LOCUS       sequence-122541-        9476 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  nesprin tension sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9476)
  AUTHORS   Arsenovic PT, Ramachandran I, Bathula K, Zhu R, Narang JD, Noll NA, 
            Lemmon CA, Gundersen GG, Conway DE
  TITLE     Nesprin-2G, a Component of the Nuclear LINC Complex, Is Subject to 
            Myosin-Dependent Tension.
  JOURNAL   Biophys J. 2016 Jan 5;110(1):34-43. doi: 10.1016/j.bpj.2015.11.014.
  PUBMED    26745407
REFERENCE   2  (bases 1 to 9476)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bpj.2015.11"; journalName: "Biophys J"; date: "2016-01-5-
            5"; volume: "110"; issue: "1"; pages: "34-43"
COMMENT     Sequence Label: pcdna nesprin TS
FEATURES             Location/Qualifiers
     source          1..9476
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      923..932
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2390..3097
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     CDS             join(3218..3220,3221..3223,3224..3934)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3263..3284)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3524..3543)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3871..3892
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5070..5087)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5076..5300
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5346..5774
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5433..5452)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5643..5664
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5783..5803)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5788..6117
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5968..6103
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6030..6049
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6184..6978
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6238..6257)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6848..6867
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7152..7273
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7189..7208)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7243..7262
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7322..7338)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7322..7338)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7335..7357)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7346..7362
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7370..7376,7377..7394,7395..7400))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7415..7436
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7553..7570)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7724..8309)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7804..7823)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8480..9271,9272..9340))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9103..9122
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9341..9445)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagctttcta gagccaccat ggccgctagc cctgtgctgc ccacggaaga
      961 tggagagggt ttcttgggca ttgatgatct acacttttca ctgcaagctg agcaggagga
     1021 cactcagaag aaaaccttca cctgctggat aaactcacag ctggcaaagc acacacctcc
     1081 ctcagttgta tctgacttat tcgcagacat caagaagggg catgtcctcc tggacctgtt
     1141 ggaagtcctc tcgggacagc agttgcctcg ggataaagga tctaatacat tccagtgcag
     1201 aatcaacata gaacatgccc tgacattcct gaaaaacaga tctatcaagc taataaatat
     1261 ccatgttgct gacattgtag aaggaaaccc gtccattatc cttggcctga tctggaccat
     1321 catcctgcac tttcatattg agaagctcgc tcagactctg tcttgcgatt acaatcaacc
     1381 ttcaccggag gttgtgagcg tggctgcctc atctcctacc tcaagtcctc ccactaagaa
     1441 gtgctccaag gctcaagctc aggcaagatg gcagtggtct gccaagaagg ctcttctgca
     1501 gtgggctcag gagcagtgtg ccaggtctga gtctgtcaat gtgacagatt ttaagtcaag
     1561 ctggagaaat gggatggctt ttttggctgt cattcatgcc ttgcgaccag acctgattga
     1621 catggacagc atgaggcaca gatccaacaa agacaatctg aaagaggcct tcaggatcgc
     1681 agaacatgag ctgaagatcc ccaaattgct ggaaccagaa gatgtagatg tcgtgaatcc
     1741 tgatgagaag tccatcatga cttacgtggc gcagttcctg aagtattcga aggatgcgcc
     1801 tggccccggg gattccacac aggccaaggt aagggatgct ctggtctggc taactctgca
     1861 agagaagaga ttccagaaga tgctgaagga ctcagcaagt gagacctact gtaacaagta
     1921 tcatagtctg ctgtcattta tggagtcact gaatgaagaa aaggaatcct tcattgatgt
     1981 cctgtcactg aaagggagga tgggagagct gaatgaagat gaatcacggc tgagacaagg
     2041 ctggaccagc ctcatgcacc aggtcgctgc gtggagagcc caactggatg acgccttgcc
     2101 ttcaccgctc aaagagaccg aagcgtggct gaaggacata gagggtgttg tgcaagaagg
     2161 ggtgccgacc tcccagagtt actctgaagc caggactctg atccaaggga aactatcttc
     2221 attcaagagt ctgatgggca gctttgacta tcattcggat gttctcatgg cctttcaatc
     2281 caatgccgag aagagcctgc cagctgtgcc tcctgttaaa ctggaggaga tgacaagacg
     2341 aatcaacaac gttttgggta aaaacttcat cccactcctg gagctcgaga tggtgagcaa
     2401 gggcgaggag accacaatgg gcgtaatcaa gcccgacatg aagatcaagc tgaagatgga
     2461 gggcaacgtg aatggccacg ccttcgtgat cgagggcgag ggcgagggca agccctacga
     2521 cggcaccaac accatcaacc tggaggtgaa ggagggagcc cccctgccct tctcctacga
     2581 cattctgacc accgcgttcg cctacggcaa cagggccttc accaagtacc ccgacgacat
     2641 ccccaactac ttcaagcagt ccttccccga gggctactct tgggagcgca ccatgacctt
     2701 cgaggacaag ggcatcgtga aggtgaagtc cgacatctcc atggaggagg actccttcat
     2761 ctacgagata cacctcaagg gcgagaactt cccccccaac ggccccgtga tgcagaagaa
     2821 gaccaccggc tgggacgcct ccaccgagag gatgtacgtg cgcgacggcg tgctgaaggg
     2881 cgacgtcaag cacaagctgc tgctggaggg cggcggccac caccgcgttg acttcaagac
     2941 catctacagg gccaagaagg cggtgaagct gcccgactat cactttgtgg accaccgcat
     3001 cgagatcctg aaccacgaca aggactacaa caaggtgacc gtttacgaga gcgccgtggc
     3061 ccgcaactcc accgacggca tggacgagct gtacaagggg ccaggtggtg cagggccagg
     3121 tggtgcaggg ccaggtggtg cagggccagg tggtgcaggg cccggtggtg caggtccagg
     3181 tggtgcaggt ccaggtggtg caggtccagg tggtgctatg gtgagcaagg gcgaggagct
     3241 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     3301 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagctgat
     3361 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgggctacgg
     3421 cctgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     3481 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     3541 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     3601 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     3661 ccacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat
     3721 ccgccacaac atcgaggacg gcggcgtgca gctcgccgac cactaccagc agaacacccc
     3781 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccaagct
     3841 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     3901 cgggatcact ctcggcatgg acgagctgta caaggcggcc gcgcaggagc ttcacagcaa
     3961 gctcaggctc aaacagaccg tgcagcagct caagtctgac atcggcagca tcgctgcttg
     4021 gcttgggaaa actgaggccg agctggaagc tctgaagttg gccgagcctc cctctgacat
     4081 ccaggaaata gcgctcaggg tgaagagact gcaagagata ctaaaagcct tcgataccta
     4141 caaggccttg atggtctccg tcaatgtgag ccacaaggaa tatctgccaa gccagagccc
     4201 cgaagccaca gaactccaaa atagactcca tcagctgagc ctgtcctggg actcggtcca
     4261 gggcgtattg gacagctggc gaggagactt acggcagtca ctcatgcagt gccaggactt
     4321 tcaccagctg agtcaagact tgctcctgtg gctagcgaca gcagagagcc gcaggcagaa
     4381 ggcgcatgtc accagcccag aggccgaccg ccaggtcctc ctggagtgtc agaaagacct
     4441 aatgcgccta gaaaaggagt tggtagcccg tcagcctcaa gtcagctcct tgcgggagat
     4501 ttcctccagc cttctggtga aggggcaagg agaagactac attgaggccg aagagaaggt
     4561 ccacgtgatc gagaagaaac tcaagcaact gcaagagcaa gtggctcaag atctgatgtc
     4621 cttgcagaga agcctggatc cggatgcttc tctaaccagc tttgatgagg tggactcagg
     4681 agaacagctc ccagcagcct ttgcaaagag tccaaggccc cgctggacct tcttggaaga
     4741 ggaagaggag gaggaggaga cagacagcag gatgccccac ctcgacagcc ccggcagctc
     4801 ccagccgaga cgctccttcc tctcaagggt gatcagggca gcgctaccgt tgcagctgct
     4861 tctgctgctg ctgctgctcc tggcctgcct gctacctgcc tctgaagatg actacagctg
     4921 cacccaggcc aacaactttg cccgatcctt ctaccccatg ctgcggtaca ccaacgggcc
     4981 acctcccacc tagaccggtg aattctgcag atatccagca cagtggcggc cgctcgagtc
     5041 tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     5101 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     5161 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     5221 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     5281 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
     5341 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     5401 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     5461 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     5521 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     5581 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     5641 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     5701 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     5761 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     5821 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     5881 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     5941 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     6001 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
     6061 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     6121 ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt
     6181 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
     6241 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     6301 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     6361 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     6421 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     6481 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     6541 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     6601 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     6661 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc
     6721 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
     6781 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     6841 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     6901 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     6961 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca
     7021 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     7081 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     7141 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     7201 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     7261 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
     7321 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     7381 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     7441 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     7501 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     7561 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     7621 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     7681 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     7741 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     7801 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     7861 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     7921 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     7981 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     8041 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     8101 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     8161 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     8221 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tttttttgtt tgcaagcagc
     8281 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     8341 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     8401 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     8461 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     8521 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     8581 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     8641 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     8701 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     8761 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     8821 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     8881 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     8941 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     9001 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     9061 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     9121 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     9181 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     9241 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     9301 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     9361 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     9421 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtc
//
