LOCUS       sequence-122412-         959 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transient mammalian expression of CHD5 for BirA tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Egan CM, Nyman U, Skotte J, Streubel G, Turner S, O'Connell DJ, 
            Rraklli V, Dolan MJ, Chadderton N, Hansen K, Farrar GJ, Helin K, 
            Holmberg J, Bracken AP
  TITLE     CHD5 is required for neurogenesis and has a dual role in 
            facilitating gene expression and polycomb gene repression.
  JOURNAL   Dev Cell. 2013 Aug 12;26(3):223-36. doi: 
            10.1016/j.devcel.2013.07.008.
  PUBMED    23948251
REFERENCE   2  (bases 1 to 959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2013.07"; journalName: "Dev Cell"; date: 
            "2013-08-12- 12"; volume: "26"; issue: "3"; pages: "223-36"
COMMENT     Sequence Label: pCAG-Flag-Avi-ires-CHD5
FEATURES             Location/Qualifiers
     source          1..959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             47..61
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     protein_bind    142..166
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcagatcgtt aacgctagcc tcgagccgcc accatggtac tacaaggacg acgatgacaa
       61 ggcccccttt cgttaaggca gatattggat tcacagaaaa tggaatggag gtctaatgct
      121 ggaggttcgg aattcgccct tacaagtttg tacaaaaaag caggctcttt aaaggaacca
      181 attcagtcga ctggatccgg taccgaattc atgcggggcc cagtgggcac cgaggaggag
      241 ctgccgcggc tgttcgccga ggagatggag aatgaggacg agatgtcaga agaagaagat
      301 ggtggtcttg aagccttcga tgactttttc cctgtggagc ccgtgagcct tcctaagaag
      361 aagaaaccca agaagctcaa ggaaaacaag tgtaaaggga agcggaagaa gaaagagggg
      421 agcaatgatg agctatcaga gaatgaagag gatctggaag agaagtcgga gagtgaaggc
      481 agtgactact ccccgaataa aaagaagaag aagaaactca aggacaagaa ggagaaaaaa
      541 gccaagcgaa aaaagaagga tgaggatgag gatgataatg atgatggatg cttaaaggag
      601 cccaagtcct cggggcagct catggccgag tggggcctgg acgacgtgga ctacctgttc
      661 tcggaggagg attaccacac gctgaccaac tacaaggcct tcagccagtt cctcaggcca
      721 ctcattgcca agaagaaccc gaagatcccc atgtccaaaa tgatgaccgt cctgggtgcc
      781 aagtggcggg agttcagcgc caacaacccc ttcaagggca gctccgcggc agcagcggcg
      841 gcggcggtgg ctgcggctgt agagacggtc accatctccc ctccgctagc cgtcagcccc
      901 ccgcaggtgc cccagcctgt gcctatccgc aaggccaaga ccaaggaggg caaaggcct
//
