LOCUS       sequence-122375-       14590 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14590)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 14590)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB336
FEATURES             Location/Qualifiers
     source          1..14590
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2044..2733
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDMALK
                     LVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGH"
     protein_bind    2749..2873
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2910..2915,2916..2933,2934..2940)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2994..3653
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3060..3079)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3995..4300
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4179..4198
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(4341..4465)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      4497..4749
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4783..4799)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4783..4799)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4796..4818)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4807..4823
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4831..4837,4838..4855,4856..4861))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4876..4897
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    4982..5157
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(7241..7917)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(7284..7304)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    8112..8136
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             8657..9448
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      9694..10282
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10183..10202
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10436..10453
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    10468..10608
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(10534..10553)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10694..10716
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      10952..11146
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(11212..12285)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(12714..13343)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttcaaa tgagtcacac acattgttga gagacatatc aaaataactc aattgtaagg
      361 taagttgata gatttcccag ttaaatgcat tattttaaca atatttgtat ctcaaatttt
      421 caaaatcata tcaaatttgc agatcttgat gatgattcac aactctgtct tcgatttttg
      481 attgttaacg ggtatcgata tttgggtaac ttaaaaagaa tttgctaaag atcttaaaaa
      541 acagattaaa aaaacctttc aaaatagcta atcctttaaa tccgtttcac aatataatgc
      601 tcattgatga taatcgaaaa gattccattt taaagtgcat ataaattgct taattatctc
      661 tttctgtctc gtaatatttt gttctctcct attggatcgg acccgcatct tctcgatcag
      721 ttcactatac taatatttgg tcaacttcaa gagagattag aacttcgttc tccaaagtgt
      781 aaaggccgac aagcagaaaa ggaaacttca atcagaagcc ttctaggttt tcccgcgtcc
      841 caaaaagcga agggaggacg gggaggacgg tgaggccaga agaagaaagc aacagtaaaa
      901 tctcatgtga ttgcgtgcat gtcatggatc tatgggatgc tagtccaccg tttggaagta
      961 tgtgcagaag gagaaaggcg cgggaggcca aaagcgactt cggccacctc tctactttct
     1021 agaaagtttg ctcgcagctt gtaataagcg cggaaaaggt actcgaaaaa ccggagcaat
     1081 cggtgtggta tcagcagtcg ggttcgctaa acctacgtct tcttattttg gaatgttaaa
     1141 ttgaaagggc gtgccggccc taagcacgga cgatgtgaca gacggaggag tgtgagcaat
     1201 tcggatgacg atgctggccg gcagtcacga caaagagtcc gaaggggcat ggacagggca
     1261 atatataaat ggaaaagagg ctttgaatat tcctaaccct tcgtcgtcgg cctcccaact
     1321 tgccctcgca tgctgaattg gccgcctatc tgcgaatggc cggccgccga ccctaagtcg
     1381 caataacttg aacggagccg atgcaaagtc cttatcgaac gctcgttatt ataaacgggg
     1441 cagacctagg tatttaatta tatcaaaacc ctccgcaatt tcgctaatca ttccctaatt
     1501 tcttctactt tggcgtctag tgctgtggtc gcgcttgctc tcaaagctcg ctgggaggca
