LOCUS       sequence-122383-        5946 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes for Ebola Minigenome with luciferase reporter gene under 
            control of a T7 RNA polymerase promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5946)
  AUTHORS   Trunschke M, Conrad D, Enterlein S, Olejnik J, Brauburger K, 
            Muhlberger E
  TITLE     The L-VP35 and L-L interaction domains reside in the amino terminus 
            of the Ebola virus L protein and are potential targets for 
            antivirals.
  JOURNAL   Virology. 2013 Jul 5;441(2):135-45. doi: 
            10.1016/j.virol.2013.03.013. Epub 2013 Apr 11.
  PUBMED    23582637
REFERENCE   2  (bases 1 to 5946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.virol.2013.03.013"; journalName: "Virology"; date: 
            "2013-07-5- 5"; volume: "441"; issue: "2"; pages: "135-45"
COMMENT     Sequence Label: p2,0_3E5E_luciferase
FEATURES             Location/Qualifiers
     source          1..5946
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             479..2131
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(545..563)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     primer_bind     complement(2972..2989)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3143..3731)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3223..3242)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3902..4693,4694..4762))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4525..4544
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4763..4867)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4935..4953
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4991..5013)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5113..5132
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5343..5362
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        5653..5671
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     5653..5670
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     terminator      complement(5739..5786)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     5782..5800
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 cggacacaca aaaagaaaga agaattttta ggatcttttg tgtgcgaata actatgagga
       61 agattaataa ttttcctctc attgaaattt atatcggaat ttaaattgaa attgttactg
      121 taatcacacc tggtttgttt cagagccaca tcacaaagat agagaacaac ctaggtctcc
      181 gaagggagca agggcatcag tgtgctcagt tgaaaatccc ttgtcaacac ctaggtctta
      241 tcacatcaca agttccacct cagactctgc agggtgatcc aacaacctta atagaaacat
      301 tattgttaaa ggacagcatt agttcacagt caaacaagca agattgagaa ttaaccttgg
      361 ttttgaactt gaacacttag gggattgaag attcaacaac cctaaagctt ggggtaaaac
      421 attggaaata gttaaaagac aaattgctcg gaatcacaaa attccgagta tgtctcatat
      481 ggaagacgcc aaaaacataa agaaaggccc ggcgccattc tatccgctgg aagatggaac
      541 cgctggagag caactgcata aggctatgaa gagatacgcc ctggttcctg gaacaattgc
      601 ttttacagat gcacatatcg aggtggacat cacttacgct gagtacttcg aaatgtccgt
      661 tcggttggca gaagctatga aacgatatgg gctgaataca aatcacagaa tcgtcgtatg
      721 cagtgaaaac tctcttcaat tctttatgcc ggtgttgggc gcgttattta tcggagttgc
      781 agttgcgccc gcgaacgaca tttataatga acgtgaattg ctcaacagta tgggcatttc
      841 gcagcctacc gtggtgttcg tttccaaaaa ggggttgcaa aaaattttga acgtgcaaaa
      901 aaagctccca atcatccaaa aaattattat catggattct aaaacggatt accagggatt
      961 tcagtcgatg tacacgttcg tcacatctca tctacctccc ggttttaatg aatacgattt
     1021 tgtgccagag tccttcgata gggacaagac aattgcactg atcatgaact cctctggatc
     1081 tactggtctg cctaaaggtg tcgctctgcc tcatagaact gcctgcgtga gattctcgca
     1141 tgccagagat cctatttttg gcaatcaaat cattccggat actgcgattt taagtgttgt
     1201 tccattccat cacggttttg gaatgtttac tacactcgga tatttgatat gtggatttcg
     1261 agtcgtctta atgtatagat ttgaagaaga gctgtttctg aggagccttc aggattacaa
     1321 gattcaaagt gcgctgctgg tgccaaccct attctccttc ttcgccaaaa gcactctgat
     1381 tgacaaatac gatttatcta atttacacga aattgcttct ggtggcgctc ccctctctaa
     1441 ggaagtcggg gaagcggttg ccaagaggtt ccatctgcca ggtatcaggc aaggatatgg
     1501 gctcactgag actacatcag ctattctgat tacacccgag ggggatgata aaccgggcgc
     1561 ggtcggtaaa gttgttccat tttttgaagc gaaggttgtg gatctggata ccgggaaaac
     1621 gctgggcgtt aatcaaagag gcgaactgtg tgtgagaggt cctatgatta tgtccggtta
     1681 tgtaaacaat ccggaagcga ccaacgcctt gattgacaag gatggatggc tacattctgg
     1741 agacatagct tactgggacg aagacgaaca cttcttcatc gttgaccgcc tgaagtctct
     1801 gattaagtac aaaggctatc aggtggctcc cgctgaattg gaatccatct tgctccaaca
     1861 ccccaacatc ttcgacgcag gtgtcgcagg tcttcccgac gatgacgccg gtgaacttcc
