LOCUS       sequence-122367-       14243 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14243)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 14243)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB334
FEATURES             Location/Qualifiers
     source          1..14243
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1532..1656
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1693..1698,1699..1716,1717..1723)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1777..2436
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1843..1862)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2778..3083
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2962..2981
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3124..3248)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      4150..4402
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4436..4452)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4436..4452)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4449..4471)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4460..4476
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4484..4490,4491..4508,4509..4514))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4529..4550
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    4635..4810
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(6894..7570)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(6937..6957)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    7765..7789
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             8310..9101
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      9347..9935
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9836..9855
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10089..10106
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    10121..10261
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(10187..10206)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10347..10369
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      10605..10799
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10865..11938)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(12367..12996)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgggt ctagaccagc cattatagca actatgttaa aagtgacagt tttctctaat
      361 cgggaatctt gtccgtattg aaagttccct gttcagtgac tgagccgctt aataatattt
      421 tcagtttcga gtacgaggga gagattgaac aaattttggg gtttcagggc taaattctgc
      481 aggggcttta gttttgagtc gggtgaaact ttcctaagtc ttggcaatga actcacattc
      541 gccattgtac ctattaaatg acccaaacat ccttgacaac aaattgacaa gtacgggaac
      601 atgatcttca atatgaaatt taatatgtcc tttatgttgg ctttcctcat caatgattgg
      661 aaactcacat acaaagaaaa gcgtgatata ataaatcact agaatcttct ccgctttcta
      721 agacagtaat gatggatcca cgtgagtgtg aaacatcaat atcaacatcg gtaggagtag
      781 agaagtttct aaatcagaaa aagtgaatag acaagacaac ttactccttg aacgagaata
      841 ttcctcgaga aagagggaaa agagtaaaaa gagtaaaaag ccatggtaca gaatagccca
      901 ttccatggtt attgcagtca aaccgctacg ccagcaaaaa cggaacgaaa ctaaacccgt
      961 tccacaaatt gcgaaagatg gaatgtattc gccatgtata tttcgagatt attatgagac
     1021 ttccggtccc gggaagatgg cctgggttca ccgggatcag ccgaattccg acgtgatgga
     1081 gcacggccat gcatacttct agagaagtcg gccaccgtga aattcgacga cgagatggta
     1141 ctagaatcac cgggacagta cacacctcgt tcatggcctc gagcattcga tgtcgctctc
     1201 acctgcacag cctcgaggta tcgagagggt tctaggatgg accagcgatg tctcgaggaa
     1261 tttagaaaga tttcatgcgc tcgttgtgtg agagccaagg aagcacatta tggggcctat
     1321 aagtaccacg caagggcctg gggttcccat caatcaaatc agtgttcaga cgatttcctc
     1381 aagcgatcat tgcaccgtga tcagtgaagc acagacttca agcttttggc ttgcctactt
     1441 ccacgttccc atccgagatc gacaccgaat tgttgagttc tgtctgatct tcctgcagtc
     1501 gagagcgaag ttccaccatc tagagttatc aacaagtttg tacaaaaaag ctgaacgaga
     1561 aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac
