LOCUS       sequence-122408-         768 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  storage vector for BASIC part: MB1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Storch M, Casini A, Mackrow B, Fleming T, Trewhitt H, Ellis T, 
            Baldwin GS
  TITLE     BASIC: A New Biopart Assembly Standard for Idempotent Cloning 
            Provides Accurate, Single-Tier DNA Assembly for Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2015 Apr 16.
  PUBMED    25746445
REFERENCE   2  (bases 1 to 768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 16."
COMMENT     Sequence Label: BASIC_1_MB1
FEATURES             Location/Qualifiers
     source          1..768
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      complement(80..668)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(160..179)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aatagacaga tcgctgagat aggtgcctcc ctgattaagc attggtaatc tggtgggtct
       61 ctgtcccgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
      121 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
      181 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
      241 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
      301 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
      361 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
      421 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
      481 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
      541 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
      601 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
      661 gatctcaaga agatcctttg aggctcggga gacctatcgc taaatacatt caaatatcta
      721 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaa
//
