LOCUS       sequence-12246-e       18041 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18041)
  AUTHORS   Li L, Tasic B, Micheva KD, Ivanov VM, Spletter ML, Smith SJ, Luo L
  TITLE     Visualizing the distribution of synapses from individual neurons in 
            the mouse brain.
  JOURNAL   PLoS One. 2010  . 5(7):e11503.
  PUBMED    20634890
REFERENCE   2  (bases 1 to 18041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2010  . 5(7):e11503."
COMMENT     Sequence Label: pROSA26-ZtTA
FEATURES             Location/Qualifiers
     source          1..18041
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    3..1086
                     /label=Rosa26 left arm
                     /note="left homology arm from intron 1 of the mouse Rosa26 
                     gene (Soriano, 1999)"
     enhancer        1182..1485
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1487..1764
                     /label=chicken beta-actin promoter
     intron          1766..2783
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2707..2729
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     protein_bind    complement(2975..3008)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             3087..6128
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAEFQLSAGRYHYQLVWCQ"
     CDS             6153..6956
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6216..6235)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6826..6845
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     intron          7056..7121
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    7281..7415
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    7518..7652
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    7755..7889
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(7922..7955)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(7986..8606,8607..8993)
                     /codon_start=1
                     /product="tetracycline-controlled transactivator, 
                     comprising a fusion of the tetracycline repressor TetR with
                     the C-terminal activation domain of herpes simplex virus 
                     VP16"
                     /label=tTA
                     /note="In the Tet-Off(R) system, tTA binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNYGSTI
                     EGLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAHA
                     DALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMDDFEFEQMFTDALGIDEYGG"
     primer_bind     complement(8059..8078)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     polyA_signal    9132..9253
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    9273..13545
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     promoter        13594..14087
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     14020..14039
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     intron          14762..14827
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     primer_bind     complement(14895..14912)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    14901..15125
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(15193..15213)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(15193..15211)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(15232..15248)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15232..15248)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(15245..15267)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    15256..15272
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(15280..15286,15287..15304,15305..15310))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15325..15346
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(15463..15480)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15634..16222)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15714..15733)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(16393..17184,17185..17253))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     17016..17035
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(17254..17358)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(17384..17839)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(17521..17542)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     17733..17752
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     17980..17997