     1621 ataatactgt aaaacacaac atatccagtc actatggcgg cccctcgagg ggccgcatta
     1681 ggcaccccag gctttacact ttatgcttcc ggctcgtata atgtgtggat tttgagttag
     1741 gatccggcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat
     1801 accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt
     1861 gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta
     1921 aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat
     1981 gctcatccgg aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt
     2041 cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa
     2101 taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt
     2161 gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat
     2221 ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc
     2281 cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg
     2341 attcaggttc atcatgccgt ctgtgatggc ttccatgtcg gcagaatgct taatgaatta
     2401 caacagtact gcgatgagtg gcagggcggg gcgtaaacgc gtggatccgg cttactaaaa
     2461 gccagataac agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat
     2521 atgtataccc gaagtatgtc aaaaagaggt gtgctatgaa gcagcgtatt acagtgacag
     2581 ttgacagcga cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt
     2641 aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa
     2701 tcaggaaggg atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga
     2761 gaacagggac tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc
     2821 gtctgtttgt ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc
     2881 ccctggccag tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc
     2941 atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg
     3001 ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta
     3061 acctgatgtt ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc
     3121 gaccatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa
     3181 atctaattta atatattgat atttatatca ttttacgttt ctcgttcagc tttcttgtac
     3241 aaagtggttg ataacagcat ggctagtgaa gctcgaaaaa caaagaaaaa aatcaaaggg
     3301 attcagcaag ccactgcagg agtctcacaa gacacttcgg aaaatcctaa caaaacaata
     3361 gttcctgcag cattaccaca gctcacccct accttggtgt cactgctgga ggtgattgaa
     3421 cccgaggtgt tgtatgcagg atatgatagc tctgttccag attcagcatg gagaattatg
     3481 accacactca acatgttagg tgggcgtcaa gtgattgcag cagtgaaatg ggcaaaggcg
     3541 ataccaggct tcagaaactt acacctggat gaccaaatga ccctgctaca gtactcatgg
     3601 atgtttctca tggcatttgc cctgggttgg agatcataca gacaatcaag tggaaacctg
     3661 ctctgctttg ctcctgatct gattattaat gagcagagaa tgtctctacc ctgcatgtat
     3721 gaccaatgta aacacatgct gtttgtctcc tctgaattac aaagattgca ggtatcctat
     3781 gaagagtatc tctgtatgaa aaccttactg cttctctcct cagttcctaa ggaaggtctg
     3841 aagagccaag agttatttga tgagattcga atgacttata tcaaagagct aggaaaagcc
     3901 atcgtcaaaa gggaagggaa ctccagtcag aactggcaac ggttttacca actgacaaaa
     3961 cttctggact ccatgcatga ggtggttgag aatctcctta cctactgctt ccagacattt
     4021 ttggataaga ccatgagtat tgaattccca gagatgttag ctgaaatcat cactaatcag
     4081 ataccaaaat attcaaatgg aaatatcaag aagctgctgt ttcatcaaaa atagagctcg
     4141 aatttccccg atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc
     4201 ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac
     4261 atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac
     4321 atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg
     4381 gtgtcatcta tgttactaga tcgggaattg gttccggaac caattcgtaa tcatggtcat
     4441 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     4501 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta ggctgaatta
     4561 ggcgcgccta tttctgaatt caacacattg cggacgtttt taatgtactg aattaacgcc
     4621 gaattaattc gggggatctg gattttagta ctggattttg gttttaggaa ttagaaattt
     4681 tattgataga agtattttac aaatacaaat acatactaag ggtttcttat atgctcaaca
     4741 catgagcgaa accctatagg aaccctaaat tcccttatct gggaactact cacacattat
