LOCUS       sequence-122392-        1023 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of CHD5 histone binding domain (331-672aa) with
            Y619 mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Egan CM, Nyman U, Skotte J, Streubel G, Turner S, O'Connell DJ, 
            Rraklli V, Dolan MJ, Chadderton N, Hansen K, Farrar GJ, Helin K, 
            Holmberg J, Bracken AP
  TITLE     CHD5 is required for neurogenesis and has a dual role in 
            facilitating gene expression and polycomb gene repression.
  JOURNAL   Dev Cell. 2013 Aug 12;26(3):223-36. doi: 
            10.1016/j.devcel.2013.07.008.
  PUBMED    23948251
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2013.07"; journalName: "Dev Cell"; date: 
            "2013-08-12- 12"; volume: "26"; issue: "3"; pages: "223-36"
COMMENT     Sequence Label: pCMV-FLAG-CHD5-Y619E-FRAG
FEATURES             Location/Qualifiers
     source          1..1023
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..70
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        51..69
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             111..134
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    147..171
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tagcgactct ctggctactg agaacccact gcttactggc ttatcgaaat taatacgact
       61 cactataggg agacccaagc tggctagcgt ttaaacttaa gcttaccatg gactacaagg
      121 acgacgatga caaggtacca ggatctacaa gtttgtacaa aaaagcaggc tccgaattcg
      181 cccttaggat tgatgatggt gacggctatg agacagacca ccaggattac tgtgaggtgt
      241 gccagcaggg tggggagatc atcctgtgcg acacctgccc gagggcctac catctcgtat
      301 gcctggaccc agagctggag aaggctcccg agggcaagtg gagctgcccc cactgtgaga
      361 aggaggggat ccagtgggag ccgaaggacg acgacgatga agaggaggag ggcggctgcg
      421 aggaggagga ggacgaccac atggagttct gccgcgtgtg caaggacggg ggcgagctgc
      481 tctgctgcga cgcctgcccc tcctcctacc acctgcattg cctcaacccg ccgctgcccg
      541 agatcccaaa cggtgaatgg ctctgcccgc gctgtacttg ccccccactg aagggcaaag
      601 tccagcggat tctacactgg aggtggacgg agccccctgc ccccttcatg gtggggctgc
      661 cggggcctga cgtggagccc agcctccctc cacctaagcc cctggagggc atccctgaga
      721 gagagttctt tgtcaagtgg gcagggctgt cctactggca ttgctcctgg gtgaaggagc
      781 tacagctgga gctgtaccac acggtgatgt atcgcaacta ccaaagaaag aacgacatgg
      841 atgagccgcc cccctttgac tacggctctg gggatgaaga cggcaagagc gagaagagga
      901 agaacaagga ccccctctat gccaagatgg aggagcgctt ctaccgctat ggcatcaagc
      961 cagagtggat gatgattcac cgaatcctga accatagctt tgacaagaag ggggatgtgc
     1021 act
//
