LOCUS       sequence-122369-       12743 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12743)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 12743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB135
FEATURES             Location/Qualifiers
     source          1..12743
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        794..1139
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1150..1839
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDMALK
                     LVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGH"
     protein_bind    1855..1979
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2016..2021,2022..2039,2040..2046)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2100..2759
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2166..2185)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3101..3406
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3285..3304
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3447..3571)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3603..3855
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3889..3905)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3889..3905)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3902..3924)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3913..3929
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3937..3943,3944..3961,3962..3967))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3982..4003
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    4088..4262
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(4303..5328)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(5395..6070)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     misc_feature    6265..6289
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6810..7601
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7847..8435
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8336..8355
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8589..8606
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8621..8761
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8687..8706)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8847..8869
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9105..9299
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9365..10438)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10867..11496)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttagat tagccttttc aatttcagaa agaatgctaa cccacagatg gttagagagg
      361 cttacgcagc aggtctcatc aagacgatct acccgagcaa taatctccag gaaatcaaat
      421 accttcccaa gaaggttaaa gatgcagtca aaagattcag gactaactgc atcaagaaca
      481 cagagaaaga tatatttctc aagatcagaa gtactattcc agtatggacg attcaaggct
      541 tgcttcacaa accaaggcaa gtaatagaga ttggagtctc taaaaaggta gttcccactg
      601 aatcaaaggc catggagtca aagattcaaa tagaggacct aacagaactc gccgtaaaga
      661 ctggcgaaca gttcatacag agtctcttac gactcaatga caagaagaaa atcttcgtca
      721 acatggtgga gcacgacaca cttgtctact ccaaaaatat caaagataca gtctcagaag
      781 accaaagggc aattgagact tttcaacaaa gggtaatatc cggaaacctc ctcggattcc
      841 attgcccagc tatctgtcac tttattgtga agatagtgga aaaggaaggt ggctcctaca
      901 aatgccatca ttgcgataaa ggaaaggcca tcgttgaaga tgcctctgcc gacagtggtc
      961 ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt
     1021 cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac gcacaatccc
     1081 actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg gagagaacac
     1141 gagcttacaa tgagcgagct gattaaggag aacatgcaca tgaagctgta catggagggc
     1201 accgtgaaca accaccactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc
     1261 acccagacca tgagaatcaa ggtggtcgag ggcggccctc tccccttcgc cttcgacatc
     1321 ctggctacca gcttcatgta cggcagcaga accttcatca accacaccca gggcatcccc
     1381 gacttcttta agcagtcctt ccctgagggc ttcacatggg agagagtcac cacatacgaa
     1441 gacgggggcg tgctgaccgc tacccaggac accagcctcc aggacggctg cctcatctac
     1501 aacgtcaaga tcagaggggt gaacttccca tccaacggcc ctgtgatgca gaagaaaaca
     1561 ctcggctggg aggccaacac cgagatgctg taccccgctg acggcggcct ggaaggcaga
     1621 agcgacatgg ccctgaagct cgtgggcggg ggccacctga tctgcaactt caagaccaca
     1681 tacagatcca agaaacccgc taagaacctc aagatgcccg gcgtctacta tgtggaccac
     1741 agactggaaa gaatcaagga ggccgacaaa gagacctacg tcgagcagca cgaggtggct
     1801 gtggccagat actgcgacct ccctagcaaa ctggggcaca agctagagtt atcaacaagt