     1921 cgccgccgtt gttgttttgg agcacggaaa gacgatgacg gaaaaagaga tcgtggatta
     1981 cgtcgccagt caagtaacaa ccgcgaaaaa gttgcgcgga ggagttgtgt ttgtggacga
     2041 agtaccgaaa ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga tcctcataaa
     2101 ggccaagaag ggcggaaaga tcgccgtgta agcggccgca tagtatcctg atacttgcaa
     2161 aggttggtta ttaacataca gattataaaa aactcataaa ttgctctcat acatcatatt
     2221 gatctaatct caataaacaa ctatttaaat aacgaaagga gtccctatat tatatactat
     2281 atttagcctc tctccctgcg tgataatcaa aaaattcaca atgcagcatg tgtgacatat
     2341 tactgccgca atgaatttaa cgcaacataa taaactctgc actctttata attaagcttt
     2401 aacgaaaggt ctgggctcat attgttattg atataataat gttgtatcaa tatcctgtca
     2461 gatggaatag tgttttggtt gataacacaa cttcttaaaa caaaattgat ctttaagatt
     2521 aagtttttta taattatcat tactttaatt tgtcgtttta aaaacggtga tagccttaat
     2581 ctttgtgtaa aataagagat taggtgtaat aaccttaaca tttttgtcta gtaagctact
     2641 atttcataca gaatgataaa attaaaagaa aaggcaggac tgtaaaatca gaaatacctt
     2701 ctttacaata tagcagacta gataataatc ttcgtgttaa tgataattaa gacattgacc
     2761 acgctcatca gaaggctcgc cagaataaac gttgcaaaaa ggattcctgg aaaaatggtc
     2821 gcacacaaaa atttaaaaat aaatctattt cttctttttt gtgtgtccac tatagtgagt
     2881 cgtattaacc cgggatcgat atccccctgc attaatgaat cggccaacgc gcggggagag
     2941 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     3001 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     3061 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     3121 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     3181 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     3241 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     3301 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     3361 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     3421 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     3481 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     3541 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     3601 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     3661 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     3721 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     3781 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     3841 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     3901 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     3961 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     4021 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     4081 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     4141 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     4201 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     4261 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     4321 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     4381 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     4441 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     4501 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     4561 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     4621 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     4681 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     4741 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     4801 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     4861 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     4921 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg
     4981 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     5041 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct
     5101 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatc gacgctctcc
     5161 cttatgcgac tcctgcatta agaagcagcc cagtagtagg ttgaggccgt tgagcaccgc
     5221 cgccgcaagg aatggtgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc
     5281 accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca
     5341 tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc
     5401 acgatgcgtc cggcgtagag gatctggcta gcgatgaccc tgctgattgg ttcgctgacc
     5461 atttccgggg tgcggaacgg cgttaccaga aactcagaag gttcgtccaa ccaaaccgac
     5521 tctgacggca gtttacgaga gagatgatag ggtctgcttc agtaagccag atgctacaca
     5581 attaggcttg tacatattgt cgttagaacg cggctacaat taatacataa ccttatgtat
     5641 catacacata cgatttaggt gacactatag aatacaagct tgcatgcctg caggtcgact
     5701 ctagaggatc tcgatccgga tatagttcct cctttcagca aaaaacccct caagacccgt
     5761 ttagaggccc caaggggtta tgctagttat tgctcagcgg tggcagcagc caactcagct
     5821 tcctttcggg ctttgttagc agccggatcc ttttttttga gctctccctt agccatccga
     5881 gtggacgacg tcctccttcg gatgcccagg tcggaccgcg aggaggtgga gatgccatgc
     5941 cgaccc
//
