LOCUS       sequence-122542-         496 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  zwf targeting guide RNA E. coli , Low Phosphate Induction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 496)
  TITLE     SUPERSEDE
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 496)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCASCADE-zwf
FEATURES             Location/Qualifiers
     source          1..496
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(35..137)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 caacggtggt atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc
       61 tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac
      121 ctcttacgtg ccgatcaacg tctcattttc gccagatatc gacgtcttaa gacccacttt
      181 cacatttaag ttgtttttct aatccgcata tgatcaattc aaggccgaat aagaaggctg
      241 gctctgcacc ttggtgatca aataattcga tagcttgtcg taataatggc ggcatactat
      301 cagtagtagg tgtttccctt tcttctttag cgacttgatg ctcttgatct tccaatacgc
      361 aacctaaagt aaaatgcccc acagcgctga gtgcatataa tgcattctct agtgaaaaac
      421 cttgttggca taaaaaggct aattgatttt cgagagtttc atactgtttt tctgtaggcc
      481 gtgtacctaa atgtac
//
