LOCUS       sequence-122389-       10829 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lgg-1 tagged with oxCerulean-oxVenus (dFP, or double fluorescent 
            protein), expressed in the pharynx, for monitoring autophagy.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10829)
  AUTHORS   Chapin HA, Okada M, Merz AJ, Miller DL
  TITLE     Tissue-specific autophagy responses to aging and stress in C. 
            elegans.
  JOURNAL   Aging (Albany NY). 2015 Jun 30.
  PUBMED    26142908
REFERENCE   2  (bases 1 to 10829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Aging 
            (Albany NY). 2015 Jun 30."
COMMENT     Sequence Label: pMyo-2::oxCerulean-oxVenus::Lgg-1
FEATURES             Location/Qualifiers
     source          1..10829
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(214..233)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     333..355
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(393..411)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        479..583
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(584..652,653..1444)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(802..821)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1615..2203
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2104..2123
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3839..3850)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    5916..5936
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    6916..6940
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7771..7791
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(8543..8567)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9297..9317)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(9325..9342)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9325..9341)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cacgtgacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
       61 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
      121 gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg
      181 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
      241 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
      301 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
      361 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
      421 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
      481 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
      541 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
      601 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
      661 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
      721 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
      781 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
      841 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
      901 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
      961 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     1021 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     1081 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     1141 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     1201 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     1261 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     1321 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     1381 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     1441 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     1501 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     1561 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     1621 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     1681 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     1741 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     1801 