     1561 gtggagcgtc tggcttaaaa tcgatcgccg caaccctaga gttcctttca cttctttctc
     1621 tcttgcgtgg agagcatcga catcgaagag ctgcatcttg tcttctggta tgcgccttgt
     1681 gccctcgtct ctgtacgtcg ttacgattgc tcgatattgt gattgtctag acctgaaacc
     1741 cgactggagc ttttacgtgg tgtcgattgg ggcctcgagc gagtgatttt ttaggttcgt
     1801 tttagccaag tacatcccac tttggtcagt cctgttatct cggtaaggtg ttcagttcgg
     1861 tctctggtgc ggaggtgcct cgtaagtttc ctgcatgata gatttgtcta tccttgactt
     1921 gaagaggagt cgaaggtcag cgaggttggc tgtgtgctat ggacgtcggt gctccttggt
     1981 tccgtgcttc tatgggtgtt gcctcaacag tattgatgtg cctgcagaaa ggttgagctt
     2041 acaatgagcg agctgattaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg
     2101 aacaaccacc acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag
     2161 accatgagaa tcaaggtggt cgagggcggc cctctcccct tcgccttcga catcctggct
     2221 accagcttca tgtacggcag cagaaccttc atcaaccaca cccagggcat ccccgacttc
     2281 tttaagcagt ccttccctga gggcttcaca tgggagagag tcaccacata cgaagacggg
     2341 ggcgtgctga ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc
     2401 aagatcagag gggtgaactt cccatccaac ggccctgtga tgcagaagaa aacactcggc
     2461 tgggaggcca acaccgagat gctgtacccc gctgacggcg gcctggaagg cagaagcgac
     2521 atggccctga agctcgtggg cgggggccac ctgatctgca acttcaagac cacatacaga
     2581 tccaagaaac ccgctaagaa cctcaagatg cccggcgtct actatgtgga ccacagactg
     2641 gaaagaatca aggaggccga caaagagacc tacgtcgagc agcacgaggt ggctgtggcc
     2701 agatactgcg acctccctag caaactgggg cacaagctag agttatcaac aagtttgtac
     2761 aaaaaagctg aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg
     2821 cataaaaaac agactacata atactgtaaa acacaacata tccagtcact atggcggccc
     2881 ctcgaggggc cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg
     2941 tgtggatttt gagttaggat ccggcgagat tttcaggagc taaggaagct aaaatggaga
     3001 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg
     3061 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg
     3121 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc
     3181 ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg
     3241 tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt
     3301 catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag
     3361 atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt
     3421 ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata
     3481 tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg
     3541 tgctgatgcc gctggcgatt caggttcatc atgccgtctg tgatggcttc catgtcggca
     3601 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg taaagatctg
     3661 gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta
     3721 taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtgtg ctatgaagca
     3781 gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc
     3841 aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa
     3901 cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac
     3961 ggctcttttg ctgacgagaa cagggactgg tgaaatgcag tttaaggttt acacctataa
     4021 aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg
     4081 gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga
     4141 actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc
     4201 cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga
     4261 catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca
     4321 cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag
     4381 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
     4441 gttcagcttt cttgtacaaa gtggttgata acagcgctta gagctcgaat ttccccgatc
     4501 gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga
     4561 ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga
     4621 cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga
     4681 tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt
     4741 tactagatcg ggaattggtt ccggaaccaa ttcgtaatca tggtcatagc tgtttcctgt
     4801 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     4861 agcctggggt gcctaatgag tgagctaact cacattaggc tgaattaggc gcgcctattt
