LOCUS       sequence-122372-       12528 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12528)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 12528)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB435
FEATURES             Location/Qualifiers
     source          1..12528
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        794..1139
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1150..1839
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDMALK
                     LVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGH"
     protein_bind    1855..1979
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2016..2021,2022..2039,2040..2046)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2100..2759
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2166..2185)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3101..3406
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3285..3304
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3447..3571)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3603..3855
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3889..3905)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3889..3905)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3902..3924)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3913..3929
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3937..3943,3944..3961,3962..3967))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3982..4003
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    4088..4262
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(4319..5116)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4430..4449)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5040..5059
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(5179..5855)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     misc_feature    6050..6074
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6595..7386
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7632..8220
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8121..8140
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8374..8391
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8406..8546
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8472..8491)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8632..8654
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8890..9084
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9150..10223)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10652..11281)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttagat tagccttttc aatttcagaa agaatgctaa cccacagatg gttagagagg
      361 cttacgcagc aggtctcatc aagacgatct acccgagcaa taatctccag gaaatcaaat
      421 accttcccaa gaaggttaaa gatgcagtca aaagattcag gactaactgc atcaagaaca
      481 cagagaaaga tatatttctc aagatcagaa gtactattcc agtatggacg attcaaggct
      541 tgcttcacaa accaaggcaa gtaatagaga ttggagtctc taaaaaggta gttcccactg
      601 aatcaaaggc catggagtca aagattcaaa tagaggacct aacagaactc gccgtaaaga
      661 ctggcgaaca gttcatacag agtctcttac gactcaatga caagaagaaa atcttcgtca
      721 acatggtgga gcacgacaca cttgtctact ccaaaaatat caaagataca gtctcagaag
      781 accaaagggc aattgagact tttcaacaaa gggtaatatc cggaaacctc ctcggattcc
      841 attgcccagc tatctgtcac tttattgtga agatagtgga aaaggaaggt ggctcctaca
      901 aatgccatca ttgcgataaa ggaaaggcca tcgttgaaga tgcctctgcc gacagtggtc
      961 ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt
     1021 cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac gcacaatccc
     1081 actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg gagagaacac
     1141 gagcttacaa tgagcgagct gattaaggag aacatgcaca tgaagctgta catggagggc
     1201 accgtgaaca accaccactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc
     1261 acccagacca tgagaatcaa ggtggtcgag ggcggccctc tccccttcgc cttcgacatc
     1321 ctggctacca gcttcatgta cggcagcaga accttcatca accacaccca gggcatcccc
     1381 gacttcttta agcagtcctt ccctgagggc ttcacatggg agagagtcac cacatacgaa
     1441 gacgggggcg tgctgaccgc tacccaggac accagcctcc aggacggctg cctcatctac
     1501 aacgtcaaga tcagaggggt gaacttccca tccaacggcc ctgtgatgca gaagaaaaca
     1561 ctcggctggg aggccaacac cgagatgctg taccccgctg acggcggcct ggaaggcaga
     1621 agcgacatgg ccctgaagct cgtgggcggg ggccacctga tctgcaactt caagaccaca
     1681 tacagatcca agaaacccgc taagaacctc aagatgcccg gcgtctacta tgtggaccac