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     17981..17997
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     18007..18026
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        18007..18025
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ccgcggcagg ccctccgagc gtggtggagc cgttctgtga gacagccggg tacgagtcgt
       61 gacgctggaa ggggcaagcg ggtggtgggc aggaatgcgg tccgccctgc agcaaccgga
      121 gggggaggga gaagggagcg gaaaagtctc caccggacgc ggccatggct cggggggggg
      181 ggggcagcgg aggancgctt ccggccgacg tctcgtcgct gattggcttn ttttcctccc
      241 gccgtgtgtg aaaacacaaa tggcgtgttt tggttggcgt aaggcgcctg tcagttaacg
      301 gcagccggag tgcgcagccg ccggcagcct cgctctgccc actgggtggg gcgggaggta
      361 ggtggggtga ggcgagctgn acgtgcgggc gcggtcggcc tctggcgggg cgggggaggg
      421 gagggagggt cagcgaaagt agctcgcgcg cgagcggccg cccaccctcc ccttcctctg
      481 ggggagtcgt tttacccgcc gccggccggg cctcgtcgtc tgattggctc tcggggccca
      541 gaaaactggc ccttgccatt ggctcgtgtt cgtgcaagtt gagtccatcc gccggccagc
      601 gggggcggcg aggaggcgct cccaggttcc ggccctcccc tcggccccgc gccgcagagt
      661 ctggccgcgc gcccctgcgc aacgtggcag gaagcgcgcg ctgggggcgg ggacgggcag
      721 tagggctgag cggctgcggg gcgggtgcaa gcacgtttcc gacttgagtt gcctcaagag
      781 gggcgtgctg agccagacct ccatcgcgca ctccggggag tggagggaag gagcgagggc
      841 tcagttgggc tgttttggag gcaggaagca cttgctctcc caaagtcgct ctgagttgtt
      901 atcagtaagg gagctgcagt ggagtaggcg gggagaaggc cgcacccttc tccggagggg
      961 ggaggggagt gttgcaatac ctttctggga gttctctgct gcctcctggc ttctgaggac
     1021 cgccctgggc ctgggagaat cccttgcccc ctcttccctc gtgatctgca actccagtct
     1081 ttctagcctt aattaaccgt ttaaacaatt ctgcaggaat ctagttatta atagtaatca
     1141 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     1201 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     1261 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     1321 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     1381 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     1441 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtcga ggtgagcccc
     1501 acgttctgct tcactctccc catctccccc ccctccccac ccccaatttt gtatttattt
     1561 attttttaat tattttgtgc agcgatgggg gcgggggggg ggggggggcg cgcgccaggc
     1621 ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat
     1681 cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg gcggccctat
     1741 aaaaagcgaa gcgcgcggcg ggcggggagt cgctgcgacg ctgccttcgc cccgtgcccc
     1801 gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg
     1861 tgagcgggcg ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc
     1921 ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg
     1981 gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct
     2041 ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca
     2101 gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg
     2161 ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt
     2221 cggtcgggct gcaacccccc ctgcaccccc ctccccgagt tgctgagcac ggcccggctt
     2281 cgggtgcggg gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc
     2341 ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc cggggagggc tcgggggagg
     2401 ggcgcggcgg cccccggagc gccggcggct gtcgaggcgc ggcgagccgc agccattgcc
     2461 ttttatggta atcgtgcgag agggcgcagg gacttccttt gtcccaaatc tgtgcggagc
     2521 cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg
     2581 ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc
     2641 tccctctcca gcctcggggc tgtccgcggg gggacggctg ccttcggggg ggacggggca
     2701 gggcggggtt cggcttctgg cgtgtgaccg gcggctctag agcctctgct aaccatgttc
     2761 atgccttctt ctttttccta cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc
     2821 attttggcaa agaattgatt tgataccgcg ggccccctag agcctctgct aaccatgttc
     2881 atgccttctt ctttttccta cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc
     2941 attttggcaa agaattcctc gatcgaggga cctaataact tcgtatagca tacattatac
     3001 gaagttatat taagggttcc gcaagcttcc tagactagtc gacggtatcg ataagcagct
     3061 tgatgatctg tgacatggcg gatcccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     3121 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     3181 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgct
     3241 ttgcctggtt tccggcacca gaagcggtgc cggaaagctg gctggagtgc gatcttcctg
     3301 aggccgatac tgtcgtcgtc ccctcaaact ggcagatgca cggttacgat gcgcccatct
     3361 acaccaacgt gacctatccc attacggtca atccgccgtt tgttcccacg gagaatccga
