LOCUS       sequence-122376-       13422 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13422)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 13422)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB436
FEATURES             Location/Qualifiers
     source          1..13422
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2044..2733
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDMALK
                     LVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGH"
     protein_bind    2749..2873
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2910..2915,2916..2933,2934..2940)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2994..3653
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3060..3079)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3995..4300
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4179..4198
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(4341..4465)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      4497..4749
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4783..4799)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4783..4799)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4796..4818)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4807..4823
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4831..4837,4838..4855,4856..4861))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4876..4897
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    4982..5156
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(5213..6010)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5324..5343)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5934..5953
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(6073..6749)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(6116..6136)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    6944..6968
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7489..8280
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8526..9114
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9015..9034
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9268..9285
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9300..9440
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9366..9385)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9526..9548
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9784..9978
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10044..11117)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11546..12175)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttcaaa tgagtcacac acattgttga gagacatatc aaaataactc aattgtaagg
      361 taagttgata gatttcccag ttaaatgcat tattttaaca atatttgtat ctcaaatttt
      421 caaaatcata tcaaatttgc agatcttgat gatgattcac aactctgtct tcgatttttg
      481 attgttaacg ggtatcgata tttgggtaac ttaaaaagaa tttgctaaag atcttaaaaa
      541 acagattaaa aaaacctttc aaaatagcta atcctttaaa tccgtttcac aatataatgc
      601 tcattgatga taatcgaaaa gattccattt taaagtgcat ataaattgct taattatctc
      661 tttctgtctc gtaatatttt gttctctcct attggatcgg acccgcatct tctcgatcag
      721 ttcactatac taatatttgg tcaacttcaa gagagattag aacttcgttc tccaaagtgt
      781 aaaggccgac aagcagaaaa ggaaacttca atcagaagcc ttctaggttt tcccgcgtcc
      841 caaaaagcga agggaggacg gggaggacgg tgaggccaga agaagaaagc aacagtaaaa
      901 tctcatgtga ttgcgtgcat gtcatggatc tatgggatgc tagtccaccg tttggaagta
      961 tgtgcagaag gagaaaggcg cgggaggcca aaagcgactt cggccacctc tctactttct
     1021 agaaagtttg ctcgcagctt gtaataagcg cggaaaaggt actcgaaaaa ccggagcaat
     1081 cggtgtggta tcagcagtcg ggttcgctaa acctacgtct tcttattttg gaatgttaaa
     1141 ttgaaagggc gtgccggccc taagcacgga cgatgtgaca gacggaggag tgtgagcaat
     1201 tcggatgacg atgctggccg gcagtcacga caaagagtcc gaaggggcat ggacagggca
     1261 atatataaat ggaaaagagg ctttgaatat tcctaaccct tcgtcgtcgg cctcccaact
     1321 tgccctcgca tgctgaattg gccgcctatc tgcgaatggc cggccgccga ccctaagtcg
     1381 caataacttg aacggagccg atgcaaagtc cttatcgaac gctcgttatt ataaacgggg
     1441 cagacctagg tatttaatta tatcaaaacc ctccgcaatt tcgctaatca ttccctaatt
     1501 tcttctactt tggcgtctag tgctgtggtc gcgcttgctc tcaaagctcg ctgggaggca
     1561 gtggagcgtc tggcttaaaa tcgatcgccg caaccctaga gttcctttca cttctttctc
     1621 tcttgcgtgg agagcatcga catcgaagag ctgcatcttg tcttctggta tgcgccttgt
     1681 gccctcgtct ctgtacgtcg ttacgattgc tcgatattgt gattgtctag acctgaaacc
     1741 cgactggagc ttttacgtgg tgtcgattgg ggcctcgagc gagtgatttt ttaggttcgt
     1801 tttagccaag tacatcccac tttggtcagt cctgttatct cggtaaggtg ttcagttcgg
     1861 tctctggtgc ggaggtgcct cgtaagtttc ctgcatgata gatttgtcta tccttgactt
     1921 gaagaggagt cgaaggtcag cgaggttggc tgtgtgctat ggacgtcggt gctccttggt