     1741 agactggaaa gaatcaagga ggccgacaaa gagacctacg tcgagcagca cgaggtggct
     1801 gtggccagat actgcgacct ccctagcaaa ctggggcaca agctagagtt atcaacaagt
     1861 ttgtacaaaa aagctgaacg agaaacgtaa aatgatataa atatcaatat attaaattag
     1921 attttgcata aaaaacagac tacataatac tgtaaaacac aacatatcca gtcactatgg
     1981 cggcccctcg aggggccgca ttaggcaccc caggctttac actttatgct tccggctcgt
     2041 ataatgtgtg gattttgagt taggatccgt cgagattttc aggagctaag gaagctaaaa
     2101 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac
     2161 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata
     2221 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc
     2281 acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg
     2341 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa
     2401 cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt
     2461 cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga
     2521 atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg
     2581 ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg
     2641 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg
     2701 tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaaa
     2761 gatctggatc cggcttacta aaagccagat aacagtatgc gtatttgcgc gctgattttt
     2821 gcggtataag aatatatact gatatgtata cccgaagtat gtcaaaaaga ggtatgctat
     2881 gaagcagcgt attacagtga cagttgacag cgacagctat cagttgctca aggcatatat
     2941 gatgtcaata tctccggtct ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt
     3001 gccgaacgct ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg gtttattgaa
     3061 atgaacggct cttttgctga cgagaacagg ggctggtgaa atgcagttta aggtttacac
     3121 ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac
     3181 gcccgggcga cggatggtga tccccctggc cagtgcacgt ctgctgtcag ataaagtccc
     3241 ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga
     3301 tatggccagt gtgccggtct ccgttatcgg ggaagaagtg gctgatctca gccaccgcga
     3361 aaatgacatc aaaaacgcca ttaacctgat gttctgggga atataaatgt caggctccct
     3421 tatacacagc cagtctgcag gtcgaccata gtgactggat atgttgtgtt ttacagtatt
     3481 atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg
     3541 tttctcgttc agctttcttg tacaaagtgg ttgataacag cgcttagagc tcgaatttcc
     3601 ccgatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg
     3661 cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat
     3721 gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat
     3781 acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat
     3841 ctatgttact agatcgggaa ttggttccgg aaccaattcg taatcatggt catagctgtt
     3901 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     3961 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaggctgaa ttaggcgcgc
     4021 ctatttctga attcaacaca ttgcggacgt ttttaatgta ctgaattaac gccgaattaa
     4081 ttcgggggat ctggatttta gtactggatt ttggttttag gaattagaaa ttttattgat
     4141 agaagtattt tacaaataca aatacatact aagggtttct tatatgctca acacatgagc
     4201 gaaaccctat aggaacccta attcccttat ctgggaacta ctcacacatt attatggaga
     4261 aactcgagct tgtcgatcga ctctagctag aggatcgatc cgaaccccag agtcccgctc
     4321 agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga gcggcgatac
     4381 cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca atatcacggg
     4441 tagccaacgc tatgtcctga tagcggtccg ccacacccag ccggccacag tcgatgaatc
     4501 cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca tgtgtcacga
     4561 cgagatcctc gccgtcgggc atgcgcgcct tgagcctggc gaacagttcg gctggcgcga
     4621 gcccctgatg ctcttcgtcc agatcatcct gatcgacaag accggcttcc atccgagtac
     4681 gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg
     4741 tatgcagccg ccgcattgca tcagccatga tggatacttt ctcggcagga gcaaggtgag
     4801 atgacaggag atcctgcccc ggcacttcgc ccaatagcag ccagtccctt cccgcttcag
     4861 tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg
     4921 ctgcctcgtc ctggagttca ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg
     4981 ggcgcccctg cgctgacagc cggaacacgg cggcatcaga gcagccgatt gtctgttgtg
     5041 cccagtcata gccgaatagc ctctccaccc aagcggccgg agaacctgcg tgcaatccat
     5101 cttgttcaat ccccatggtc gatcgacaga tctgcgaaag ctcgagagag atagatttgt
     5161 agagagagac tggtgatttc agcgtgtcct ctccaaatga aatgaacttc cttatataga
     5221 ggaagggtct tgcgaaggat agtgggattg tgcgtcatcc cttacgtcag tggagatatc
     5281 acatcaatcc acttgctttg aagacgtggt tggaacgtct tctttttcca cgatgctcct