     4801 tatggagaaa ctcgaagtta gtatatagtc cttccatcac cctctgtgat aatctcctta
     4861 aaacttgctc ccccaggaat cataggcaga acgtgctcct ggtggggaac tatcacatcc
     4921 agtaaataag gaccgggaga atctagcata gtttgaattg ctgctcttag ttcatccttc
     4981 ttcaccacac gcgcatttgg caccttgaaa ccgtctgcaa ctgtacagaa gtcggggtag
     5041 atctgctcct cgtgatctgg atcacctaag taagtatgtg ctctgttggc cttgtagaat
     5101 ctatcctcca attgtacgac cattcctaga tgttggttgt ttaagatcat gatcttcacg
     5161 ggtaacttct ccacaaaaat tgtagccaac tcctgtacgt tcatcacaaa gctaccatcg
     5221 ccatcgatgt cgatcacagg aatatctggt tgagttgcgg cagcccccaa agcagaagga
     5281 agtccgaatc ccatggctcc aagacctcca gatgtcaacc aacgccgcgg ttcgttgtac
     5341 ttgaaccact gggctgccca catttgatgt tggccaacac cagtagatat gatggcattt
     5401 ccaccagtga gttcgtacag cgtctgtatg gcagattgag gagcaatggc atcctcgaat
     5461 acgggaaact tcattggcca tttctgcttt tgttcttcta gttctgccac ccagttggag
     5521 aagtcgaaag tcctcttttg actcttcaag agggcattga tttcacttag cgcaggtttc
     5581 acatcagcac acatggacac atggggctgt ttgttctttc caatttcagc agggtcaata
     5641 tcgatatgaa cgatgctgga cctgctagca aaagtctcca gtttaccagt cactcgatcg
     5701 tcgaaacgca ctccaaatgc caacaacaga tctgcttggt ccacggcgta gttggcgtaa
     5761 acagttccat gcattccaag cattttcaaa cttaatggat cagactcggg atacgctccc
     5821 aaacccatta aagtcgatgt gataggaatt ccggtaagct cgacaaattc cctcaactct
     5881 tcgctggcgt gattgcaacc tcctccagaa tacactacag gcttcttaga actagaaatg
     5941 agtcttataa tctgctcgat taacgttggt tccggtggcc gaggcagtcg ttgtaagtag
     6001 cccttcagtt gcattggctg agaccaatct ggaatagcca tttgctgttg cacgtccttg
     6061 ggaatatcta ccaagactgg gcccggccta cctgaagctg ccaaaaagaa agcctccctg
     6121 attacgcgag ggatgtcatc aatgttcata acaaggtaat tgtgcttggt gatcgatctc
     6181 gtaacctcca caattggagt ttcttgaaaa gcatcggtac caatcatcct actggatact
     6241 tgacctgtaa tggccactag tggcacactg tccaagaggg catcagcgag cccggtaaca
     6301 agattggtcg ctccaggacc ggatgtggct atacaaaccc ctactcgtcc agacgattta
     6361 gcataacctt cagcagcgaa aatctccccc tgctcgtggc gacagagaat gttgcggatg
     6421 ctgctggaac gtgtaagagc ctggtgaatc tccatagacg ctccgccggg ataagcgaac
     6481 accgttctaa tcccttctcg ctcaagagcc tccaccaaaa tgtcggctcc tttgcggggc
     6541 tcgtcatcct tgtagcgaga ctgaaagtcg gacggggttg gtgcaacgag tgacttcccg
     6601 ctgctaagcc gtggtccact cacatcgact gcagactgga tcttctcgct aacagcttgg
     6661 gctttcactt ccagtgaacg ctcagtcttc cgagaggtgt tgtatgcatg tcccctcaga
     6721 gcggctcccg agaagaaacc actcctcacc tttagctctt tagctccctt gcaacaagac
     6781 cttgccgatg gctgcacctt ctccaggctc gctatgctca aaggcacagc tgcgcatcca
     6841 ctaattgacg ccatgctcga gagagataga tttgtagaga gagactggtg atttcagcgt
     6901 gtcctctcca aatgaaatga acttccttat atagaggaag ggtcttgcga aggatagtgg
     6961 gattgtgcgt catcccttac gtcagtggag atatcacatc aatccacttg ctttgaagac
     7021 gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc atctttggga
     7081 ccactgtcgg cagaggcatc ttgaacgata gcctttcctt tatcgcaatt atggcatttg
     7141 taggtgccac cttccttttc tactgtcctt ttgatgaagt gacagatagc tgggcaatgg
     7201 aatccgagga ggtttcccga tattaccctt tgttgaaaag tctcaatagc cctttggtct
     7261 tctgagactg tatctttgat attcttggag tagacgagag tgtcgtgctc caccatgttc
     7321 acatcaatcc acttgctttg aagacgtggt tggaacgtct tctttttcca cgatgctcct
     7381 cgtgggtggg ggtccatctt tgggaccact gtcggcagag gcatcttgaa cgatagcctt
     7441 tcctttatcg caatgatggc atttgtaggt gccaccttcc ttttctactg tccttttgat
     7501 gaagtgacag atagctgggc aatggaatcc gaggaggttt cccgatatta ccctttgttg
     7561 aaaagtctca atagcccttt ggtcttctga gactgtatct ttgatattct tggagtagac
     7621 gagagtgtcg tgctccacca tgttggcaag ctgctctagc caatacgcaa accgaattca
     7681 gaaataaatt cagcctaatt cggcgttaat tcagtacatt aaaaacgtcc gcaatgtgtt
     7741 attaagttgt ctaagcgtca atttgtttac accacaatat atcctgccac cagccagcca
     7801 acagctcccc gaccggcagc tcggcacaaa atcaccactc gatacaggca gcccatcagt
