LOCUS       sequence-122397-         750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV vector containing nuclease null SaCas9 fused to VPR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Kiani S, Chavez A, Tuttle M, Hall RN, Chari R, Ter-Ovanesyan D, Qian
            J, Pruitt BW, Beal J, Vora S, Buchthal J, Kowal EJ, Ebrahimkhani MR,
            Collins JJ, Weiss R, Church G
  TITLE     Cas9 gRNA engineering for genome editing, activation and repression.
  JOURNAL   Nat Methods. 2015 Sep 7. doi: 10.1038/nmeth.3580.
  PUBMED    26344044
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 7. doi: 10.1038/nmeth.3580."
COMMENT     Sequence Label: AAV_NLS-dSaCas9-NLS-VPR
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      24..33
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             36..56
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gagctctctg gctaactacc ggtgccacca tggccccaaa gaagaagcgg aaggtcggta
       61 tccacggagt cccagcagcc aagcggaact acatcctggg cctggccatc ggcatcacca
      121 gcgtgggcta cggcatcatc gactacgaga cacgggacgt gatcgatgcc ggcgtgcggc
      181 tgttcaaaga ggccaacgtg gaaaacaacg agggcaggcg gagcaagaga ggcgccagaa
      241 ggctgaagcg gcggaggcgg catagaatcc agagagtgaa gaagctgctg ttcgactaca
      301 acctgctgac cgaccacagc gagctgagcg gcatcaaccc ctacgaggcc agagtgaagg
      361 gcctgagcca gaagctgagc gaggaagagt tctctgccgc cctgctgcac ctggccaaga
      421 gaagaggcgt gcacaacgtg aacgaggtgg aagaggacac cggcaacgag ctgtccacca
      481 aagagcagat cagccggaac agcaaggccc tggaagagaa atacgtggcc gaactgcagc
      541 tggaacggct gaagaaagac ggcgaagtgc ggggcagcat caacagattc aagaccagcg
      601 actacgtgaa agaagccaaa cagctgctga aggtgcagaa ggcctaccac cagctggacc
      661 agagcttcat cgacacctac atcgacctgc tggaaacccg gcggacctac tatgagggac
      721 ctggcgaggg cagccccttc ggctggaagg
//