     5341 cgtgggtggg ggtccatctt tgggaccact gtcggcagag gcatcttgaa cgatagcctt
     5401 tcctttatcg caatgatggc atttgtaggt gccaccttcc ttttctactg tccttttgat
     5461 gaagtgacag atagctgggc aatggaatcc gaggaggttt cccgatatta ccctttgttg
     5521 aaaagtctca atagcccttt ggtcttctga gactgtatct ttgatattct tggagtagac
     5581 gagagtgtcg tgctccacca tgttcacatc aatccacttg ctttgaagac gtggttggaa
     5641 cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg
     5701 cagaggcatc ttgaacgata gcctttcctt tatcgcaatg atggcatttg taggtgccac
     5761 cttccttttc tactgtcctt ttgatgaagt gacagatagc tgggcaatgg aatccgagga
     5821 ggtttcccga tattaccctt tgttgaaaag tctcaatagc cctttggtct tctgagactg
     5881 tatctttgat attcttggag tagacgagag tgtcgtgctc caccatgttg gcaagctgct
     5941 ctagccaata cgcaaaccga attcagaaat aaattcagcc taattcggcg ttaattcagt
     6001 acattaaaaa cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg tttacaccac
     6061 aatatatcct gccaccagcc agccaacagc tccccgaccg gcagctcggc acaaaatcac
     6121 cactcgatac aggcagccca tcagtccggg acggcgtcag cgggagagcc gttgtaaggc
     6181 ggcagacttt gctcatgtta ccgatgctat tcggaagaac ggcaactaag ctgccgggtt
     6241 tgaaacacgg atgatctcgc ggagggtagc atgttgattg taacgatgac agagcgttgc
     6301 tgcctgtgat caattcgggc acgaacccag tggacataag cctgttcggt tcgtaagctg
     6361 taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt
     6421 ggtaacggcg cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct
     6481 atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg
     6541 agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa catcatgggg
     6601 gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc
     6661 catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg
     6721 aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg
     6781 cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt
     6841 ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca
     6901 gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc
     6961 gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc
     7021 gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta
     7081 tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat
     7141 gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc
     7201 gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc
     7261 gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc
     7321 gcagatcagt tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc
     7381 aaataatgtc tagctagaaa ttcgttcaag ccgacgccgc ttcgcggcgc ggcttaactc
     7441 aagcgttaga tgcactaagc acataattgc tcacagccaa actatcaggt caagtctgct
     7501 tttattattt ttaagcgtgc ataataagcc ctacacaaat tgggagatat atcatgcatg
     7561 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     7621 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     7681 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     7741 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     7801 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     7861 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     7921 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     7981 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     8041 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     8101 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     8161 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     8221 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     8281 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     8341 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     8401 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg
     8461 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat
     8521 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
     8581 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     8641 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagggt gccttgatgt
     8701 gggcgccggc ggtcgagtgg cgacggcgcg gcttgtccgc gccctggtag attgcctggc
     8761 cgtaggccag ccatttttga gcggccagcg gccgcgatag gccgacgcga agcggcgggg
     8821 cgtagggagc gcagcgaccg aagggtaggc gctttttgca gctcttcggc tgtgcgctgg
     8881 ccagacagtt atgcacaggc caggcgggtt ttaagagttt taataagttt taaagagttt
     8941 taggcggaaa aatcgccttt tttctctttt atatcagtca cttacatgtg tgaccggttc
     9001 ccaatgtacg gctttgggtt cccaatgtac gggttccggt tcccaatgta cggctttggg