     1981 tccgtgcttc tatgggtgtt gcctcaacag tattgatgtg cctgcagaaa ggttgagctt
     2041 acaatgagcg agctgattaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg
     2101 aacaaccacc acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag
     2161 accatgagaa tcaaggtggt cgagggcggc cctctcccct tcgccttcga catcctggct
     2221 accagcttca tgtacggcag cagaaccttc atcaaccaca cccagggcat ccccgacttc
     2281 tttaagcagt ccttccctga gggcttcaca tgggagagag tcaccacata cgaagacggg
     2341 ggcgtgctga ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc
     2401 aagatcagag gggtgaactt cccatccaac ggccctgtga tgcagaagaa aacactcggc
     2461 tgggaggcca acaccgagat gctgtacccc gctgacggcg gcctggaagg cagaagcgac
     2521 atggccctga agctcgtggg cgggggccac ctgatctgca acttcaagac cacatacaga
     2581 tccaagaaac ccgctaagaa cctcaagatg cccggcgtct actatgtgga ccacagactg
     2641 gaaagaatca aggaggccga caaagagacc tacgtcgagc agcacgaggt ggctgtggcc
     2701 agatactgcg acctccctag caaactgggg cacaagctag agttatcaac aagtttgtac
     2761 aaaaaagctg aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg
     2821 cataaaaaac agactacata atactgtaaa acacaacata tccagtcact atggcggccc
     2881 ctcgaggggc cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg
     2941 tgtggatttt gagttaggat ccggcgagat tttcaggagc taaggaagct aaaatggaga
     3001 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg
     3061 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg
     3121 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc
     3181 ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg
     3241 tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt
     3301 catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag
     3361 atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt
     3421 ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata
     3481 tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg
     3541 tgctgatgcc gctggcgatt caggttcatc atgccgtctg tgatggcttc catgtcggca
     3601 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg taaagatctg
     3661 gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta
     3721 taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtgtg ctatgaagca
     3781 gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc
     3841 aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa
     3901 cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac
     3961 ggctcttttg ctgacgagaa cagggactgg tgaaatgcag tttaaggttt acacctataa
     4021 aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg
     4081 gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga
     4141 actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc
     4201 cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga
     4261 catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca
     4321 cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag
     4381 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
     4441 gttcagcttt cttgtacaaa gtggttgata acagcgctta gagctcgaat ttccccgatc
     4501 gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga
     4561 ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga
     4621 cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga
     4681 tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt
     4741 tactagatcg ggaattggtt ccggaaccaa ttcgtaatca tggtcatagc tgtttcctgt
     4801 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     4861 agcctggggt gcctaatgag tgagctaact cacattaggc tgaattaggc gcgcctattt
     4921 ctgaattcaa cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg
     4981 ggatctggat tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt
     5041 attttacaaa tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc
     5101 ctataggaac cctaattccc ttatctggga actactcaca cattattatg gagaaactcg
     5161 agcttgtcga tcgactctag ctagaggatc gatccgaacc ccagagtccc gctcagaaga
     5221 actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa
     5281 gcacgaggaa gcggtcagcc cattcgccgc caagctcttc agcaatatca cgggtagcca
     5341 acgctatgtc ctgatagcgg tccgccacac ccagccggcc acagtcgatg aatccagaaa
     5401 agcggccatt ttccaccatg atattcggca agcaggcatc gccatgtgtc acgacgagat
     5461 cctcgccgtc gggcatgcgc gccttgagcc tggcgaacag ttcggctggc gcgagcccct
     5521 gatgctcttc gtccagatca tcctgatcga caagaccggc ttccatccga gtacgtgctc
     5581 gctcgatgcg atgtttcgct tggtggtcga atgggcaggt agccggatca agcgtatgca
     5641 gccgccgcat tgcatcagcc atgatggata ctttctcggc aggagcaagg tgagatgaca
     5701 ggagatcctg ccccggcact tcgcccaata gcagccagtc ccttcccgct tcagtgacaa
     5761 cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct
     5821 cgtcctggag ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc
     5881 cctgcgctga cagccggaac acggcggcat cagagcagcc gattgtctgt tgtgcccagt
     5941 catagccgaa tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt
     6001 caatccccat ggtcgatcga cagatctgcg aaagctcgag agagatagat ttgtagagag
     6061 agactggtga tttcagcgtg tcctctccaa atgaaatgaa cttccttata tagaggaagg
     6121 gtcttgcgaa ggatagtggg attgtgcgtc atcccttacg tcagtggaga tatcacatca
     6181 atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg
     6241 tgggggtcca tctttgggac cactgtcggc agaggcatct tgaacgatag cctttccttt
     6301 atcgcaatga tggcatttgt aggtgccacc ttccttttct actgtccttt tgatgaagtg
     6361 acagatagct gggcaatgga atccgaggag gtttcccgat attacccttt gttgaaaagt
     6421 ctcaatagcc ctttggtctt ctgagactgt atctttgata ttcttggagt agacgagagt
     6481 gtcgtgctcc accatgttca catcaatcca cttgctttga agacgtggtt ggaacgtctt
     6541 ctttttccac gatgctcctc gtgggtgggg gtccatcttt gggaccactg tcggcagagg
     6601 catcttgaac gatagccttt cctttatcgc aatgatggca tttgtaggtg ccaccttcct
     6661 tttctactgt ccttttgatg aagtgacaga tagctgggca atggaatccg aggaggtttc
     6721 ccgatattac cctttgttga aaagtctcaa tagccctttg gtcttctgag actgtatctt
     6781 tgatattctt ggagtagacg agagtgtcgt gctccaccat gttggcaagc tgctctagcc
     6841 aatacgcaaa ccgaattcag aaataaattc agcctaattc ggcgttaatt cagtacatta
     6901 aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca ccacaatata
     6961 tcctgccacc agccagccaa cagctccccg accggcagct cggcacaaaa tcaccactcg
     7021 atacaggcag cccatcagtc cgggacggcg tcagcgggag agccgttgta aggcggcaga
     7081 ctttgctcat gttaccgatg ctattcggaa gaacggcaac taagctgccg ggtttgaaac
     7141 acggatgatc tcgcggaggg tagcatgttg attgtaacga tgacagagcg ttgctgcctg
     7201 tgatcaattc gggcacgaac ccagtggaca taagcctgtt cggttcgtaa gctgtaatgc
     7261 aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac
     7321 ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttggggtaca gtctatgcct
     7381 cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc
     7441 aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacatcat gggggaagcg
     7501 gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc
     7561 gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca
     7621 cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga
     7681 gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc
     7741 gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag
     7801 cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca
     7861 gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc
     7921 ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag
     7981 gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga
     8041 aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg
     8101 aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata
     8161 cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat
     8221 cagttggaag aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa
     8281 tgtctagcta gaaattcgtt caagccgacg ccgcttcgcg gcgcggctta actcaagcgt
     8341 tagatgcact aagcacataa ttgctcacag ccaaactatc aggtcaagtc tgcttttatt
     8401 atttttaagc gtgcataata agccctacac aaattgggag atatatcatg catgaccaaa
     8461 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     8521 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     8581 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     8641 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     8701 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     8761 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     8821 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     8881 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     8941 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     9001 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     9061 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     9121 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     9181 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     9241 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     9301 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact
     9361 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac
     9421 gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     9481 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     9541 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gggtgccttg atgtgggcgc
     9601 cggcggtcga gtggcgacgg cgcggcttgt ccgcgccctg gtagattgcc tggccgtagg
     9661 ccagccattt ttgagcggcc agcggccgcg ataggccgac gcgaagcggc ggggcgtagg
     9721 gagcgcagcg accgaagggt aggcgctttt tgcagctctt cggctgtgcg ctggccagac
