LOCUS       sequence-12243-e        3691 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Prior JE, Lynch MD, Gill RT
  TITLE     Broad-host-range vectors for protein expression across gram negative
            hosts.
  JOURNAL   Biotechnol Bioeng. 2010 Jun 1. 106(2):326-32.
  PUBMED    20148414
REFERENCE   2  (bases 1 to 3691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2010 Jun 1. 106(2):326-32."
COMMENT     Sequence Label: pBTBXh-3
FEATURES             Location/Qualifiers
     source          1..3691
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      complement(74..110)
                     /label=soxR terminator
                     /note="bidirectional E. coli soxR transcription terminator"
     rep_origin      116..885
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             886..1548
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     CDS             1645..2304
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1711..1730)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      complement(2323..2354)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     CDS             complement(2382..3260)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        3287..3571
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     3494..3513
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 gaccatcatc atcatcatca ttagagcttg atatcattca ggacgagcct cagactccag
       61 cgtaactgga ctgaaaacaa actaaagcgc ccttgtggcg ctttagtttt gttccgcggc
      121 caccggctgg ctcgcttcgc tcggcccgtg gacaaccctg ctggacaagc tgatggacag
      181 gctgcgcctg cccacgagct tgaccacagg gattgcccac cggctaccca gccttcgacc
      241 acatacccac cggctccaac tgcgcggcct gcggccttgc cccatcaatt tttttaattt
      301 tctctgggga aaagcctccg gcctgcggcc tgcgcgcttc gcttgccggt tggacaccaa
      361 gtggaaggcg ggtcaaggct cgcgcagcga ccgcgcagcg gcttggcctt gacgcgcctg
      421 gaacgaccca agcctatgcg agtgggggca gtcgaaggcg aagcccgccc gcctgccccc
      481 cgagcctcac ggcggcgagt gcgggggttc caagggggca gcgccacctt gggcaaggcc
      541 gaaggccgcg cagtcgatca acaagccccg gaggggccac tttttgccgg agggggagcc
      601 gcgccgaagg cgtgggggaa ccccgcaggg gtgcccttct ttgggcacca aagaactaga
      661 tatagggcga aatgcgaaag acttaaaaat caacaactta aaaaaggggg gtacgcaaca
      721 gctcattgcg gcaccccccg caatagctca ttgcgtaggt taaagaaaat ctgtaattga
      781 ctgccacttt tacgcaacgc ataattgttg tcgcgctgcc gaaaagttgc agctgattgc
      841 gcatggtgcc gcaaccgtgc ggcaccctac cgcatggaga taagcatggc cacgcagtcc
      901 agagaaatcg gcattcaagc caagaacaag cccggtcact gggtgcaaac ggaacgcaaa
      961 gcgcatgagg cgtgggccgg gcttattgcg aggaaaccca cggcggcaat gctgctgcat
     1021 cacctcgtgg cgcagatggg ccaccagaac gccgtggtgg tcagccagaa gacactttcc
     1081 aagctcatcg gacgttcttt gcggacggtc caatacgcag tcaaggactt ggtggccgag
     1141 cgctggatct ccgtcgtgaa gctcaacggc cccggcaccg tgtcggccta cgtggtcaat
     1201 gaccgcgtgg cgtggggcca gccccgcgac cagttgcgcc tgtcggtgtt cagtgccgcc
     1261 gtggtggttg atcacgacga ccaggacgaa tcgctgttgg ggcatggcga cctgcgccgc
     1321 atcccgaccc tgtatccggg cgagcagcaa ctaccgaccg gccccggcga ggagccgccc
     1381 agccagcccg gcattccggg catggaacca gacctgccag ccttgaccga aacggaggaa
     1441 tgggaacggc gcgggcagca gcgcctgccg atgcccgatg agccgtgttt tctggacgat
     1501 ggcgagccgt tggagccgcc gacacgggtc acgctgccgc gccggtagta cgtaagaggt
     1561 tccaactttc accataatga aataagatca ctaccgggcg tattttttga gttatcgaga
     1621 ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat
     1681 atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc
     1741 tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag
     1801 cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa
     1861 ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac
     1921 accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat
     1981 ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc
     2041 tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt
     2101 ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc
     2161 atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat
     2221 catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc
     2281 gatgagtggc agggcggggc gtaaacgcgt ggatccccct caagtcaaaa gcctccggtc
     2341 ggaggctttt gactttctgc tatggaggtc aggtatgatt tttatgacaa cttgacggct
     2401 acatcattca ctttttcttc acaaccggca cggaactcgc tcgggctggc cccggtgcat
     2461 tttttaaata cccgcgagaa atagagttga tcgtcaaaac caacattgcg accgacggtg
     2521 gcgataggca tccgggtggt gctcaaaagc agcttcgcct ggctgatacg ttggtcctcg
     2581 cgccagctta agacgctaat ccctaactgc tggcggaaaa gatgtgacag acgcgacggc
     2641 gacaagcaaa catgctgtgc gacgctggcg atatcaaaat tgctgtctgc caggtgatcg
     2701 ctgatgtact gacaagcctc gcgtacccga ttatccatcg gtggatggag cgactcgtta
     2761 atcgcttcca tgcgccgcag taacaattgc tcaagcagat ttatcgccag cagctccgaa
     2821 tagcgccctt ccccttgccc ggcgttaatg atttgcccaa acaggtcgct gaaatgcggc
     2881 tggtgcgctt catccgggcg aaagaacccc gtattggcaa atattgacgg ccagttaagc
     2941 cattcatgcc agtaggcgcg cggacgaaag taaacccact ggtgatacca ttcgcgagcc
     3001 tccggatgac gaccgtagtg atgaatctct cctggcggga acagcaaaat atcacccggt
     3061 cggcaaacaa attctcgtcc ctgatttttc accaccccct gaccgcgaat ggtgagattg
     3121 agaatataac ctttcattcc cagcggtcgg tcgataaaaa aatcgagata accgttggcc
     3181 tcaatcggcg ttaaacccgc caccagatgg gcattaaacg agtatcccgg cagcagggga
     3241 tcattttgcg cttcagccat acttttcata ctcccgccat tcagagaaga aaccaattgt
     3301 ccatattgca tcagacattg ccgtcactgc gtcttttact ggctcttctc gctaaccaaa
     3361 ccggtaaccc cgcttattaa aagcattctg taacaaagcg ggaccaaagc catgacaaaa
     3421 acgcgtaaca aaagtgtcta taatcacggc agaaaagtcc acattgatta tttgcacggc
     3481 gtcacacttt gctatgccat agcattttta tccataagat tagcggatcc tacctgacgc
     3541 tttttatcgc aactctctac tgtttctcca tacccgtttt tttgggctag caccgcctat
     3601 ctcgtgtgag ataggcggag atacgaactt taagaaggag atatacacat gttaaaatgg
     3661 tcagtattga gcgatatcta gagaattcgt c
//