     4921 ctgaattcaa cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg
     4981 ggatctggat tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt
     5041 attttacaaa tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc
     5101 ctataggaac cctaaattcc cttatctggg aactactcac acattattat ggagaaactc
     5161 gaagttagta tatagtcctt ccatcaccct ctgtgataat ctccttaaaa cttgctcccc
     5221 caggaatcat aggcagaacg tgctcctggt ggggaactat cacatccagt aaataaggac
     5281 cgggagaatc tagcatagtt tgaattgctg ctcttagttc atccttcttc accacacgcg
     5341 catttggcac cttgaaaccg tctgcaactg tacagaagtc ggggtagatc tgctcctcgt
     5401 gatctggatc acctaagtaa gtatgtgctc tgttggcctt gtagaatcta tcctccaatt
     5461 gtacgaccat tcctagatgt tggttgttta agatcatgat cttcacgggt aacttctcca
     5521 caaaaattgt agccaactcc tgtacgttca tcacaaagct accatcgcca tcgatgtcga
     5581 tcacaggaat atctggttga gttgcggcag cccccaaagc agaaggaagt ccgaatccca
     5641 tggctccaag acctccagat gtcaaccaac gccgcggttc gttgtacttg aaccactggg
     5701 ctgcccacat ttgatgttgg ccaacaccag tagatatgat ggcatttcca ccagtgagtt
     5761 cgtacagcgt ctgtatggca gattgaggag caatggcatc ctcgaatacg ggaaacttca
     5821 ttggccattt ctgcttttgt tcttctagtt ctgccaccca gttggagaag tcgaaagtcc
     5881 tcttttgact cttcaagagg gcattgattt cacttagcgc aggtttcaca tcagcacaca
     5941 tggacacatg gggctgtttg ttctttccaa tttcagcagg gtcaatatcg atatgaacga
     6001 tgctggacct gctagcaaaa gtctccagtt taccagtcac tcgatcgtcg aaacgcactc
     6061 caaatgccaa caacagatct gcttggtcca cggcgtagtt ggcgtaaaca gttccatgca
     6121 ttccaagcat tttcaaactt aatggatcag actcgggata cgctcccaaa cccattaaag
     6181 tcgatgtgat aggaattccg gtaagctcga caaattccct caactcttcg ctggcgtgat
     6241 tgcaacctcc tccagaatac actacaggct tcttagaact agaaatgagt cttataatct
     6301 gctcgattaa cgttggttcc ggtggccgag gcagtcgttg taagtagccc ttcagttgca
     6361 ttggctgaga ccaatctgga atagccattt gctgttgcac gtccttggga atatctacca
     6421 agactgggcc cggcctacct gaagctgcca aaaagaaagc ctccctgatt acgcgaggga
     6481 tgtcatcaat gttcataaca aggtaattgt gcttggtgat cgatctcgta acctccacaa
     6541 ttggagtttc ttgaaaagca tcggtaccaa tcatcctact ggatacttga cctgtaatgg
     6601 ccactagtgg cacactgtcc aagagggcat cagcgagccc ggtaacaaga ttggtcgctc
     6661 caggaccgga tgtggctata caaaccccta ctcgtccaga cgatttagca taaccttcag
     6721 cagcgaaaat ctccccctgc tcgtggcgac agagaatgtt gcggatgctg ctggaacgtg
     6781 taagagcctg gtgaatctcc atagacgctc cgccgggata agcgaacacc gttctaatcc
     6841 cttctcgctc aagagcctcc accaaaatgt cggctccttt gcggggctcg tcatccttgt
     6901 agcgagactg aaagtcggac ggggttggtg caacgagtga cttcccgctg ctaagccgtg
     6961 gtccactcac atcgactgca gactggatct tctcgctaac agcttgggct ttcacttcca
     7021 gtgaacgctc agtcttccga gaggtgttgt atgcatgtcc cctcagagcg gctcccgaga
     7081 agaaaccact cctcaccttt agctctttag ctcccttgca acaagacctt gccgatggct
     7141 gcaccttctc caggctcgct atgctcaaag gcacagctgc gcatccacta attgacgcca
     7201 tgctcgagag agatagattt gtagagagag actggtgatt tcagcgtgtc ctctccaaat
     7261 gaaatgaact tccttatata gaggaagggt cttgcgaagg atagtgggat tgtgcgtcat
     7321 cccttacgtc agtggagata tcacatcaat ccacttgctt tgaagacgtg gttggaacgt
     7381 cttctttttc cacgatgctc ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag
     7441 aggcatcttg aacgatagcc tttcctttat cgcaattatg gcatttgtag gtgccacctt
     7501 ccttttctac tgtccttttg atgaagtgac agatagctgg gcaatggaat ccgaggaggt
     7561 ttcccgatat taccctttgt tgaaaagtct caatagccct ttggtcttct gagactgtat
     7621 ctttgatatt cttggagtag acgagagtgt cgtgctccac catgttcaca tcaatccact
     7681 tgctttgaag acgtggttgg aacgtcttct ttttccacga tgctcctcgt gggtgggggt
     7741 ccatctttgg gaccactgtc ggcagaggca tcttgaacga tagcctttcc tttatcgcaa
     7801 tgatggcatt tgtaggtgcc accttccttt tctactgtcc ttttgatgaa gtgacagata
     7861 gctgggcaat ggaatccgag gaggtttccc gatattaccc tttgttgaaa agtctcaata
     7921 gccctttggt cttctgagac tgtatctttg atattcttgg agtagacgag agtgtcgtgc
     7981 tccaccatgt tggcaagctg ctctagccaa tacgcaaacc gaattcagaa ataaattcag