     9781 agttatgcac aggccaggcg ggttttaaga gttttaataa gttttaaaga gttttaggcg
     9841 gaaaaatcgc cttttttctc ttttatatca gtcacttaca tgtgtgaccg gttcccaatg
     9901 tacggctttg ggttcccaat gtacgggttc cggttcccaa tgtacggctt tgggttccca
     9961 atgtacgtgc tatccacagg aaagagacct tttcgacctt tttcccctgc tagggcaatt
    10021 tgccctagca tctgctccgt acattaggaa ccggcggatg cttcgccctc gatcaggttg
    10081 cggtagcgca tgactaggat cgggccagcc tgccccgcct cctccttcaa atcgtactcc
    10141 ggcaggtcat ttgacccgat cagcttgcgc acggtgaaac agaacttctt gaactctccg
    10201 gcgctgccac tgcgttcgta gatcgtcttg aacaaccatc tggcttctgc cttgcctgcg
    10261 gcgcggcgtg ccaggcggta gagaaaacgg ccgatgccgg gatcgatcaa aaagtaatcg
    10321 gggtgaaccg tcagcacgtc cgggttcttg ccttctgtga tctcgcggta catccaatca
    10381 gctagctcga tctcgatgta ctccggccgc ccggtttcgc tctttacgat cttgtagcgg
    10441 ctaatcaagg cttcaccctc ggataccgtc accaggcggc cgttcttggc cttcttcgta
    10501 cgctgcatgg caacgtgcgt ggtgtttaac cgaatgcagg tttctaccag gtcgtctttc
    10561 tgctttccgc catcggctcg ccggcagaac ttgagtacgt ccgcaacgtg tggacggaac
    10621 acgcggccgg gcttgtctcc cttcccttcc cggtatcggt tcatggattc ggttagatgg
    10681 gaaaccgcca tcagtaccag gtcgtaatcc cacacactgg ccatgccggc cggccctgcg
    10741 gaaacctcta cgtgcccgtc tggaagctcg tagcggatca cctcgccagc tcgtcggtca
    10801 cgcttcgaca gacggaaaac ggccacgtcc atgatgctgc gactatcgcg ggtgcccacg
    10861 tcatagagca tcggaacgaa aaaatctggt tgctcgtcgc ccttgggcgg cttcctaatc
    10921 gacggcgcac cggctgccgg cggttgccgg gattctttgc ggattcgatc agcggccgct
    10981 tgccacgatt caccggggcg tgcttctgcc tcgatgcgtt gccgctgggc ggcctgcgcg
    11041 gccttcaact tctccaccag gtcatcaccc agcgccgcgc cgatttgtac cgggccggat
    11101 ggtttgcgac cgctcacgcc gattcctcgg gcttgggggt tccagtgcca ttgcagggcc
    11161 ggcagacaac ccagccgctt acgcctggcc aaccgcccgt tcctccacac atggggcatt
    11221 ccacggcgtc ggtgcctggt tgttcttgat tttccatgcc gcctccttta gccgctaaaa
    11281 ttcatctact catttattca tttgctcatt tactctggta gctgcgcgat gtattcagat
    11341 agcagctcgg taatggtctt gccttggcgt accgcgtaca tcttcagctt ggtgtgatcc
    11401 tccgccggca actgaaagtt gacccgcttc atggctggcg tgtctgccag gctggccaac
    11461 gttgcagcct tgctgctgcg tgcgctcgga cggccggcac ttagcgtgtt tgtgcttttg
    11521 ctcattttct ctttacctca ttaactcaaa tgagttttga tttaatttca gcggccagcg
    11581 cctggacctc gcgggcagcg tcgccctcgg gttctgattc aagaacggtt gtgccggcgg
    11641 cggcagtgcc tgggtagctc acgcgctgcg tgatacggga ctcaagaatg ggcagctcgt
    11701 acccggccag cgcctcggca acctcaccgc cgatgcgcgt gcctttgatc gcccgcgaca
    11761 cgacaaaggc cgcttgtagc cttccatccg tgacctcaat gcgctgctta accagctcca
    11821 ccaggtcggc ggtggcccat atgtcgtaag ggcttggctg caccggaatc agcacgaagt
    11881 cggctgcctt gatcgcggac acagccaagt ccgccgcctg gggcgctccg tcgatcacta
    11941 cgaagtcgcg ccggccgatg gccttcacgt cgcggtcaat cgtcgggcgg tcgatgccga
    12001 caacggttag cggttgatct tcccgcacgg ccgcccaatc gcgggcactg ccctggggat
    12061 cggaatcgac taacagaaca tcggccccgg cgagttgcag ggcgcgggct agatgggttg
    12121 cgatggtcgt cttgcctgac ccgcctttct ggttaagtac agcgataacc ttcatgcgtt
    12181 ccccttgcgt atttgtttat ttactcatcg catcatatac gcagcgaccg catgacgcaa
    12241 gctgttttac tcaaatacac atcacctttt tagacggcgg cgctcggttt cttcagcggc
    12301 caagctggcc ggccaggccg ccagcttggc atcagacaaa ccggccagga tttcatgcag
    12361 ccgcacggtt gagacgtgcg cgggcggctc gaacacgtac ccggccgcga tcatctccgc
    12421 ctcgatctct tcggtaatga aaaacggttc gtcctggccg tcctggtgcg gtttcatgct
    12481 tgttcctctt ggcgttcatt ctcggcggcc gccagggcgt cggcctcggt caatgcgtcc
    12541 tcacggaagg caccgcgccg cctggcctcg gtgggcgtca cttcctcgct gcgctcaagt
    12601 gcgcggtaca gggtcgagcg atgcacgcca agcagtgcag ccgcctcttt cacggtgcgg
    12661 ccttcctggt cgatcagctc gcgggcgtgc gcgatctgtg ccggggtgag ggtagggcgg
    12721 gggccaaact tcacgcctcg ggccttggcg gcctcgcgcc cgctccgggt gcggtcgatg
    12781 attagggaac gctcgaactc ggcaatgccg gcgaacacgg tcaacaccat gcggccggcc
    12841 ggcgtggtgg tgtcggccca cggctctgcc aggctacgca ggcccgcgcc ggcctcctgg
    12901 atgcgctcgg caatgtccag taggtcgcgg gtgctgcggg ccaggcggtc tagcctggtc
    12961 actgtcacaa cgtcgccagg gcgtaggtgg tcaagcatcc tggccagctc cgggcggtcg
    13021 cgcctggtgc cggtgatctt ctcggaaaac agcttggtgc agccggccgc gtgcagttcg
    13081 gcccgttggt tggtcaagtc ctggtcgtcg gtgctgacgc gggcatagcc cagcaggcca
    13141 gcggcggcgc tcttgttcat ggcgtaatgt ctccggttct agtcgcaagt attctacttt
    13201 atgcgactaa aacacgcgac aagaaaacgc caggaaaagg gcagggcggc agcctgtcgc
    13261 gtaacttagg acttgtgcga catgtcgttt tcagaagacg gctgcactga acgtcagaag
    13321 ccgactgcac tatagcagcg gaggggttgg atcaaagtac tttgatcccg aggggaaccc
    13381 tgtggttggc atgcacatac aaatggacga acggataaac ct
//
