgtc gccctcgggt tctgattcaa gaacggttgt gccggcggcg gcagtgcctg
    11521 ggtagctcac gcgctgcgtg atacgggact caagaatggg cagctcgtac ccggccagcg
    11581 cctcggcaac ctcaccgccg atgcgcgtgc ctttgatcgc ccgcgacacg acaaaggccg
    11641 cttgtagcct tccatccgtg acctcaatgc gctgcttaac cagctccacc aggtcggcgg
    11701 tggcccatat gtcgtaaggg cttggctgca ccggaatcag cacgaagtcg gctgccttga
    11761 tcgcggacac agccaagtcc gccgcctggg gcgctccgtc gatcactacg aagtcgcgcc
    11821 ggccgatggc cttcacgtcg cggtcaatcg tcgggcggtc gatgccgaca acggttagcg
    11881 gttgatcttc ccgcacggcc gcccaatcgc gggcactgcc ctggggatcg gaatcgacta
    11941 acagaacatc ggccccggcg agttgcaggg cgcgggctag atgggttgcg atggtcgtct
    12001 tgcctgaccc gcctttctgg ttaagtacag cgataacctt catgcgttcc ccttgcgtat
    12061 ttgtttattt actcatcgca tcatatacgc agcgaccgca tgacgcaagc tgttttactc
    12121 aaatacacat caccttttta gacggcggcg ctcggtttct tcagcggcca agctggccgg
    12181 ccaggccgcc agcttggcat cagacaaacc ggccaggatt tcatgcagcc gcacggttga
    12241 gacgtgcgcg ggcggctcga acacgtaccc ggccgcgatc atctccgcct cgatctcttc
    12301 ggtaatgaaa aacggttcgt cctggccgtc ctggtgcggt ttcatgcttg ttcctcttgg
    12361 cgttcattct cggcggccgc cagggcgtcg gcctcggtca atgcgtcctc acggaaggca
    12421 ccgcgccgcc tggcctcggt gggcgtcact tcctcgctgc gctcaagtgc gcggtacagg
    12481 gtcgagcgat gcacgccaag cagtgcagcc gcctctttca cggtgcggcc ttcctggtcg
    12541 atcagctcgc gggcgtgcgc gatctgtgcc ggggtgaggg tagggcgggg gccaaacttc
    12601 acgcctcggg ccttggcggc ctcgcgcccg ctccgggtgc ggtcgatgat tagggaacgc
    12661 tcgaactcgg caatgccggc gaacacggtc aacaccatgc ggccggccgg cgtggtggtg
    12721 tcggcccacg gctctgccag gctacgcagg cccgcgccgg cctcctggat gcgctcggca
    12781 atgtccagta ggtcgcgggt gctgcgggcc aggcggtcta gcctggtcac tgtcacaacg
    12841 tcgccagggc gtaggtggtc aagcatcctg gccagctccg ggcggtcgcg cctggtgccg
    12901 gtgatcttct cggaaaacag cttggtgcag ccggccgcgt gcagttcggc ccgttggttg
    12961 gtcaagtcct ggtcgtcggt gctgacgcgg gcatagccca gcaggccagc ggcggcgctc
    13021 ttgttcatgg cgtaatgtct ccggttctag tcgcaagtat tctactttat gcgactaaaa
    13081 cacgcgacaa gaaaacgcca ggaaaagggc agggcggcag cctgtcgcgt aacttaggac
    13141 ttgtgcgaca tgtcgttttc agaagacggc tgcactgaac gtcagaagcc gactgcacta
    13201 tagcagcgga ggggttggat caaagtactt tgatcccgag gggaaccctg tggttggcat
    13261 gcacatacaa atggacgaac ggataaacct
//