     3421 cgggttgtta ctcgctcaca tttaatgttg atgaaagctg gctacaggaa ggccagacgc
     3481 gaattatttt tgatggcgtt aactcggcgt ttcatctgtg gtgcaacggg cgctgggtcg
     3541 gttacggcca ggacagtcgt ttgccgtctg aatttgacct gagcgcattt ttacgcgccg
     3601 gagaaaaccg cctcgcggtg atggtgctgc gctggagtga cggcagttat ctggaagatc
     3661 aggatatgtg gcggatgagc ggcattttcc gtgacgtctc gttgctgcat aaaccgacta
     3721 cacaaatcag cgatttccat gttgccactc gctttaatga tgatttcagc cgcgctgtac
     3781 tggaggctga agttcagatg tgcggcgagt tgcgtgacta cctacgggta acagtttctt
     3841 tatggcaggg tgaaacgcag gtcgccagcg gcaccgcgcc tttcggcggt gaaattatcg
     3901 atgagcgtgg tggttatgcc gatcgcgtca cactacgtct gaacgtcgaa aacccgaaac
     3961 tgtggagcgc cgaaatcccg aatctctatc gtgcggtggt tgaactgcac accgccgacg
     4021 gcacgctgat tgaagcagaa gcctgcgatg tcggtttccg cgaggtgcgg attgaaaatg
     4081 gtctgctgct gctgaacggc aagccgttgc tgattcgagg cgttaaccgt cacgagcatc
     4141 atcctctgca tggtcaggtc atggatgagc agacgatggt gcaggatatc ctgctgatga
     4201 agcagaacaa ctttaacgcc gtgcgctgtt cgcattatcc gaaccatccg ctgtggtaca
     4261 cgctgtgcga ccgctacggc ctgtatgtgg tggatgaagc caatattgaa acccacggca
     4321 tggtgccaat gaatcgtctg accgatgatc cgcgctggct accggcgatg agcgaacgcg
     4381 taacgcgaat ggtgcagcgc gatcgtaatc acccgagtgt gatcatctgg tcgctgggga
     4441 atgaatcagg ccacggcgct aatcacgacg cgctgtatcg ctggatcaaa tctgtcgatc
     4501 cttcccgccc ggtgcagtat gaaggcggcg gagccgacac cacggccacc gatattattt
     4561 gcccgatgta cgcgcgcgtg gatgaagacc agcccttccc ggctgtgccg aaatggtcca
     4621 tcaaaaaatg gctttcgcta cctggagaga cgcgcccgct gatcctttgc gaatacgccc
     4681 acgcgatggg taacagtctt ggcggtttcg ctaaatactg gcaggcgttt cgtcagtatc
     4741 cccgtttaca gggcggcttc gtctgggact gggtggatca gtcgctgatt aaatatgatg
     4801 aaaacggcaa cccgtggtcg gcttacggcg gtgattttgg cgatacgccg aacgatcgcc
     4861 agttctgtat gaacggtctg gtctttgccg accgcacgcc gcatccagcg ctgacggaag
     4921 caaaacacca gcagcagttt ttccagttcc gtttatccgg gcaaaccatc gaagtgacca
     4981 gcgaatacct gttccgtcat agcgataacg agctcctgca ctggatggtg gcgctggatg
     5041 gtaagccgct ggcaagcggt gaagtgcctc tggatgtcgc tccacaaggt aaacagttga
     5101 ttgaactgcc tgaactaccg cagccggaga gcgccgggca actctggctc acagtacgcg
     5161 tagtgcaacc gaacgcgacc gcatggtcag aagccgggca catcagcgcc tggcagcagt
     5221 ggcgtctggc ggaaaacctc agtgtgacgc tccccgccgc gtcccacgcc atcccgcatc
     5281 tgaccaccag cgaaatggat ttttgcatcg agctgggtaa taagcgttgg caatttaacc
     5341 gccagtcagg ctttctttca cagatgtgga ttggcgataa aaaacaactg ctgacgccgc
     5401 tgcgcgatca gttcacccgt gcaccgctgg ataacgacat tggcgtaagt gaagcgaccc
     5461 gcattgaccc taacgcctgg gtcgaacgct ggaaggcggc gggccattac caggccgaag
     5521 cagcgttgtt gcagtgcacg gcagatacac ttgctgatgc ggtgctgatt acgaccgctc
     5581 acgcgtggca gcatcagggg aaaaccttat ttatcagccg gaaaacctac cggattgatg
     5641 gtagtggtca aatggcgatt accgttgatg ttgaagtggc gagcgataca ccgcatccgg
     5701 cgcggattgg cctgaactgc cagctggcgc aggtagcaga gcgggtaaac tggctcggat
     5761 tagggccgca agaaaactat cccgaccgcc ttactgccgc ctgttttgac cgctgggatc
     5821 tgccattgtc agacatgtat accccgtacg tcttcccgag cgaaaacggt ctgcgctgcg
     5881 ggacgcgcga attgaattat ggcccacacc agtggcgcgg cgacttccag ttcaacatca
     5941 gccgctacag tcaacagcaa ctgatggaaa ccagccatcg ccatctgctg cacgcggaag
     6001 aaggcacatg gctgaatatc gacggtttcc atatggggat tggtggcgac gactcctgga
     6061 gcccgtcagt atcggcggaa ttccagctga gcgccggtcg ctaccattac cagttggtct
     6121 ggtgtcaggg gatcccccgg gctgcagcca atatgggatc ggccattgaa caagatggat
     6181 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     6241 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     6301 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc
     6361 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     6421 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     6481 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     6541 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     6601 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     6661 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgatgat ctcgtcgtga
     6721 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     6781 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     6841 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     6901 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagggg
     6961 atcaattctc taggcttggg atctttgtga aggaacctta cttctgtggt gtgacataat
     7021 tggacaaact acctacagag atttaaagct ctaaggtaaa tataaaattt ttaagtgtat
     7081 aatgtgttaa actactgatt ctaattgttt gtgtatttta gattcacagt cccaacggct
     7141 catttcaggc ccctcagcct cacagtctgt tcatgatcat aatcagccat accacatttg
     7201 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     7261 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     7321 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     7381 ccaaactcat caatgtatct tatcatgtct ggatcataat cagccatacc acatttgtag
     7441 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     7501 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     7561 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     7621 aactcatcaa tgtatcttat catgtctgga tcataatcag ccataccaca tttgtagagg
     7681 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     7741 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     7801 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     7861 tcatcaatgt atcttatcat gtctggatcc actagttcta gctagtctag gtcgatgcag
     7921 gataacttcg tatagcatac attatacgaa gttatagatc ttgggtaccc gctcgagccg
     7981 ccaccatgtc tagattagat aaaagtaaag tgattaacag cgcattagag ctgcttaatg
     8041 aggtcggaat cgaaggttta acaacccgta aactcgccca gaagctaggt gtagagcagc
     8101 ctacattgta ttggcatgta aaaaataagc gggctttgct cgacgcctta gccattgaga
     8161 tgttagatag gcaccatact cacttttgcc ctttagaagg ggaaagctgg caagattttt
     8221 tacgtaataa cgctaaaagt tttagatgtg ctttactaag tcatcgcgat ggagcaaaag
     8281 tacatttagg tacacggcct acagaaaaac agtatgaaac tctcgaaaat caattagcct
     8341 ttttatgcca acaaggtttt tcactagaga atgcattata tgcactcagc gctgtggggc
     8401 attttacttt gggttgcgta ttggaagatc aagagcatca agtcgctaaa gaagaaaggg
     8461 aaacacctac tactgatagt atgccgccat tattacgaca agctatcgaa ttatttgatc
     8521 accaaggtgc agagccagcc ttcttattcg gccttgaatt gatcatatgc ggattagaaa
     8581 aacaacttaa atgtgaaagt gggtccgcgt acagccgcgc gcgtacgaaa aacaattacg
     8641 ggtctaccat cgagggcctg ctcgatctcc cggacgacga cgcccccgaa gaggcggggc
     8701 tggcggctcc gcgcctgtcc tttctccccg cgggacacac gcgcagactg tcgacggccc
     8761 ccccgaccga tgtcagcctg ggggacgagc tccacttaga cggcgaggac gtggcgatgg
     8821 cgcatgccga cgcgctagac gatttcgatc tggacatgtt gggggacggg gattccccgg
     8881 gtccgggatt taccccccac gactccgccc cctacggcgc tctggatatg gacgacttcg
     8941 agtttgagca gatgtttacc gatgcccttg gaattgacga gtacggtggg tagggggcgc
     9001 gaggatcccc gaattctaga taactgatca taatcagcca taccacattt gtagaggttt
     9061 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     9121 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     9181 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     9241 tcaatgtatc ttatttaaat tgggcgcgcc ggctagaaga tgggcgggag tcttctgggc
     9301 aggcttaaag gctaacctgg tgtgtgggcg ttgtcctgca ggggaattga acaggtgtaa
     9361 aattggaggg acaagacttc ccacagattt tcggttttgt cgggaagttt tttaataggg
     9421 gcaaataagg aaaatgggag gataggtagt catctggggt ttatgcagca aaactacagg
     9481 tatattgctt gtatccgcct cggagatttc catgaggaga taaagacatg tcacccgagt
     9541 ttatactctc ctgcttagat cctactacag tatgaaatac agtgtngcga ggtagactat
     9601 gtaagcagat ttaatcattt taaagagccc agtacttcat atccatttct cccgctcctt
     9661 ctgcagcctt atcaaaaggt atttagaaca ctcattttag ccccattttc atttattata
     9721 ctggcttatc caacccctag acagagcatt ggcattttcc ctttcctgat cttagaagtc
     9781 tgatgactca tgaaaccaga cagattagtt acatacacca caaatcgagg ctgtagctgg
     9841 ggcctcaaca ctgcagttct tttataactc cttagtacac tttttgttga tcctttgcct
     9901 tgatccttaa ttttcagtgt ctatcacctc tcccgtcagg tggtgttcca catttgggcc
     9961 tattctcagt ccagggagtt ttacaacaat agatgtattg agaatccaac ctaaagctta
    10021 actttccact cccatgaatg cctctctcct ttttctccat tataactgag ctatnaccat
    10081 taatggtttc aggtggatgt ctcctccccc aatatacctg atgtatctac atattgccag
    10141 gctgatattt taagacatna aaggtatatt tcattattga gccacatggt attgattact
    10201 gctactaaaa ttttgtcatt gtacacatct gtaaaaggtg gttccttttg gaatgcaaag
    10261 ttcaggtgtt tgttgtcttt cctgacctaa ggtcttgtga gcttgtattt tttctattta
    10321 agcagtgctt tctcttggac tggcttgact catggcattc tacacgttat tgctggtcta
    10381 aatgtgattt tgccaagctt cttcaggacc tataattttg cttgacttgt agccaaacac
    10441 aagtaaaatg attaagcaac aaatgtattt gtgaagcttg gtttttaggt tgttgtgttg
    10501 tgtgtgcttg tgctctataa taatactatc caggggctgg agaggtggct cggagttcaa
