LOCUS       sequence-122400-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  nuclease competent SP-Cas9 fused to VPR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Kiani S, Chavez A, Tuttle M, Hall RN, Chari R, Ter-Ovanesyan D, Qian
            J, Pruitt BW, Beal J, Vora S, Buchthal J, Kowal EJ, Ebrahimkhani MR,
            Collins JJ, Weiss R, Church G
  TITLE     Cas9 gRNA engineering for genome editing, activation and repression.
  JOURNAL   Nat Methods. 2015 Sep 7. doi: 10.1038/nmeth.3580.
  PUBMED    26344044
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 7. doi: 10.1038/nmeth.3580."
COMMENT     Sequence Label: hCas9-VPR
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    113..137
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(142..711)
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
ORIGIN
        1 ctcattacta accggtaggg atcgaaccct ttgtctgctc gaagcattaa ccctcactaa
       61 agggaagcgg ccttagttat tcaggtacct gcggccgctc tagatgatat caaccacttt
      121 gtacaagaaa gctgggtttc aaaacagaga tgtgtcgaag atggacagtc ctgtgctgat
      181 atgcatggca tgcaagaggc actcgtcgtt caggaaggta tccagaatct cgttcaattc
      241 cggggtcagg ggtgagtcca ggttcagatc ctcggtcatg gactcaagtg tggttgtcag
      301 ctcatccaga tggccccttg ggggcggatg ggaaaggtcc atttggccac agattgcagc
      361 ctcttccttc tggggaatca cagtatcggc catctcccga agggctttga cagcctggct
      421 cgtctcttca tcgggatcct ccaacaggtg actggcctcg ggagtcactg cgggcgctgg
      481 atccagtggc tgagggactg gtgccggggt cagtgacccg actggctcat gtactggacc
      541 ggttggtgtt ggtgcgaggc tggcggggag tgggcggttg gcccatggac ttcctggagg
      601 atgaaatggc cggattcgtt ttggctggca cacctcgcgg ccctcaaaca cgtcactaat
      661 agcggagccg gcctcaggct tcggcaaaaa catcccttcc ctggaatccc ggctgccgct
      721 gccagagccc agcaaggctg agaaatccat atcggcgata gagctgaagt cctcgtcgcc
      781 agacagcagt ccattaggca ggcctggtgc tcccagaggg gcaggagctg gatcaggagg
      841 cctctgagcg cctgtcacga gccgggtgat ggcctcgggg tattccatca gcatgggctc
//