     9061 ttcccaatgt acgtgctatc cacaggaaag agaccttttc gacctttttc ccctgctagg
     9121 gcaatttgcc ctagcatctg ctccgtacat taggaaccgg cggatgcttc gccctcgatc
     9181 aggttgcggt agcgcatgac taggatcggg ccagcctgcc ccgcctcctc cttcaaatcg
     9241 tactccggca ggtcatttga cccgatcagc ttgcgcacgg tgaaacagaa cttcttgaac
     9301 tctccggcgc tgccactgcg ttcgtagatc gtcttgaaca accatctggc ttctgccttg
     9361 cctgcggcgc ggcgtgccag gcggtagaga aaacggccga tgccgggatc gatcaaaaag
     9421 taatcggggt gaaccgtcag cacgtccggg ttcttgcctt ctgtgatctc gcggtacatc
     9481 caatcagcta gctcgatctc gatgtactcc ggccgcccgg tttcgctctt tacgatcttg
     9541 tagcggctaa tcaaggcttc accctcggat accgtcacca ggcggccgtt cttggccttc
     9601 ttcgtacgct gcatggcaac gtgcgtggtg tttaaccgaa tgcaggtttc taccaggtcg
     9661 tctttctgct ttccgccatc ggctcgccgg cagaacttga gtacgtccgc aacgtgtgga
     9721 cggaacacgc ggccgggctt gtctcccttc ccttcccggt atcggttcat ggattcggtt
     9781 agatgggaaa ccgccatcag taccaggtcg taatcccaca cactggccat gccggccggc
     9841 cctgcggaaa cctctacgtg cccgtctgga agctcgtagc ggatcacctc gccagctcgt
     9901 cggtcacgct tcgacagacg gaaaacggcc acgtccatga tgctgcgact atcgcgggtg
     9961 cccacgtcat agagcatcgg aacgaaaaaa tctggttgct cgtcgccctt gggcggcttc
    10021 ctaatcgacg gcgcaccggc tgccggcggt tgccgggatt ctttgcggat tcgatcagcg
    10081 gccgcttgcc acgattcacc ggggcgtgct tctgcctcga tgcgttgccg ctgggcggcc
    10141 tgcgcggcct tcaacttctc caccaggtca tcacccagcg ccgcgccgat ttgtaccggg
    10201 ccggatggtt tgcgaccgct cacgccgatt cctcgggctt gggggttcca gtgccattgc
    10261 agggccggca gacaacccag ccgcttacgc ctggccaacc gcccgttcct ccacacatgg
    10321 ggcattccac ggcgtcggtg cctggttgtt cttgattttc catgccgcct cctttagccg
    10381 ctaaaattca tctactcatt tattcatttg ctcatttact ctggtagctg cgcgatgtat
    10441 tcagatagca gctcggtaat ggtcttgcct tggcgtaccg cgtacatctt cagcttggtg
    10501 tgatcctccg ccggcaactg aaagttgacc cgcttcatgg ctggcgtgtc tgccaggctg
    10561 gccaacgttg cagccttgct gctgcgtgcg ctcggacggc cggcacttag cgtgtttgtg
    10621 cttttgctca ttttctcttt acctcattaa ctcaaatgag ttttgattta atttcagcgg
    10681 ccagcgcctg gacctcgcgg gcagcgtcgc cctcgggttc tgattcaaga acggttgtgc
    10741 cggcggcggc agtgcctggg tagctcacgc gctgcgtgat acgggactca agaatgggca
    10801 gctcgtaccc ggccagcgcc tcggcaacct caccgccgat gcgcgtgcct ttgatcgccc
    10861 gcgacacgac aaaggccgct tgtagccttc catccgtgac ctcaatgcgc tgcttaacca
    10921 gctccaccag gtcggcggtg gcccatatgt cgtaagggct tggctgcacc ggaatcagca
    10981 cgaagtcggc tgccttgatc gcggacacag ccaagtccgc cgcctggggc gctccgtcga
    11041 tcactacgaa gtcgcgccgg ccgatggcct tcacgtcgcg gtcaatcgtc gggcggtcga
    11101 tgccgacaac ggttagcggt tgatcttccc gcacggccgc ccaatcgcgg gcactgccct
    11161 ggggatcgga atcgactaac agaacatcgg ccccggcgag ttgcagggcg cgggctagat
    11221 gggttgcgat ggtcgtcttg cctgacccgc ctttctggtt aagtacagcg ataaccttca
    11281 tgcgttcccc ttgcgtattt gtttatttac tcatcgcatc atatacgcag cgaccgcatg
    11341 acgcaagctg ttttactcaa atacacatca cctttttaga cggcggcgct cggtttcttc
    11401 agcggccaag ctggccggcc aggccgccag cttggcatca gacaaaccgg ccaggatttc
    11461 atgcagccgc acggttgaga cgtgcgcggg cggctcgaac acgtacccgg ccgcgatcat
    11521 ctccgcctcg atctcttcgg taatgaaaaa cggttcgtcc tggccgtcct ggtgcggttt
    11581 catgcttgtt cctcttggcg ttcattctcg gcggccgcca gggcgtcggc ctcggtcaat
    11641 gcgtcctcac ggaaggcacc gcgccgcctg gcctcggtgg gcgtcacttc ctcgctgcgc
    11701 tcaagtgcgc ggtacagggt cgagcgatgc acgccaagca gtgcagccgc ctctttcacg
    11761 gtgcggcctt cctggtcgat cagctcgcgg gcgtgcgcga tctgtgccgg ggtgagggta
    11821 gggcgggggc caaacttcac gcctcgggcc ttggcggcct cgcgcccgct ccgggtgcgg
    11881 tcgatgatta gggaacgctc gaactcggca atgccggcga acacggtcaa caccatgcgg
    11941 ccggccggcg tggtggtgtc ggcccacggc tctgccaggc tacgcaggcc cgcgccggcc
    12001 tcctggatgc gctcggcaat gtccagtagg tcgcgggtgc tgcgggccag gcggtctagc
    12061 ctggtcactg tcacaacgtc gccagggcgt aggtggtcaa gcatcctggc cagctccggg
    12121 cggtcgcgcc tggtgccggt gatcttctcg gaaaacagct tggtgcagcc ggccgcgtgc
    12181 agttcggccc gttggttggt caagtcctgg tcgtcggtgc tgacgcgggc atagcccagc
    12241 aggccagcgg cggcgctctt gttcatggcg taatgtctcc ggttctagtc gcaagtattc
    12301 tactttatgc gactaaaaca cgcgacaaga aaacgccagg aaaagggcag ggcggcagcc
    12361 tgtcgcgtaa cttaggactt gtgcgacatg tcgttttcag aagacggctg cactgaacgt
    12421 cagaagccga ctgcactata gcagcggagg ggttggatca aagtactttg atcccgaggg
    12481 gaaccctgtg gttggcatgc acatacaaat ggacgaacgg ataaacct
//
