LOCUS       sequence-122403-        1016 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  IPTG inducible bacterial expression of CHD5 fragment.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Egan CM, Nyman U, Skotte J, Streubel G, Turner S, O'Connell DJ, 
            Rraklli V, Dolan MJ, Chadderton N, Hansen K, Farrar GJ, Helin K, 
            Holmberg J, Bracken AP
  TITLE     CHD5 is required for neurogenesis and has a dual role in 
            facilitating gene expression and polycomb gene repression.
  JOURNAL   Dev Cell. 2013 Aug 12;26(3):223-36. doi: 
            10.1016/j.devcel.2013.07.008.
  PUBMED    23948251
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2013.07"; journalName: "Dev Cell"; date: 
            "2013-08-12- 12"; volume: "26"; issue: "3"; pages: "223-36"
COMMENT     Sequence Label: pGEX-GST-CHD5(Chromos)-FRAG
FEATURES             Location/Qualifiers
     source          1..1016
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             14..37
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    47..71
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(734..758)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(848..870)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 tctcaaatcg gatctggaag ttctgttcca ggggcccctg ggatctacaa gtttgtacaa
       61 aaaagcaggc tccgaattcg cccttcttgc cccccactga agggcaaagt ccagcggatt
      121 ctacactgga ggtggacgga gccccctgcc cccttcatgg tggggctgcc ggggcctgac
      181 gtggagccca gcctccctcc acctaagccc ctggagggca tccctgagag agagttcttt
      241 gtcaagtggg cagggctgtc ctactggcat tgctcctggg tgaaggagct acagctggag
      301 ctgtaccaca cggtgatgta tcgcaactac caaagaaaga acgacatgga tgagccgccc
      361 ccctttgact acggctctgg ggatgaagac ggcaagagcg agaagaggaa gaacaaggac
      421 cccctctatg ccaagatgga ggagcgcttc taccgctatg gcatcaagcc agagtggatg
      481 atgattcacc gaatcctgaa ccatagcttt gacaagaagg gggatgtgca ctacctgatc
      541 aagtggaaag acctgcccta cgaccagtgc acctgggaga tcgatgacat cgacatcccc
      601 tactacgaca acctcaagca ggcctactgg ggccacaggg agctgatgct gggagaagac
      661 accaggctgc ccaagaggct gctcaagaag ggcaagaagc tgagggacga caagcagtag
      721 aagggcgaat tcgacccagc tttcttgtac aaagtggtag atccccggaa ttcccgggtc
      781 gactcgagcg gccgcatcgt gactgactga cgatctgcct cgcgcgtttc ggtgatgacg
      841 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
      901 ccgggagcaa acaagcccgt cagggcgcgt caccgggtgt tggccgggtg tcggggcgca
      961 gccatgaccc aagtcacgta gcgaaacgga gtgtataatt cttgaagacg aaaggg
//