     1861 ttgtacaaaa aagctgaacg agaaacgtaa aatgatataa atatcaatat attaaattag
     1921 attttgcata aaaaacagac tacataatac tgtaaaacac aacatatcca gtcactatgg
     1981 cggcccctcg aggggccgca ttaggcaccc caggctttac actttatgct tccggctcgt
     2041 ataatgtgtg gattttgagt taggatccgt cgagattttc aggagctaag gaagctaaaa
     2101 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac
     2161 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata
     2221 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc
     2281 acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg
     2341 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa
     2401 cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt
     2461 cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga
     2521 atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg
     2581 ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg
     2641 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg
     2701 tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaaa
     2761 gatctggatc cggcttacta aaagccagat aacagtatgc gtatttgcgc gctgattttt
     2821 gcggtataag aatatatact gatatgtata cccgaagtat gtcaaaaaga ggtatgctat
     2881 gaagcagcgt attacagtga cagttgacag cgacagctat cagttgctca aggcatatat
     2941 gatgtcaata tctccggtct ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt
     3001 gccgaacgct ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg gtttattgaa
     3061 atgaacggct cttttgctga cgagaacagg ggctggtgaa atgcagttta aggtttacac
     3121 ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac
     3181 gcccgggcga cggatggtga tccccctggc cagtgcacgt ctgctgtcag ataaagtccc
     3241 ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga
     3301 tatggccagt gtgccggtct ccgttatcgg ggaagaagtg gctgatctca gccaccgcga
     3361 aaatgacatc aaaaacgcca ttaacctgat gttctgggga atataaatgt caggctccct
     3421 tatacacagc cagtctgcag gtcgaccata gtgactggat atgttgtgtt ttacagtatt
     3481 atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg
     3541 tttctcgttc agctttcttg tacaaagtgg ttgataacag cgcttagagc tcgaatttcc
     3601 ccgatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg
     3661 cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat
     3721 gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat
     3781 acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat
     3841 ctatgttact agatcgggaa ttggttccgg aaccaattcg taatcatggt catagctgtt
     3901 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     3961 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaggctgaa ttaggcgcgc
     4021 ctatttctga attcaacaca ttgcggacgt ttttaatgta ctgaattaac gccgaattaa
     4081 ttcgggggat ctggatttta gtactggatt ttggttttag gaattagaaa ttttattgat
     4141 agaagtattt tacaaataca aatacatact aagggtttct tatatgctca acacatgagc
     4201 gaaaccctat aggaacccta attcccttat ctgggaacta ctcacacatt attatggaga
     4261 aactcgagct tgtcgatcga cagatcccgg tcggcatcta ctctatttct ttgccctcgg
     4321 acgagtgctg gggcgtcggt ttccactatc ggcgagtact tctacacagc catcggtcca
     4381 gacggccgcg cttctgcggg cgatttgtgt acgcccgaca gtcccggctc cggatcggac
     4441 gattgcgtcg catcgaccct gcgcccaagc tgcatcatcg aaattgccgt caaccaagct
     4501 ctgatagagt tggtcaagac caatgcggag catatacgcc cggagtcgtg gcgatcctgc
     4561 aagctccgga tgcctccgct cgaagtagcg cgtctgctgc tccatacaag ccaaccacgg
     4621 cctccagaag aagatgttgg cgacctcgta ttgggaatcc ccgaacatcg cctcgctcca
     4681 gtcaatgacc gctgttatgc ggccattgtc cgtcaggaca ttgttggagc cgaaatccgc
     4741 gtgcacgagg tgccggactt cggggcagtc ctcggcccaa agcatcagct catcgagagc
     4801 ctgcgcgacg gacgcactga cggtgtcgtc catcacagtt tgccagtgat acacatgggg
     4861 atcagcaatc gcgcatatga aatcacgcca tgtagtgtat tgaccgattc cttgcggtcc
     4921 gaatgggccg aacccgctcg tctggctaag atcggccgca gcgatcgcat ccatagcctc
     4981 cgcgaccggt tgtagaacag cgggcagttc ggtttcaggc aggtcttgca acgtgacacc
     5041 ctgtgaacgg cgggagatgc aataggtcag gctctcgcta aactccccaa tgtcaagcac
     5101 ttccggaatc gggagcgcgg ccgatgcaaa gtgccgataa acataacgat ctttgtagaa
     5161 accatcggcg cagctattta cccgcaggac atatccacgc cctcctacat cgaagctgaa
     5221 agcacgagat tcttcgccct ccgagagctg catcaggtcg gagacgctgt cgaacttttc
     5281 gatcagaaac ttctcgacag acgtcgcggt gagttcaggc tttttcatat ctcattgccc
     5341 cccggatctg cgaaagctcg agagagatag atttgtagag agagactggt gatttcagcg
     5401 tgtcctctcc aaatgaaatg aacttcctta tatagaggaa ggtcttgcga aggatagtgg
