LOCUS       sequence-12241-e        3889 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Prior JE, Lynch MD, Gill RT
  TITLE     Broad-host-range vectors for protein expression across gram negative
            hosts.
  JOURNAL   Biotechnol Bioeng. 2010 Jun 1. 106(2):326-32.
  PUBMED    20148414
REFERENCE   2  (bases 1 to 3889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2010 Jun 1. 106(2):326-32."
COMMENT     Sequence Label: pBTBXh-1
FEATURES             Location/Qualifiers
     source          1..3889
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      complement(74..110)
                     /label=soxR terminator
                     /note="bidirectional E. coli soxR transcription terminator"
     rep_origin      116..885
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             886..1548
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     CDS             join(1645..1713,1714..2505)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1863..1882)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      complement(2521..2552)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     CDS             complement(2580..3458)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        3485..3769
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     3692..3711
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 gaccatcatc atcatcatca ttagagcttg atatcattca ggacgagcct cagactccag
       61 cgtaactgga ctgaaaacaa actaaagcgc ccttgtggcg ctttagtttt gttccgcggc
      121 caccggctgg ctcgcttcgc tcggcccgtg gacaaccctg ctggacaagc tgatggacag
      181 gctgcgcctg cccacgagct tgaccacagg gattgcccac cggctaccca gccttcgacc
      241 acatacccac cggctccaac tgcgcggcct gcggccttgc cccatcaatt tttttaattt
      301 tctctgggga aaagcctccg gcctgcggcc tgcgcgcttc gcttgccggt tggacaccaa
      361 gtggaaggcg ggtcaaggct cgcgcagcga ccgcgcagcg gcttggcctt gacgcgcctg
      421 gaacgaccca agcctatgcg agtgggggca gtcgaaggcg aagcccgccc gcctgccccc
      481 cgagcctcac ggcggcgagt gcgggggttc caagggggca gcgccacctt gggcaaggcc
      541 gaaggccgcg cagtcgatca acaagccccg gaggggccac tttttgccgg agggggagcc
      601 gcgccgaagg cgtgggggaa ccccgcaggg gtgcccttct ttgggcacca aagaactaga
      661 tatagggcga aatgcgaaag acttaaaaat caacaactta aaaaaggggg gtacgcaaca
      721 gctcattgcg gcaccccccg caatagctca ttgcgtaggt taaagaaaat ctgtaattga
      781 ctgccacttt tacgcaacgc ataattgttg tcgcgctgcc gaaaagttgc agctgattgc
      841 gcatggtgcc gcaaccgtgc ggcaccctac cgcatggaga taagcatggc cacgcagtcc
      901 agagaaatcg gcattcaagc caagaacaag cccggtcact gggtgcaaac ggaacgcaaa
      961 gcgcatgagg cgtgggccgg gcttattgcg aggaaaccca cggcggcaat gctgctgcat
     1021 cacctcgtgg cgcagatggg ccaccagaac gccgtggtgg tcagccagaa gacactttcc
     1081 aagctcatcg gacgttcttt gcggacggtc caatacgcag tcaaggactt ggtggccgag
     1141 cgctggatct ccgtcgtgaa gctcaacggc cccggcaccg tgtcggccta cgtggtcaat
     1201 gaccgcgtgg cgtggggcca gccccgcgac cagttgcgcc tgtcggtgtt cagtgccgcc
     1261 gtggtggttg atcacgacga ccaggacgaa tcgctgttgg ggcatggcga cctgcgccgc
     1321 atcccgaccc tgtatccggg cgagcagcaa ctaccgaccg gccccggcga ggagccgccc
     1381 agccagcccg gcattccggg catggaacca gacctgccag ccttgaccga aacggaggaa
     1441 tgggaacggc gcgggcagca gcgcctgccg atgcccgatg agccgtgttt tctggacgat
     1501 ggcgagccgt tggagccgcc gacacgggtc acgctgccgc gccggtagta cgtaagaggt
     1561 tccaactttc accataatga aataagatca ctaccgggcg tattttttga gttatcgaga
     1621 ttttcaggag ctaaggaagc taaaatgagt attcaacatt tccgtgtcgc ccttattccc
     1681 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     1741 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     1801 aagatccttg agagtttacg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     1861 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     1921 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatctcacg
     1981 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     2041 gccaacttac ttctggcaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     2101 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     2161 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     2221 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     2281 aaagttgcag gatcacttct gcgctcggcc ctcccggctg gctggtttat tgctgataaa
     2341 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     2401 ccctcccgca tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     2461 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaatgagg atccccctca
     2521 agtcaaaagc ctccggtcgg aggcttttga ctttctgcta tggaggtcag gtatgatttt
     2581 tatgacaact tgacggctac atcattcact ttttcttcac aaccggcacg gaactcgctc
     2641 gggctggccc cggtgcattt tttaaatacc cgcgagaaat agagttgatc gtcaaaacca
     2701 acattgcgac cgacggtggc gataggcatc cgggtggtgc tcaaaagcag cttcgcctgg
     2761 ctgatacgtt ggtcctcgcg ccagcttaag acgctaatcc ctaactgctg gcggaaaaga
     2821 tgtgacagac gcgacggcga caagcaaaca tgctgtgcga cgctggcgat atcaaaattg
     2881 ctgtctgcca ggtgatcgct gatgtactga caagcctcgc gtacccgatt atccatcggt
     2941 ggatggagcg actcgttaat cgcttccatg cgccgcagta acaattgctc aagcagattt
     3001 atcgccagca gctccgaata gcgcccttcc ccttgcccgg cgttaatgat ttgcccaaac
     3061 aggtcgctga aatgcggctg gtgcgcttca tccgggcgaa agaaccccgt attggcaaat
     3121 attgacggcc agttaagcca ttcatgccag taggcgcgcg gacgaaagta aacccactgg
     3181 tgataccatt cgcgagcctc cggatgacga ccgtagtgat gaatctctcc tggcgggaac
     3241 agcaaaatat cacccggtcg gcaaacaaat tctcgtccct gatttttcac caccccctga
     3301 ccgcgaatgg tgagattgag aatataacct ttcattccca gcggtcggtc gataaaaaaa
     3361 tcgagataac cgttggcctc aatcggcgtt aaacccgcca ccagatgggc attaaacgag
     3421 tatcccggca gcaggggatc attttgcgct tcagccatac ttttcatact cccgccattc
     3481 agagaagaaa ccaattgtcc atattgcatc agacattgcc gtcactgcgt cttttactgg
     3541 ctcttctcgc taaccaaacc ggtaaccccg cttattaaaa gcattctgta acaaagcggg
     3601 accaaagcca tgacaaaaac gcgtaacaaa agtgtctata atcacggcag aaaagtccac
     3661 attgattatt tgcacggcgt cacactttgc tatgccatag catttttatc cataagatta
     3721 gcggatccta cctgacgctt tttatcgcaa ctctctactg tttctccata cccgtttttt
     3781 tgggctagca ccgcctatct cgtgtgagat aggcggagat acgaacttta agaaggagat
     3841 atacccatgg taaaatggtc agtattgagc gatatctaga gaattcgtc
//