     7861 ccgggacggc gtcagcggga gagccgttgt aaggcggcag actttgctca tgttaccgat
     7921 gctattcgga agaacggcaa ctaagctgcc gggtttgaaa cacggatgat ctcgcggagg
     7981 gtagcatgtt gattgtaacg atgacagagc gttgctgcct gtgatcaatt cgggcacgaa
     8041 cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac
     8101 gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca
     8161 tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag
     8221 cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg
     8281 gcagtcgccc taaaacaaag ttaaacatca tgggggaagc ggtgatcgcc gaagtatcga
     8341 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
     8401 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc
     8461 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt
     8521 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg
     8581 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag
     8641 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc
     8701 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg
     8761 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa
     8821 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt
     8881 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact
     8941 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt
     9001 atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc
     9061 actacgtgaa aggcgagatc accaaggtag tcggcaaata atgtctagct agaaattcgt
     9121 tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg ttagatgcac taagcacata
     9181 attgctcaca gccaaactat caggtcaagt ctgcttttat tatttttaag cgtgcataat
     9241 aagccctaca caaattggga gatatatcat gcatgaccaa aatcccttaa cgtgagtttt
     9301 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     9361 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     9421 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     9481 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     9541 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     9601 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     9661 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     9721 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     9781 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     9841 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     9901 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     9961 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
    10021 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
    10081 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc
    10141 ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg
    10201 atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc
    10261 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
    10321 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
    10381 atcaccgaaa cgcgcgaggc agggtgcctt gatgtgggcg ccggcggtcg agtggcgacg
    10441 gcgcggcttg tccgcgccct ggtagattgc ctggccgtag gccagccatt tttgagcggc
    10501 cagcggccgc gataggccga cgcgaagcgg cggggcgtag ggagcgcagc gaccgaaggg
    10561 taggcgcttt ttgcagctct tcggctgtgc gctggccaga cagttatgca caggccaggc
    10621 gggttttaag agttttaata agttttaaag agttttaggc ggaaaaatcg ccttttttct
    10681 cttttatatc agtcacttac atgtgtgacc ggttcccaat gtacggcttt gggttcccaa
    10741 tgtacgggtt ccggttccca atgtacggct ttgggttccc aatgtacgtg ctatccacag
    10801 gaaagagacc ttttcgacct ttttcccctg ctagggcaat ttgccctagc atctgctccg
    10861 tacattagga accggcggat gcttcgccct cgatcaggtt gcggtagcgc atgactagga
    10921 tcgggccagc ctgccccgcc tcctccttca aatcgtactc cggcaggtca tttgacccga
    10981 tcagcttgcg cacggtgaaa cagaacttct tgaactctcc ggcgctgcca ctgcgttcgt
    11041 agatcgtctt gaacaaccat ctggcttctg ccttgcctgc ggcgcggcgt gccaggcggt
    11101 agagaaaacg gccgatgccg ggatcgatca aaaagtaatc ggggtgaacc gtcagcacgt
    11161 ccgggttctt gccttctgtg atctcgcggt acatccaatc agctagctcg atctcgatgt