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     1861 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     1921 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     1981 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     2041 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     2101 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     2161 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     2221 ctttttacgg ttcctggtct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     2281 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgatacca gcggataaca
     2341 atttcacagg gccctgtcgg attatgggct cttcttttat cctctacttt ctttctagaa
     2401 aatgcaaatg ttgagtcaga aattgctgaa tggagtacaa ctgcaatggg tttagcttat
     2461 gtttctggaa caattactgg tggaattgtg attgaaaagt aaggttatat ttcatttcaa
     2521 taaatgttac cacagtataa caaaaacaag ttacattgtt cagagtatgt ctgaatgtat
     2581 tattattatt gtatttgtgc atcagtactg tagttgtact gaagaagggt ttaaaataat
     2641 ctcaacgttt ttaagacgag ttagtgagaa tacggtcgca gttaggatgt ttggacatag
     2701 ttttattaga taatcgtgaa ctaactcact ataaaaaccc gctaactcat tgaataagat
     2761 agttttaaac gttcaaaatt tgaagacaaa attgtatgaa taagtttaca gagttggtcg
     2821 acgcaagctt ctccttctct tcacatttct caataattta gctctaattg cattcgtatt
     2881 cttcgcaaaa atacgtattc tgattgaccc gatgaaatac ggatgcctag ttgcattgat
     2941 catctacggt tatacttatg ggtgaggttt ttcaaatata ttttgattta atcgaataat
     3001 gaaatttcag aactggagtt ggtccaattt cgtggtttat atcctctgaa ctggtacctc
     3061 aaaaacatcg tagtattgct caatctgtgg catattcaat gaatactgta atggtggtta
     3121 tcagtacatt tacagtatta cctctatatt ccgttattgg tagttatgct ttccttattc
     3181 tatattcaat tccttcattt ataagtatgc ttatattatt tcgctactta ccggaaacca
     3241 aaggacgaga gattcatgat attgtgaatg aacttaaaaa aaaatgaaaa cctttgaaaa
     3301 cgttcatacg gccgaaattt tacttgatcg agccaattga acttttgtta agacttacag
     3361 aaaagtgaga gattttgtta caaaaaagtg agaaaaaagc gaaaatgaac aggaaaaaga
     3421 agaaaaatac acccgaagta tcatataaaa attaaaaaga ttgtttgacc tggcggaact
     3481 tttttgcaaa aactctgaat atccatggca catgcttcgt gcaaaacagg atccaaatga
     3541 atatcaacaa gagattcttg aagacgacga gccacttgct cagagcattg cttatctcga
     3601 atgagaccct tgttaaaatc atttttcaaa cattatatga cattgtcgaa atgtcctcct
     3661 gattccatga tggtagcaaa ctcacttcgt ctttgagcca atttatccaa gtccttgtaa
     3721 aaagtattcg aaaattgtta acggataaaa tgtttattat aatatcaaaa acaatttgtc
     3781 agttgaccac tttttgatat atttgacaga aaccgggatg aattggctca aagttagggc
     3841 gcccttctat tacagggttt ctgataacaa accggttatt aactcccaac aagggatgat
     3901 ttcaattcat catgctcaaa ttgacccaaa ttaagttaca tgacaaattc atcgcccttt
     3961 tcaactcttc ttggctcatc atctgttatt ctgttcctat tctgtctgca ccctataccc
     4021 ctttgcatac tctctcgtca ttcctttctt ggatagtgtc ttcttctccc cagctctgct
     4081 acttctatga cttgcgcccg ctgcttttcc gctcgttcct ctcctctctg acgtcttctt
     4141 ctgcttcttt tctccttcct ttttcccttc tcaatcttcc tctttttccc atttctgtca
     4201 atcattcgaa gaagaagaag aagaagaccc tcattcattt catttttttt tctgtcggtg
     4261 tgtgctgctc ggttaagagt gagctctcta ttccacgtct tcttcttttt cttcttcgat
     4321 tcgaatcaat cactccacca aaacgcattc gtttttggga ttcaccccgc ggttcgcaac
     4381 aggtttcttt ttcaaattat tagcgttata aatagaaaaa tggtggagtt tcaataaaaa
     4441 tgataattta caaagtgtat tttgattaca tgtactcaaa aaggttgaaa tttttcatac
     4501 cagttttccg gaaatccatc tgatatcatt atcgtatttt tctttttaaa aatgttttca
     4561 aaaaaaaaca aaatatagct ggttatttgg caccctctaa ttaccatttt cctgtcacac
     4621 cacactcttt tccttcttcc acttctttcc gttttcagcc gcttccaacc aaaccgatat
     4681 gaaagccgag caacaacaat cgattccacc cggctcggcg accttcccgt cgcaggtgag
     4741 actcagaaaa ctagagaaac cgctcaacta actcttgata tccgatttca ttcttttctc
     4801 ttttcttttt gttgaacttt tccatatttc cagatgccac ggccaccacc aagcaccgaa
