LOCUS       sequence-122411-         628 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  px330 with gRNA towards P53 exon 8. Cas9 is expressed from a CAG 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 628)
  TITLE     Cas9/CRISPR pig transposon 
            plasmid_LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 628)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: px330_P53 exon 8 gRNA
FEATURES             Location/Qualifiers
     source          1..628
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      9..18
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             18..83
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             90..110
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ggaccggtgc caccatggac tataaggacc acgacggaga ctacaaggat catgatattg
       61 attacaaaga cgatgacgat aagatggccc caaagaagaa gcggaaggtc ggtatccacg
      121 gagtcccagc agccgacaag aagtacagca tcggcctgga catcggcacc aactctgtgg
      181 gctgggccgt gatcaccgac gagtacaagg tgcccagcaa gaaattcaag gtgctgggca
      241 acaccgaccg gcacagcatc aagaagaacc tgatcggagc cctgctgttc gacagcggcg
      301 aaacagccga ggccacccgg ctgaagagaa ccgccagaag aagatacacc agacggaaga
      361 accggatctg ctatctgcaa gagatcttca gcaacgagat ggccaaggtg gacgacagct
      421 tcttccacag actggaagag tccttcctgg tggaagagga taagaagcac gagcggcacc
      481 ccatcttcgg caacatcgtg gacgaggtgg cctaccacga gaagtacccc accatctacc
      541 acctgagaaa gaaactggtg gacagcaccg acaaggccga cctgcggctg atctatctgg
      601 ccctggccca catgatcaag ttccgggg
//