     5461 gattgtgcgt catcccttac gtcagtggag atatcacatc aatccacttg ctttgaagac
     5521 gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc atctttggga
     5581 ccactgtcgg cagaggcatc ttgaacgata gcctttcctt tatcgcaatg atggcatttg
     5641 taggtgccac cttccttttc tactgtcctt ttgatgaagt gacagatagc tgggcaatgg
     5701 aatccgagga ggtttcccga tattaccctt tgttgaaaag tctcaatagc cctttggtct
     5761 tctgagactg tatctttgat attcttggag tagacgagag tgtcgtgctc caccatgttc
     5821 acatcaatcc acttgctttg aagacgtggt tggaacgtct tctttttcca cgatgctcct
     5881 cgtgggtggg ggtccatctt tgggaccact gtcggcagag gcatcttgaa cgatagcctt
     5941 tcctttatcg caatgatggc atttgtaggt gccaccttcc ttttctactg tccttttgat
     6001 gaagtgacag atagctgggc aatggaatcc gaggaggttt cccgatatta ccctttgttg
     6061 aaaagtctca atagcccttt ggtcttctga gactgtatct ttgatattct tggagtagac
     6121 gagagtgtcg tgctccacca tgttggcaag ctgctctagc caatacgcaa accgaattca
     6181 gaaataaatt cagcctaatt cggcgttaat tcagtacatt aaaaacgtcc gcaatgtgtt
     6241 attaagttgt ctaagcgtca atttgtttac accacaatat atcctgccac cagccagcca
     6301 acagctcccc gaccggcagc tcggcacaaa atcaccactc gatacaggca gcccatcagt
     6361 ccgggacggc gtcagcggga gagccgttgt aaggcggcag actttgctca tgttaccgat
     6421 gctattcgga agaacggcaa ctaagctgcc gggtttgaaa cacggatgat ctcgcggagg
     6481 gtagcatgtt gattgtaacg atgacagagc gttgctgcct gtgatcaatt cgggcacgaa
     6541 cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac
     6601 gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca
     6661 tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag
     6721 cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg
     6781 gcagtcgccc taaaacaaag ttaaacatca tgggggaagc ggtgatcgcc gaagtatcga
     6841 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
     6901 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc
     6961 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt
     7021 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg
     7081 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag
     7141 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc
     7201 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg
     7261 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa
     7321 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt
     7381 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact
     7441 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt
     7501 atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc
     7561 actacgtgaa aggcgagatc accaaggtag tcggcaaata atgtctagct agaaattcgt
     7621 tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg ttagatgcac taagcacata
     7681 attgctcaca gccaaactat caggtcaagt ctgcttttat tatttttaag cgtgcataat
     7741 aagccctaca caaattggga gatatatcat gcatgaccaa aatcccttaa cgtgagtttt
     7801 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     7861 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     7921 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     7981 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     8041 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     8101 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     8161 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     8221 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     8281 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     8341 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     8401 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     8461 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     8521 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     8581 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc
     8641 ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg
     8701 atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc
     8761 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     8821 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     8881 atcaccgaaa cgcgcgaggc agggtgcctt gatgtgggcg ccggcggtcg agtggcgacg
     8941 gcgcggcttg tccgcgccct ggtagattgc ctggccgtag gccagccatt tttgagcggc
     9001 cagcggccgc gataggccga cgcgaagcgg cggggcgtag ggagcgcagc gaccgaaggg
     9061 taggcgcttt ttgcagctct tcggctgtgc gctggccaga cagttatgca caggccaggc
     9121 gggttttaag agttttaata agttttaaag agttttaggc ggaaaaatcg ccttttttct
     9181 cttttatatc agtcacttac atgtgtgacc ggttcccaat gtacggcttt gggttcccaa
