LOCUS       sequence-122401-         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  nuclease competent ST1-Cas9 fused to VPR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Kiani S, Chavez A, Tuttle M, Hall RN, Chari R, Ter-Ovanesyan D, Qian
            J, Pruitt BW, Beal J, Vora S, Buchthal J, Kowal EJ, Ebrahimkhani MR,
            Collins JJ, Weiss R, Church G
  TITLE     Cas9 gRNA engineering for genome editing, activation and repression.
  JOURNAL   Nat Methods. 2015 Sep 7. doi: 10.1038/nmeth.3580.
  PUBMED    26344044
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 7. doi: 10.1038/nmeth.3580."
COMMENT     Sequence Label: M-ST1-VPR
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    98..122
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(127..696)
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
ORIGIN
        1 tagggatcga accctttgtc tgctcgaagc attaaccctc actaaaggga agcggcctta
       61 gttattcagg tacctgcggc cgctctagat gatatcaacc actttgtaca agaaagctgg
      121 gtttcaaaac agagatgtgt cgaagatgga cagtcctgtg ctgatatgca tggcatgcaa
      181 gaggcactcg tcgttcagga aggtatccag aatctcgttc aattccgggg tcaggggtga
      241 gtccaggttc agatcctcgg tcatggactc aagtgtggtt gtcagctcat ccagatggcc
      301 ccttgggggc ggatgggaaa ggtccatttg gccacagatt gcagcctctt ccttctgggg
      361 aatcacagta tcggccatct cccgaagggc tttgacagcc tggctcgtct cttcatcggg
      421 atcctccaac aggtgactgg cctcgggagt cactgcgggc gctggatcca gtggctgagg
      481 gactggtgcc ggggtcagtg acccgactgg ctcatgtact ggaccggttg gtgttggtgc
      541 gaggctggcg gggagtgggc ggttggccca tggacttcct ggaggatgaa atggccggat
      601 tcgttttggc tggcacacct cgcggccctc aaacacgtca ctaatagcgg agccggcctc
      661 aggcttcggc aaaaacatcc cttccctgga atcccggctg ccgctgccag agcccagcaa
      721 ggctgagaaa tccatatcgg cgatagagct gaagtcctcg tcgccagaca gcagtccatt
      781 aggcaggcct ggtgctccca gaggggcagg agctggatca ggaggcctct gagcgcctgt
      841 cacgagccgg gtgatggcct cggggtattc catcagcatg ggctcggtgg tgtgaggggc
      901 cacagggatg ccctggttca gcagctgctg gaactcgct
//