     4861 caaggaatca caacggaatc ggagcttgcg aagaaagctc aaatcactcc gaacgacgtt
     4921 ttagcacttc cgggaatcac tcaaggtatc ttgctccctt tggatttcag tttaaaacat
     4981 aaatttagga ttcttatgct ccccatctgc gaacatctat aacatcgagt tcaccaagtt
     5041 ccaaatccgt gatctggaca ctgagcaagt gcttttcgag atcgccaaac cggagaacga
     5101 tcaggagaat gatgagtcgc cacaggagtc ggcaagatac gtgcgttata gatttgctcc
     5161 aaactttttg aaactcaaaa cggtcggagc aactgtggaa ttcaaagtag gagacatccc
     5221 aatccatcat ttccgaatga tcgaacgtca cttcttcaaa gatcgccttc tgaagtgttt
     5281 tgactttgaa ttcggattct gtattccgaa ttcacgaaac aactgtgaac atatctatga
     5341 gttccctcaa ctctctcaac aactcagtga gtcattattc taaaaagtac aattcaaaag
     5401 actaatctct ttttcagtgg acgacatgat caacaatcca aacgagactc gttctgacag
     5461 cttctatttc gtcgataaca aactcgtcat gcacaacaaa gccgactact catatgatgc
     5521 ataaatattt aatacaaaaa tgttctggat aattattctg tcgaatagaa aaaaaactcc
     5581 aaatgtgatt aaattccaat aattcctgtc tagtttgttc cttccccttc cccttctcat
     5641 gttcaatgca ttcctaagct tttcagttcc ccccttgttt ctatattttt tcgctgtcct
     5701 gtcacactcg ctaaaaacac taatcacacg gaaatctgtt ttcaataaaa actccaactt
     5761 taactcattt tcaatttcaa ctgaaagatt ttttcattag agaatgtcta gaactaggcc
     5821 cgggctacgt aatacgactc acttaaggcc ttgactagag ggtaccagag ctcacctagg
     5881 caggaacagc tatgaccatg attacgccaa gctatcaact ttgtatagaa aagttgcatt
     5941 ttatatctga gtagtatcct ttgctttaaa tgtccataaa actaatttta taatcaataa
     6001 aacaacgttt gtaaatcaac tgagtttaca agtagagaca ttgagggata ctttcactat
     6061 gctaaagtga taatcgacca aataataact tcactttggt atttattcct gtcttataaa
     6121 tgttatgtat gaattaaatt catatgcata tggctcactc tgacaatttt taataatctt
     6181 ccagatcaat attgactacc gatgcgggtg gtcttttgct ttgaattctg ctgaacttta
     6241 caccccgaac agcaatgtgt gcttcagcct aaaaaaaagt aagtgtgtta atcagtgccc
     6301 ccgattcttc attttttgcc cctctctccc gtttcgtcgg caaaagaaga gaaaataaag
     6361 ataagtctca agataggttg gtaatcgcta aagtggttgt gtggataaga gtagcaaaat
     6421 ggcaggaaga gcactttgcg cgcacacact gtactcattg ttctggataa aattctctcg
     6481 ttgtttgccg tcggatgtct gcctctctgc attgagccgg cttcttcact atctttagtt
     6541 aacctaaaat gccgtttctt ttctcgtatc cccactatcc cgttgaggtt ctctgctctc
     6601 ttcgctccct accgccagcg agcaactatc cgtgggggcg ccttgctcgg aagatggggg
     6661 ggaagaaaga agatttttgc tatttgcact tgagaaagag acttttcctg cgtcgatggt
     6721 tagagaacag tgtgcagaca cttttcagct acctagaatt acaattggat atccccgcct
     6781 cccaatccac ccacccaggg aaaaagaagg gctcgccgaa aatcaaagtt atctccaggc
     6841 tcgcgcatcc caccgagcgg ttgacttctc tccaccactt ttcattttaa ccctcgatcg
     6901 tcagacacag aaatgacaag tttgtacaaa aaagcaggct caatggtgtc aaagggcgaa
     6961 gagctgttta ctggagtcgt cccaattctg gtggagctgg atggagatgt caacgggcat
     7021 aagttttcag tgcgaggaga gggagaagga gacgctacca acggcaagct gacactgaaa
     7081 ttcatcagca ccacagggaa gctgcccgtg ccttgggtaa gtttaaacat atatatacta
     7141 actaaccctg attatttaaa ttttcagcca accctggtca ctaccctgag ttggggcgtc
     7201 cagtcatttg cccgataccc agaccacatg aagcagcatg atttctttaa atccgctatg
     7261 cccgagggct acgtgcagga acggacaatt ttctttaagg acgatggaac atacaaaact
     7321 agtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc aggagcagag
     7381 gtgaagttcg aaggagacac tctggtcaac aggatcgagc tgaagggcat tgactttaaa
     7441 gaagatggaa atatcctggg ccacaaactg gagtacaacg ccatccatgg caacgtgtac
     7501 attacagctg ataagcagaa aaacgggatt aaggcaaatt tcggactgaa ctgcaatgtg
     7561 gaagacggga gtgtccagct ggccgatcac taccagcaga acactcctat cggcgacggg
     7621 cccgtgctgc tgcctgataa tcattatctg agcacccagt ccgtcctgtc taaggacccc
     7681 aatgagaaaa gagatcacat ggtgctgctg gagtttgtga ccgctgctgg cattaccctg
     7741 gggatggacg agctgtacaa gggaccaggc gagaatttgt attttcaggg tcgtcttgtc
     7801 aagttccttg ttggaggacc aggcatggtg tctaaaggcg aggaactgtt cactggggtg