     9241 tgtacgggtt ccggttccca atgtacggct ttgggttccc aatgtacgtg ctatccacag
     9301 gaaagagacc ttttcgacct ttttcccctg ctagggcaat ttgccctagc atctgctccg
     9361 tacattagga accggcggat gcttcgccct cgatcaggtt gcggtagcgc atgactagga
     9421 tcgggccagc ctgccccgcc tcctccttca aatcgtactc cggcaggtca tttgacccga
     9481 tcagcttgcg cacggtgaaa cagaacttct tgaactctcc ggcgctgcca ctgcgttcgt
     9541 agatcgtctt gaacaaccat ctggcttctg ccttgcctgc ggcgcggcgt gccaggcggt
     9601 agagaaaacg gccgatgccg ggatcgatca aaaagtaatc ggggtgaacc gtcagcacgt
     9661 ccgggttctt gccttctgtg atctcgcggt acatccaatc agctagctcg atctcgatgt
     9721 actccggccg cccggtttcg ctctttacga tcttgtagcg gctaatcaag gcttcaccct
     9781 cggataccgt caccaggcgg ccgttcttgg ccttcttcgt acgctgcatg gcaacgtgcg
     9841 tggtgtttaa ccgaatgcag gtttctacca ggtcgtcttt ctgctttccg ccatcggctc
     9901 gccggcagaa cttgagtacg tccgcaacgt gtggacggaa cacgcggccg ggcttgtctc
     9961 ccttcccttc ccggtatcgg ttcatggatt cggttagatg ggaaaccgcc atcagtacca
    10021 ggtcgtaatc ccacacactg gccatgccgg ccggccctgc ggaaacctct acgtgcccgt
    10081 ctggaagctc gtagcggatc acctcgccag ctcgtcggtc acgcttcgac agacggaaaa
    10141 cggccacgtc catgatgctg cgactatcgc gggtgcccac gtcatagagc atcggaacga
    10201 aaaaatctgg ttgctcgtcg cccttgggcg gcttcctaat cgacggcgca ccggctgccg
    10261 gcggttgccg ggattctttg cggattcgat cagcggccgc ttgccacgat tcaccggggc
    10321 gtgcttctgc ctcgatgcgt tgccgctggg cggcctgcgc ggccttcaac ttctccacca
    10381 ggtcatcacc cagcgccgcg ccgatttgta ccgggccgga tggtttgcga ccgctcacgc
    10441 cgattcctcg ggcttggggg ttccagtgcc attgcagggc cggcagacaa cccagccgct
    10501 tacgcctggc caaccgcccg ttcctccaca catggggcat tccacggcgt cggtgcctgg
    10561 ttgttcttga ttttccatgc cgcctccttt agccgctaaa attcatctac tcatttattc
    10621 atttgctcat ttactctggt agctgcgcga tgtattcaga tagcagctcg gtaatggtct
    10681 tgccttggcg taccgcgtac atcttcagct tggtgtgatc ctccgccggc aactgaaagt
    10741 tgacccgctt catggctggc gtgtctgcca ggctggccaa cgttgcagcc ttgctgctgc
    10801 gtgcgctcgg acggccggca cttagcgtgt ttgtgctttt gctcattttc tctttacctc
    10861 attaactcaa atgagttttg atttaatttc agcggccagc gcctggacct cgcgggcagc
    10921 gtcgccctcg ggttctgatt caagaacggt tgtgccggcg gcggcagtgc ctgggtagct
    10981 cacgcgctgc gtgatacggg actcaagaat gggcagctcg tacccggcca gcgcctcggc
    11041 aacctcaccg ccgatgcgcg tgcctttgat cgcccgcgac acgacaaagg ccgcttgtag
    11101 ccttccatcc gtgacctcaa tgcgctgctt aaccagctcc accaggtcgg cggtggccca
    11161 tatgtcgtaa gggcttggct gcaccggaat cagcacgaag tcggctgcct tgatcgcgga
    11221 cacagccaag tccgccgcct ggggcgctcc gtcgatcact acgaagtcgc gccggccgat
    11281 ggccttcacg tcgcggtcaa tcgtcgggcg gtcgatgccg acaacggtta gcggttgatc
    11341 ttcccgcacg gccgcccaat cgcgggcact gccctgggga tcggaatcga ctaacagaac
    11401 atcggccccg gcgagttgca gggcgcgggc tagatgggtt gcgatggtcg tcttgcctga
    11461 cccgcctttc tggttaagta cagcgataac cttcatgcgt tccccttgcg tatttgttta
    11521 tttactcatc gcatcatata cgcagcgacc gcatgacgca agctgtttta ctcaaataca
    11581 catcaccttt ttagacggcg gcgctcggtt tcttcagcgg ccaagctggc cggccaggcc
    11641 gccagcttgg catcagacaa accggccagg atttcatgca gccgcacggt tgagacgtgc
    11701 gcgggcggct cgaacacgta cccggccgcg atcatctccg cctcgatctc ttcggtaatg
    11761 aaaaacggtt cgtcctggcc gtcctggtgc ggtttcatgc ttgttcctct tggcgttcat
    11821 tctcggcggc cgccagggcg tcggcctcgg tcaatgcgtc ctcacggaag gcaccgcgcc
    11881 gcctggcctc ggtgggcgtc acttcctcgc tgcgctcaag tgcgcggtac agggtcgagc
    11941 gatgcacgcc aagcagtgca gccgcctctt tcacggtgcg gccttcctgg tcgatcagct
    12001 cgcgggcgtg cgcgatctgt gccggggtga gggtagggcg ggggccaaac ttcacgcctc
    12061 gggccttggc ggcctcgcgc ccgctccggg tgcggtcgat gattagggaa cgctcgaact
    12121 cggcaatgcc ggcgaacacg gtcaacacca tgcggccggc cggcgtggtg gtgtcggccc
    12181 acggctctgc caggctacgc aggcccgcgc cggcctcctg gatgcgctcg gcaatgtcca
    12241 gtaggtcgcg ggtgctgcgg gccaggcggt ctagcctggt cactgtcaca acgtcgccag
    12301 ggcgtaggtg gtcaagcatc ctggccagct ccgggcggtc gcgcctggtg ccggtgatct
    12361 tctcggaaaa cagcttggtg cagccggccg cgtgcagttc ggcccgttgg ttggtcaagt
    12421 cctggtcgtc ggtgctgacg cgggcatagc ccagcaggcc agcggcggcg ctcttgttca
    12481 tggcgtaatg tctccggttc tagtcgcaag tattctactt tatgcgacta aaacacgcga
    12541 caagaaaacg ccaggaaaag ggcagggcgg cagcctgtcg cgtaacttag gacttgtgcg
    12601 acatgtcgtt ttcagaagac ggctgcactg aacgtcagaa gccgactgca ctatagcagc
    12661 ggaggggttg gatcaaagta ctttgatccc gaggggaacc ctgtggttgg catgcacata
    12721 caaatggacg aacggataaa cct
//