    11221 actccggccg cccggtttcg ctctttacga tcttgtagcg gctaatcaag gcttcaccct
    11281 cggataccgt caccaggcgg ccgttcttgg ccttcttcgt acgctgcatg gcaacgtgcg
    11341 tggtgtttaa ccgaatgcag gtttctacca ggtcgtcttt ctgctttccg ccatcggctc
    11401 gccggcagaa cttgagtacg tccgcaacgt gtggacggaa cacgcggccg ggcttgtctc
    11461 ccttcccttc ccggtatcgg ttcatggatt cggttagatg ggaaaccgcc atcagtacca
    11521 ggtcgtaatc ccacacactg gccatgccgg ccggccctgc ggaaacctct acgtgcccgt
    11581 ctggaagctc gtagcggatc acctcgccag ctcgtcggtc acgcttcgac agacggaaaa
    11641 cggccacgtc catgatgctg cgactatcgc gggtgcccac gtcatagagc atcggaacga
    11701 aaaaatctgg ttgctcgtcg cccttgggcg gcttcctaat cgacggcgca ccggctgccg
    11761 gcggttgccg ggattctttg cggattcgat cagcggccgc ttgccacgat tcaccggggc
    11821 gtgcttctgc ctcgatgcgt tgccgctggg cggcctgcgc ggccttcaac ttctccacca
    11881 ggtcatcacc cagcgccgcg ccgatttgta ccgggccgga tggtttgcga ccgctcacgc
    11941 cgattcctcg ggcttggggg ttccagtgcc attgcagggc cggcagacaa cccagccgct
    12001 tacgcctggc caaccgcccg ttcctccaca catggggcat tccacggcgt cggtgcctgg
    12061 ttgttcttga ttttccatgc cgcctccttt agccgctaaa attcatctac tcatttattc
    12121 atttgctcat ttactctggt agctgcgcga tgtattcaga tagcagctcg gtaatggtct
    12181 tgccttggcg taccgcgtac atcttcagct tggtgtgatc ctccgccggc aactgaaagt
    12241 tgacccgctt catggctggc gtgtctgcca ggctggccaa cgttgcagcc ttgctgctgc
    12301 gtgcgctcgg acggccggca cttagcgtgt ttgtgctttt gctcattttc tctttacctc
    12361 attaactcaa atgagttttg atttaatttc agcggccagc gcctggacct cgcgggcagc
    12421 gtcgccctcg ggttctgatt caagaacggt tgtgccggcg gcggcagtgc ctgggtagct
    12481 cacgcgctgc gtgatacggg actcaagaat gggcagctcg tacccggcca gcgcctcggc
    12541 aacctcaccg ccgatgcgcg tgcctttgat cgcccgcgac acgacaaagg ccgcttgtag
    12601 ccttccatcc gtgacctcaa tgcgctgctt aaccagctcc accaggtcgg cggtggccca
    12661 tatgtcgtaa gggcttggct gcaccggaat cagcacgaag tcggctgcct tgatcgcgga
    12721 cacagccaag tccgccgcct ggggcgctcc gtcgatcact acgaagtcgc gccggccgat
    12781 ggccttcacg tcgcggtcaa tcgtcgggcg gtcgatgccg acaacggtta gcggttgatc
    12841 ttcccgcacg gccgcccaat cgcgggcact gccctgggga tcggaatcga ctaacagaac
    12901 atcggccccg gcgagttgca gggcgcgggc tagatgggtt gcgatggtcg tcttgcctga
    12961 cccgcctttc tggttaagta cagcgataac cttcatgcgt tccccttgcg tatttgttta
    13021 tttactcatc gcatcatata cgcagcgacc gcatgacgca agctgtttta ctcaaataca
    13081 catcaccttt ttagacggcg gcgctcggtt tcttcagcgg ccaagctggc cggccaggcc
    13141 gccagcttgg catcagacaa accggccagg atttcatgca gccgcacggt tgagacgtgc
    13201 gcgggcggct cgaacacgta cccggccgcg atcatctccg cctcgatctc ttcggtaatg
    13261 aaaaacggtt cgtcctggcc gtcctggtgc ggtttcatgc ttgttcctct tggcgttcat
    13321 tctcggcggc cgccagggcg tcggcctcgg tcaatgcgtc ctcacggaag gcaccgcgcc
    13381 gcctggcctc ggtgggcgtc acttcctcgc tgcgctcaag tgcgcggtac agggtcgagc
    13441 gatgcacgcc aagcagtgca gccgcctctt tcacggtgcg gccttcctgg tcgatcagct
    13501 cgcgggcgtg cgcgatctgt gccggggtga gggtagggcg ggggccaaac ttcacgcctc
    13561 gggccttggc ggcctcgcgc ccgctccggg tgcggtcgat gattagggaa cgctcgaact
    13621 cggcaatgcc ggcgaacacg gtcaacacca tgcggccggc cggcgtggtg gtgtcggccc
    13681 acggctctgc caggctacgc aggcccgcgc cggcctcctg gatgcgctcg gcaatgtcca
    13741 gtaggtcgcg ggtgctgcgg gccaggcggt ctagcctggt cactgtcaca acgtcgccag
    13801 ggcgtaggtg gtcaagcatc ctggccagct ccgggcggtc gcgcctggtg ccggtgatct
    13861 tctcggaaaa cagcttggtg cagccggccg cgtgcagttc ggcccgttgg ttggtcaagt
    13921 cctggtcgtc ggtgctgacg cgggcatagc ccagcaggcc agcggcggcg ctcttgttca
    13981 tggcgtaatg tctccggttc tagtcgcaag tattctactt tatgcgacta aaacacgcga
    14041 caagaaaacg ccaggaaaag ggcagggcgg cagcctgtcg cgtaacttag gacttgtgcg
    14101 acatgtcgtt ttcagaagac ggctgcactg aacgtcagaa gccgactgca ctatagcagc
    14161 ggaggggttg gatcaaagta ctttgatccc gaggggaacc ctgtggttgg catgcacata
    14221 caaatggacg aacggataaa cct
//