    10561 gagcacagac tgctcttcca gaagtcctga gttcaattcc cagcaaccac atggtggctc
    10621 acaaccatct gtaatgggat ctgatgccct cttctggtgt gtctgaagac cacaagtgta
    10681 ttcacattaa ataaataatc ctccttcttc ttcttttttt ttttttaaag agaatnctgt
    10741 ctccagtaga attactgaag taatgaaata ctttgtgttt gttccaatat ggnagccaat
    10801 aatcaaatac tcttnagcac tggaaatgta ccaaggaact attttattta agtgnactgt
    10861 ggacagagga gccataactg cagacttgtg ggatacagaa gaccaatgca gacttaatgt
    10921 cttttctctt acactaagca ataaagaaat aaaaattgaa cttctagtat cctatttgtt
    10981 aaactgctag ctttactaac ttttgtgctt catctataca aagctgaaag ctaagtctgc
    11041 agccattact aaacatgaaa gcaagtaatg ataattttgg atttcaaaaa tgtagggcca
    11101 gagtttagcc agccagtggt ggtgcttgcc tttatgcctt aatcccagca ctctggaggc
    11161 agagacaggc agatctctga gtttgagccc agcctggtct acacatcaag ttctatctag
    11221 gatagccagg aatacacaca gaaaccctgt tggggagggg ggctctgaga tttcataaaa
    11281 ttataattga agcattccct aatgagccac tatggatgtg gctaaatccg tctacctttc
    11341 tgatgagatt tgggtattat tttttctgtc tctgctgttg gttgggtctt ttgacactgt
    11401 gggctttctt aaagcctcct tccctgccat gtggtctctt gtttgctact aacttcccat
    11461 ggcttaaatg gcatggcttt ttgccttcta agggcagctg ctgagntttg cagcctgatt
    11521 tccagggtgg ggttgggaaa tctttcaaac actaaaattg tcctttaatt tttttttaaa
    11581 aaatgggtta tataataaac ctcataaaat agttatgagg agtgaggtgg actaatatta
    11641 atgagtccct cccctataaa agagctatta aggctttttg tcttatacta actttttttt
    11701 taaatgtggt atctttagaa ccaagggtct tagagtttta gtatacagaa actgttgcat
    11761 cgcttaatca gattttctag tttcaaatcc agagaatcca aattcttcac agccaaagtc
    11821 aaattaagaa tttctgactt taatgttatt tgctactgtg aatataaaat gatagctttt
    11881 cctgaggcag ggtctcacta tgtatctctg cctgatctgc aacaagatat gtagactaaa
    11941 gttctgcctg cttttgtctc ctgaatacta aggttaaaat gtagtaatac ttttggaact
    12001 tgcaggtcag attcttttat aggggacaca ctaagggagc ttgggtgata gttggtaaat
    12061 gtgtttaagt gatgaaaact tgaattatta tcaccgcaac ctacttttta aaaaaaaaag
    12121 ccaggcctgt tagagcatgc taagggatcc ctaggacttg ctgagcacac aagagtagta
    12181 cttggcaggc tcctggtgag agcatatttc aaaaaacaag gcagacaacc aagaaactac
    12241 agtaaggtta cctgtcttta accatctgca tatacacagg gatattaaaa tattccaaat
    12301 aatatttcat tcaagttttc ccccatcaaa ttgggacatg gatttctccg gtgaataggc
    12361 agagttggaa actaaacaaa tgttggtttt gtgatttgtg aaattgtttt caagtgatag
    12421 ttaaagccca tgagatacag aacaaagctg ctatttcgag gtctcttggt tatactcaga
    12481 agcacttctt tgggtttccc tgcactatcc tgatcatgtg ctaggcctnc cttaggctga
    12541 ttgttgttca aataacttaa gtttcctgtc aggtgatgtc atatgatttc atatatcaag
    12601 gcaaaacatg ttatatatgt taaacatttg nacttaatgt gaaagttagg tctttgtggg
    12661 ttttgatttt aatttcaaaa cctgagctaa ataagtcatt ttacatgtct tacatttggt
    12721 gaattgtata ttgtggtttg caggcaagac tctctgacct agtaaccctc ctatagagca
    12781 ctttgctggg tcacaagtct aggagtcaag catttcacct tgaagttgag acgttttgtt
    12841 agtgtatact agttatatgt tggaggacat gtttatccag aagatattca ggactatttt
    12901 tgactgggct aaggaattga ttctgattag cactgttagt gagcattgag tggcctttag
    12961 gcttgaattg gagtcacttg tatatctcaa ataatgctgg ccttttttna aaagcccttg
    13021 ttctttatca ccctgttttc tacataattt ttgttcaaag aaatacttgt ttggatctcc
    13081 ttttgacaac aatagcatgt tttcaagcca tatttttttt cctttttttt tttttttttg
    13141 gtttttcgag acagggtttc tctgtatagc cctggctgtc ctggaactca ctttgtagac
    13201 caggctggcc tcgaactcag aaatccgcct gcctctgcct cctgagtgcc gggattaaag
    13261 gcgtgcacca ccacgcctgg ctaagttgga tattttgtat ataactataa ccaatactaa
    13321 ctccactggg tggattttta attcagtcag tagtcttaag tggtctttat tggcccttat
    13381 taaaatctac tgttcactct aacagaggct gttggactag tggnactaag caacttccta
    13441 cggatatact agcagataag ggtcagggat agaaactagt ctagcgtttt gtatacctac
    13501 cagcttatac taccttgttc tgatagaaat atttaggaca tctagcttat cgatccgtcg
    13561 acggtatcga taagcttgat atcgaattct accgggtagg ggaggcgctt ttccaaggca
    13621 gtctgagcat gcgcttagca gccccgctgg cacttggcgc tacacaagtg gcctntggcc
    13681 tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt
    13741 cgcgccacct tctnctcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc
    13801 gtnaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtcagatg gacagcaccg
    13861 ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg
    13921 ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca
    13981 ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc
    14041 gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcaggtcct
    14101 cgccatggat cctgatgatg ttttgttatt cttctaatct tttgtatgga aaacttttct
    14161 tcgtaccacg ggactaaacc tggttatgta gattccattc aaaaaggtat acaaaagcca
    14221 aaatctggta cacaaggaaa ttatgacgat gattggaaag ggttttatag taccgacaat
    14281 aaatacgacg ctgcgggata ctctgtagat aatgaaaacc cgctctctgg aaaagctgga
    14341 ggcgtggtca aagtgacgta tccaggactg acgaaggttc tcgcactaaa agtggataat
    14401 gccgaaacta ttaagaaaga gttaggttta agtctcactg aaccgttgat ggagcaagtc
    14461 ggaacggaag agtttatcaa aaggttcggt gatggtgctt cgcgtgtagt gctcagcctt
    14521 cccttcgctg aggggagttc tagcgttgaa tatattaata actgggaaca ggcgaaagcg
    14581 ttaagcgtag aacttgagat taattttgaa acccgtggaa aacgtggcca agatgcgatg
    14641 tatgagtata tggctcaagc ctgtgcagga aatcgtgtca ggcgatctct ttgtgaagga
    14701 accttacttc tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa
    14761 ggtaaatata aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt
    14821 attttagatt ccaacctatg gaactgatga atgggagcag tggtggaatg cagatcctag
    14881 agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
    14941 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
    15001 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
    15061 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
    15121 tatggcttct gaggcggaaa gaaccagctg gggctcgacc tcgagggggg gcccggtacc
    15181 cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct
    15241 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
    15301 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
    15361 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
    15421 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
    15481 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
    15541 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
    15601 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
    15661 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
    15721 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
    15781 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
    15841 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
    15901 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
    15961 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
    16021 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
    16081 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
    16141 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
    16201 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
    16261 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
    16321 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
    16381 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
    16441 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
    16501 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
    16561 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
    16621 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
    16681 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
    16741 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
    16801 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
    16861 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
    16921 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
    16981 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
    17041 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
    17101 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
    17161 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
    17221 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
    17281 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
    17341 acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat
    17401 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
    17461 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
    17521 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
    17581 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
    17641 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
    17701 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
    17761 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
    17821 gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
    17881 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
    17941 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag
    18001 cgcgcgtaat acgactcact atagggcgaa ttggagctcc a
//