     8041 cctaattcgg cgttaattca gtacattaaa aacgtccgca atgtgttatt aagttgtcta
     8101 agcgtcaatt tgtttacacc acaatatatc ctgccaccag ccagccaaca gctccccgac
     8161 cggcagctcg gcacaaaatc accactcgat acaggcagcc catcagtccg ggacggcgtc
     8221 agcgggagag ccgttgtaag gcggcagact ttgctcatgt taccgatgct attcggaaga
     8281 acggcaacta agctgccggg tttgaaacac ggatgatctc gcggagggta gcatgttgat
     8341 tgtaacgatg acagagcgtt gctgcctgtg atcaattcgg gcacgaaccc agtggacata
     8401 agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca actggtccag
     8461 aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga
     8521 ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt
     8581 gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa
     8641 aacaaagtta aacatcatgg gggaagcggt gatcgccgaa gtatcgactc aactatcaga
     8701 ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg
     8761 ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac
     8821 cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc
     8881 ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga
     8941 catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa
     9001 tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct
     9061 gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga
     9121 tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc
     9181 gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta
     9241 cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg
     9301 cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga
     9361 agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg
     9421 cgagatcacc aaggtagtcg gcaaataatg tctagctaga aattcgttca agccgacgcc
     9481 gcttcgcggc gcggcttaac tcaagcgtta gatgcactaa gcacataatt gctcacagcc
     9541 aaactatcag gtcaagtctg cttttattat ttttaagcgt gcataataag ccctacacaa
     9601 attgggagat atatcatgca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     9661 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     9721 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     9781 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     9841 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     9901 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     9961 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
    10021 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
    10081 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
    10141 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
    10201 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
    10261 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
    10321 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
    10381 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
    10441 gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg
    10501 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
    10561 aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg
    10621 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
    10681 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
    10741 gcgaggcagg gtgccttgat gtgggcgccg gcggtcgagt ggcgacggcg cggcttgtcc
    10801 gcgccctggt agattgcctg gccgtaggcc agccattttt gagcggccag cggccgcgat
    10861 aggccgacgc gaagcggcgg ggcgtaggga gcgcagcgac cgaagggtag gcgctttttg
    10921 cagctcttcg gctgtgcgct ggccagacag ttatgcacag gccaggcggg ttttaagagt
    10981 tttaataagt tttaaagagt tttaggcgga aaaatcgcct tttttctctt ttatatcagt
    11041 cacttacatg tgtgaccggt tcccaatgta cggctttggg ttcccaatgt acgggttccg
    11101 gttcccaatg tacggctttg ggttcccaat gtacgtgcta tccacaggaa agagaccttt
    11161 tcgacctttt tcccctgcta gggcaatttg ccctagcatc tgctccgtac attaggaacc
    11221 ggcggatgct tcgccctcga tcaggttgcg gtagcgcatg actaggatcg ggccagcctg
    11281 ccccgcctcc tccttcaaat cgtactccgg caggtcattt gacccgatca gcttgcgcac
    11341 ggtgaaacag aacttcttga actctccggc gctgccactg cgttcgtaga tcgtcttgaa
    11401 caaccatctg gcttctgcct tgcctgcggc gcggcgtgcc aggcggtaga gaaaacggcc