     7861 gtgcccatcc tggtggagct ggacggcgat gtgaatgggc acaagtttag cgtgcgcgga
     7921 gagggagaag gggacgctac caacggaaag ctgacactga aactgatttc caccacaggc
     7981 aagctgcccg tgccttggcc aacactggtg actaccctgg gatacggcct ccagagcttc
     8041 gcccggtatc cagaccacat gaagcagcat gatttcttta aatcagctat gcccgagggc
     8101 tacgtgcagg aacggacaat cttctttaag gacgatggga catataaaac tagagccgag
     8161 gtgaagtttg aaggggacac tctggtgaac aggatcgagc tgaagggaat tgacttcaaa
     8221 gaagatggaa atatcctggg ccacaagctg gagtacaact tcaacagcca taacgtgtat
     8281 atcaccgccg ataagcagaa aaacggcatc aaggctaatt tcaaaattag gcacaatgtg
     8341 gaagacggcg gggtgcagct ggctgatcat taccagcaga acacacctat cggggacgga
     8401 cccgtgctgc tgcctgataa tcactacctg agctatcagt ccgtgctgtc taaggaccca
     8461 aacgagaaac gggatcatat ggtgctgaaa gaatttgtga ctgccgctgg gattaccctg
     8521 ggaatggatg agctgtataa gcacccagct ttcttgtaca aagtgggaat gaagtgggct
     8581 tacaaggagg agaacaactt tgagaagcgt cgtgccgaag gagacaagat ccgcagaaag
     8641 tacccagacc gtattccagt gattgttgag aaagcaccaa agtcaaagct ccatgacttg
     8701 gataagaaga agtacttggt cccatccgat cttactgttg gacagttcta cttcctcatc
     8761 agaaaacgca tccaacttcg tccagaagat gctctgttct tctttgtcaa caatgtcatt
     8821 ccacaaacca tgaccacaat gggacaactc taccaggtaa ctaaccagtc gtttattttc
     8881 atttaattaa cccttttctt tattacagga ccatcacgag gaagacttgt tcctttacat
     8941 cgcctacagt gacgaaagtg tgtatggagg agaggtcgaa aagaaggaat aaagtgtcat
     9001 gtattatccg ccgacgaatg tgtatacttg tacaatggaa atttcatact tctaatggaa
     9061 acccaaagcc cctttaaaaa aatcttctca ttttctgtaa taattctgta tttccttcac
     9121 attttcttct catttttctc gtatgacggt tccgagtagt gacttctatt caaactattg
     9181 tctacgaagt tgctcttctc ccctcccaga aaatcatgtt ttctctcgga ttttgatatt
     9241 tttttgctat tactttcaaa tttttgattc atgaataaac gaagaagaca caaaaccaac
     9301 tttattatac atagttgata attcactggc cgtcgtttta cactcgagac gtacggtgcg
     9361 cgcgatgcat tcgaagatct gcccactagt gagtcgtatt ataagtgcaa gtaagatcag
     9421 tgtttgtttc gatatcagtc tgtttcgtaa cggtcttctg tataactaca aaaaagaata
     9481 aaaaaccgta tcacaactga gaaaaaagac aaaacttatt gaacagtgtt cctttcaaaa
     9541 aattattttg taaaaaatcg agtttttctc caaaataacg cacgcaatca aaaacaagaa
     9601 aactaacgtg ttctcctggg cgtggaactc cttatcaaga ttaccattat tgtctgtcag
     9661 aattgatatt tcaattattt catatctaga caaacccggg tgtcttgcat ttattagtac
     9721 tgtgagttta catacaaacg aggcttactc agataacata gagtcacctc taattagcaa
     9781 aaacccattt ctaattagct atttcgacca gttctaaagt caccgtaatt agaaatggtt
     9841 tttgtagctt ttctaattag aaatgacccc gagataagtg aaatatggca ttttctgggt
     9901 tttctagtaa gaaatgatcc cgcacgtagt gattccgcgt tacaagccaa attcaaatta
     9961 aaaaagtata ttacacaatt caagttacat tattttcagg aaacaatggc gaagagcaaa
    10021 gcagagattc aaaaggagga aagagacaac agatatgaac gaagacttct tgaaaagaga
    10081 aagagacatg aggtgagtaa ccatgataat aaataatcaa gtaagggcta tagatttata
    10141 aattctggag ttaacagttt cgaaaaaaag tagttttcat atgtgcgaag gaatatcaaa
    10201 aataggggaa tagcctaaac gagtgagaaa attaaatgtt tgttattttt tcaaaaatgt
    10261 ctgcagggcc gttatccttt caaatgcact ctatcaaaca tagatcatat ccatcatatc
    10321 ttatccagaa tttatatcat atccaagatt gagagcgttt caaggaaaat ctcaaacaac
    10381 catacaattt tgtagatcga caacaaggcc tccacttctt catcgatggc ctctgcagca
    10441 cctgcaccac caagtttgta tttttcatat taatataaag tgagccacgc atagtcgttt
    10501 ttaggatttt tgccagatcg atgtcgcgca aacagactgt ttttttttct ttgaaagccg
    10561 tgcgtgacat gggccacgga ttgtaacgcc gcgctggatg gataaaaatt tattcagttt
    10621 tttagccttt acaaaatatg gttcgactac tttctttatt tttacatttt tataggtgct
    10681 cattgagctg agatattttg ggtataatcg gaaaatcgct gattttggtc atttttctgc
    10741 actaaagatt acacaaaaag ttcagtattt agattcattg ttgaaaattg gggatgatct
    10801 acagatacat atcgccgtgt tcaaaaaca
//
