LOCUS       sequence-122398-         250 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV vector containing nuclease null SaCas9 fused to VPR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 250)
  AUTHORS   Kiani S, Chavez A, Tuttle M, Hall RN, Chari R, Ter-Ovanesyan D, Qian
            J, Pruitt BW, Beal J, Vora S, Buchthal J, Kowal EJ, Ebrahimkhani MR,
            Collins JJ, Weiss R, Church G
  TITLE     Cas9 gRNA engineering for genome editing, activation and repression.
  JOURNAL   Nat Methods. 2015 Sep 7. doi: 10.1038/nmeth.3580.
  PUBMED    26344044
REFERENCE   2  (bases 1 to 250)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 7. doi: 10.1038/nmeth.3580."
COMMENT     Sequence Label: AAV_NLS-dSaCas9-NLS-VPR
FEATURES             Location/Qualifiers
     source          1..250
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tcgttcagga aggtatccag aatctcgttc aattccgggg tcaggggtga gtccaggttc
       61 agatcctcgg tcatggactc aagtgtggtt gtcagctcat ccagatggcc ccttgggggc
      121 ggatgggaaa ggtccatttg gccacagatt gcagcctctt ccttctgggg aatcacagta
      181 tcggccatct cccgaagggc tttgacagcc tggctcgtct cttcatcggg atcctccaac
      241 aggtgactgg
//