    11461 gatgccggga tcgatcaaaa agtaatcggg gtgaaccgtc agcacgtccg ggttcttgcc
    11521 ttctgtgatc tcgcggtaca tccaatcagc tagctcgatc tcgatgtact ccggccgccc
    11581 ggtttcgctc tttacgatct tgtagcggct aatcaaggct tcaccctcgg ataccgtcac
    11641 caggcggccg ttcttggcct tcttcgtacg ctgcatggca acgtgcgtgg tgtttaaccg
    11701 aatgcaggtt tctaccaggt cgtctttctg ctttccgcca tcggctcgcc ggcagaactt
    11761 gagtacgtcc gcaacgtgtg gacggaacac gcggccgggc ttgtctccct tcccttcccg
    11821 gtatcggttc atggattcgg ttagatggga aaccgccatc agtaccaggt cgtaatccca
    11881 cacactggcc atgccggccg gccctgcgga aacctctacg tgcccgtctg gaagctcgta
    11941 gcggatcacc tcgccagctc gtcggtcacg cttcgacaga cggaaaacgg ccacgtccat
    12001 gatgctgcga ctatcgcggg tgcccacgtc atagagcatc ggaacgaaaa aatctggttg
    12061 ctcgtcgccc ttgggcggct tcctaatcga cggcgcaccg gctgccggcg gttgccggga
    12121 ttctttgcgg attcgatcag cggccgcttg ccacgattca ccggggcgtg cttctgcctc
    12181 gatgcgttgc cgctgggcgg cctgcgcggc cttcaacttc tccaccaggt catcacccag
    12241 cgccgcgccg atttgtaccg ggccggatgg tttgcgaccg ctcacgccga ttcctcgggc
    12301 ttgggggttc cagtgccatt gcagggccgg cagacaaccc agccgcttac gcctggccaa
    12361 ccgcccgttc ctccacacat ggggcattcc acggcgtcgg tgcctggttg ttcttgattt
    12421 tccatgccgc ctcctttagc cgctaaaatt catctactca tttattcatt tgctcattta
    12481 ctctggtagc tgcgcgatgt attcagatag cagctcggta atggtcttgc cttggcgtac
    12541 cgcgtacatc ttcagcttgg tgtgatcctc cgccggcaac tgaaagttga cccgcttcat
    12601 ggctggcgtg tctgccaggc tggccaacgt tgcagccttg ctgctgcgtg cgctcggacg
    12661 gccggcactt agcgtgtttg tgcttttgct cattttctct ttacctcatt aactcaaatg
    12721 agttttgatt taatttcagc ggccagcgcc tggacctcgc gggcagcgtc gccctcgggt
    12781 tctgattcaa gaacggttgt gccggcggcg gcagtgcctg ggtagctcac gcgctgcgtg
    12841 atacgggact caagaatggg cagctcgtac ccggccagcg cctcggcaac ctcaccgccg
    12901 atgcgcgtgc ctttgatcgc ccgcgacacg acaaaggccg cttgtagcct tccatccgtg
    12961 acctcaatgc gctgcttaac cagctccacc aggtcggcgg tggcccatat gtcgtaaggg
    13021 cttggctgca ccggaatcag cacgaagtcg gctgccttga tcgcggacac agccaagtcc
    13081 gccgcctggg gcgctccgtc gatcactacg aagtcgcgcc ggccgatggc cttcacgtcg
    13141 cggtcaatcg tcgggcggtc gatgccgaca acggttagcg gttgatcttc ccgcacggcc
    13201 gcccaatcgc gggcactgcc ctggggatcg gaatcgacta acagaacatc ggccccggcg
    13261 agttgcaggg cgcgggctag atgggttgcg atggtcgtct tgcctgaccc gcctttctgg
    13321 ttaagtacag cgataacctt catgcgttcc ccttgcgtat ttgtttattt actcatcgca
    13381 tcatatacgc agcgaccgca tgacgcaagc tgttttactc aaatacacat caccttttta
    13441 gacggcggcg ctcggtttct tcagcggcca agctggccgg ccaggccgcc agcttggcat
    13501 cagacaaacc ggccaggatt tcatgcagcc gcacggttga gacgtgcgcg ggcggctcga
    13561 acacgtaccc ggccgcgatc atctccgcct cgatctcttc ggtaatgaaa aacggttcgt
    13621 cctggccgtc ctggtgcggt ttcatgcttg ttcctcttgg cgttcattct cggcggccgc
    13681 cagggcgtcg gcctcggtca atgcgtcctc acggaaggca ccgcgccgcc tggcctcggt
    13741 gggcgtcact tcctcgctgc gctcaagtgc gcggtacagg gtcgagcgat gcacgccaag
    13801 cagtgcagcc gcctctttca cggtgcggcc ttcctggtcg atcagctcgc gggcgtgcgc
    13861 gatctgtgcc ggggtgaggg tagggcgggg gccaaacttc acgcctcggg ccttggcggc
    13921 ctcgcgcccg ctccgggtgc ggtcgatgat tagggaacgc tcgaactcgg caatgccggc
    13981 gaacacggtc aacaccatgc ggccggccgg cgtggtggtg tcggcccacg gctctgccag
    14041 gctacgcagg cccgcgccgg cctcctggat gcgctcggca atgtccagta ggtcgcgggt
    14101 gctgcgggcc aggcggtcta gcctggtcac tgtcacaacg tcgccagggc gtaggtggtc
    14161 aagcatcctg gccagctccg ggcggtcgcg cctggtgccg gtgatcttct cggaaaacag
    14221 cttggtgcag ccggccgcgt gcagttcggc ccgttggttg gtcaagtcct ggtcgtcggt
    14281 gctgacgcgg gcatagccca gcaggccagc ggcggcgctc ttgttcatgg cgtaatgtct
    14341 ccggttctag tcgcaagtat tctactttat gcgactaaaa cacgcgacaa gaaaacgcca
    14401 ggaaaagggc agggcggcag cctgtcgcgt aacttaggac ttgtgcgaca tgtcgttttc
    14461 agaagacggc tgcactgaac gtcagaagcc gactgcacta tagcagcgga ggggttggat
    14521 caaagtactt tgatcccgag gggaaccctg tggttggcat gcacatacaa atggacgaac
    14581 ggataaacct
//
